FastQCFastQC Report
Fri 14 Jul 2023
SRR8545526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8545526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40832065
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length73
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA18517914.535139234324789No Hit
ACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC9153772.241809225176341No Hit
AAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA8149661.9958970970486063No Hit
AAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG5708041.3979307683801934No Hit
TCCGCCCCTTAGTGCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC5550091.3592479341909354No Hit
ATACCGAACACGGAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC5137211.2581313239974514No Hit
ATACCGAACACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCG3309280.8104610922812745No Hit
ACGGTGGATGCCTTGGCACTCTGTAGGCACCATCAATAGATCGGAAGAGC3307810.8101010810988863No Hit
TTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATC3007610.7365804301105027No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAAG2540740.6222413683951571No Hit
ATAACGGTCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2394660.5864655632772919Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGTGGCGATAGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCGG2298370.5628836063030366No Hit
ATCACCGAAGCTGCGGACTGTTCCTGTAGGCACCATCAATAGATCGGAAG2121650.5196038946352579No Hit
AGGACGCCGCCAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2116710.5183940611379807No Hit
AAATGCGATTCCCTGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC2094030.5128396028954205No Hit
ACACCGCCCGTCACACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1971160.4827480559702283No Hit
ACCGAAGCTGCGGACTGTTCCTGTAGGCACCATCAATAGATCGGAAGAGC1833950.4491445632250047No Hit
ATGAGTACGTGGTCGTAGACCCGAAACCCTGTAGGCACCATCAATAGATC1730940.42391684084554626No Hit
TGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGGA1712080.4192979218660629No Hit
ATGCAAGTCGAGCGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACA1667440.408365337388643No Hit
ACACCCGTTCCCATACCGAACCTGTAGGCACCATCAATAGATCGGAAGAG1585790.38836879790429407No Hit
AGGAGCCAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCGGA1518180.3718107325701015No Hit
ATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGAAG1487900.36439499202403797No Hit
ACAGGTCTCTGCGAAGCCGTAAGGCGAAGTCTGTAGGCACCATCAATAGA1481640.3628618831793102No Hit
TATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGAA1434070.3512117253927765No Hit
ACCCGGAGGAAGAGAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1372790.3362039122929492No Hit
TTAGGAGCCAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCG1353520.3314845820313031No Hit
TCGAACAGTGGTAGGAGAGCGTTCTCTGTAGGCACCATCAATAGATCGGA1298900.31810784000270376No Hit
ACACACCGCCCGTCACACCCTGTAGGCACCATCAATAGATCGGAAGAGCA1296660.31755925153430276No Hit
ACGTGGTCGTAGACCCGAAACCCTGTAGGCACCATCAATAGATCGGAAGA1267030.3103026996063021No Hit
ACCGATATGCTTCGGGGAGCTGTCTGTAGGCACCATCAATAGATCGGAAG1255000.3073564856443092No Hit
ATAGGAGGTAACAGCCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGC1239740.3036192267033274No Hit
ACCGAACACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCGGA1233430.30207387258028706No Hit
ACCGGGTTACCGAATTCAGTCCTGTAGGCACCATCAATAGATCGGAAGAG1232350.3018093745687366No Hit
TTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGAA1222020.2992795000693695No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1204950.2950989620534744No Hit
ACACCACGAGAGTTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1192230.2919837632507687No Hit
AGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGAA1173660.287435866885498No Hit
ATGCTTCGGGGAGCTGTAAGCCTGTAGGCACCATCAATAGATCGGAAGAG1162620.28473210943409305No Hit
ACGCGAAGAACCTTACCAGGTCCTGTAGGCACCATCAATAGATCGGAAGA1159910.2840684153495543No Hit
TAGCGAAGTTCCTGATTCCCTGTAGGCACCATCAATAGATCGGAAGAGCA1144030.28017931495749726No Hit
ATGCTTCGGGGAGCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1124560.27541100358260107No Hit
ACATGCAAGTCGAGCGGACCTGTAGGCACCATCAATAGATCGGAAGAGCA1112910.2725578537357834No Hit
ACGTGCTACAATGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1093260.2677454593589621No Hit
AGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATCAATAGATCGGAA1076080.26353798173077947No Hit
ACCCGTTCCCATACCGAACCTGTAGGCACCATCAATAGATCGGAAGAGCA1060010.2596023492811348No Hit
TCCGCCCCTTAGTGCTGCAGCTCTGTAGGCACCATCAATAGATCGGAAGA1056660.25878191563419584No Hit
ACCGGGTTACCGAATTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1026230.2513294392531948No Hit
TTAAGTTAGAAAGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1021810.2502469566503678No Hit
TCCGCCCCTTAGTGCTGCAGCTAACGCCTGTAGGCACCATCAATAGATCG1001380.24524353593187118No Hit
TCTGGTGATGATGGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATC983620.24089401307526326No Hit
TAGGAGCCAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCGG979000.23976254935918623No Hit
ACCGAACACGGAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC970420.23766125960075737No Hit
ACGTGGGTAACCTGCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA957450.23448483440648912No Hit
AAGGTTTCCTGAGGAAGGCTCGTCCGCTCAGGGTTCTGTAGGCACCATCA945210.23148719027558368No Hit
AGGCAAATCCGTTTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA922660.22596456975663612No Hit
ATAGGATATGAGAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA866110.2121151599851734No Hit
ACACGTGCTACAATGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC823660.201718918697842No Hit
AACGCGAAGAACCTTACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC820420.20092542466319058No Hit
ACGTGCTACAATGGACAGAACCTGTAGGCACCATCAATAGATCGGAAGAG809620.19828044454768576No Hit
AGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAGA799750.19586322660879388No Hit
ACCTCCTCACCATTTGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA760700.18629966424671393No Hit
ACGAAAGGCGCAACGATCTGGGCCTGTAGGCACCATCAATAGATCGGAAG752670.18433307254972287No Hit
ATCCTCTGACAATCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC745280.18252322041513208No Hit
ACGGCCCAGACTCCTACGGGAGGCAGCCTGTAGGCACCATCAATAGATCG727640.1782030862264742No Hit
ACGGAAGTTAAGCTCTTCAGCGCCGATGGTCTGTAGGCACCATCAATAGA713130.17464950646018024No Hit
ACGGAAGTTAAGCTCTTCAGCGCCCTGTAGGCACCATCAATAGATCGGAA689280.1688085087051071No Hit
TTTGGTGGCGATAGCGAAGAGGTCACACCCCTGTAGGCACCATCAATAGA678350.16613169086598975No Hit
ACGGAAGTTAAGCTCTTCAGCCTGTAGGCACCATCAATAGATCGGAAGAG676900.1657765777949266No Hit
TTAAGTTAGAAAGGGCGCACGGTGGATGCCTTGGCACTCTGTAGGCACCA666870.16332017496543463No Hit
ACGGGTTCGAATCCCGTACGGGTCACCACTGTAGGCACCATCAATAGATC662260.1621911603050201No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCCTGTAGGCACCATCAATAGATCGG662070.1621446282474325No Hit
TCCGCCCCTTAGTGCTGCAGCTAACGCATTCTGTAGGCACCATCAATAGA649940.1591739237288146No Hit
ACCGATATGCTTCGGGGAGCTGTAAGCCTGTAGGCACCATCAATAGATCG629540.1541778501773055No Hit
AAACCAAGAGGCTTGCCTCTTGGGGTTGTCTGTAGGCACCATCAATAGAT627500.1536782428221546No Hit
TCTGGTGATGATGGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCGG612180.14992628954719778No Hit
AGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC605540.1483001165872948No Hit
ATCTAAGTACCCGGAGGAAGAGAAAGCCTGTAGGCACCATCAATAGATCG604940.14815317324754454No Hit
AGACCCGGGGAACTGAAACCTGTAGGCACCATCAATAGATCGGAAGAGCA592270.1450502197231514No Hit
AATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAGAGC582580.14267708478618457No Hit
ATATCTGAATTCATAGGATATGAGAAGGCCTGTAGGCACCATCAATAGAT579690.1419693076997208No Hit
AAGCGCGGTATTGGATATCCGCGTCCAAGCCTGTAGGCACCATCAATAGA575000.1408207005940062No Hit
AAGCCGAGGCCGAAAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGC573260.14039456490873042No Hit
TAGCGAAGTTCCTGATTCCACCTGTAGGCACCATCAATAGATCGGAAGAG568490.1392263653577158No Hit
ACGTGTAGCGGTGAAATGCGTCTGTAGGCACCATCAATAGATCGGAAGAG559260.1369658869812242No Hit
AGTCGGGGGTTTCCCCCTGTGAGAGTCTGTAGGCACCATCAATAGATCGG558000.13665730596774864No Hit
TGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATAGATCGGAA539540.13213634921476541No Hit
ATCTAGTGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA530530.1299297500628489Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGGATATCCGCGTCCAAGCCTGTAGGCACCATCAATAGATCGGAAGAG526320.12889869762893452No Hit
ACTAATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAG523480.12820316582078325No Hit
ACGGAGTTACAAAGGAACGAGGTCTGTAGGCACCATCAATAGATCGGAAG515160.12616555150957953No Hit
ACACCCGTTCCCATACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA506100.12394670707935052No Hit
TAGCGAAGTTCCTGATTCCACACTGCCCTGTAGGCACCATCAATAGATCG501330.12277850752833588No Hit
AGATGAAGAGGTCTGGAAAGGCCCGCCATCTGTAGGCACCATCAATAGAT499510.12233277939776006No Hit
ATCTGAATTCATAGGATCTGTAGGCACCATCAATAGATCGGAAGAGCACA498200.12201195310597199No Hit
ACACGTGCTACAATGGACAGAACCTGTAGGCACCATCAATAGATCGGAAG496930.12170092303683393No Hit
ATAACGGTCCTAAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC488900.11973433133984283No Hit
TTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCATCTGTAGGCACCA487920.11949432388491742No Hit
AATGGAGTGGTATCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG487000.119269010763967No Hit
TTCCGCCCCTTAGTGCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCA483150.11832612433390279No Hit
ATTAAGCACTCCGCCTGGGGAGTCTGTAGGCACCATCAATAGATCGGAAG481810.11799795087512718No Hit
ACCGCCCGTCACACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG480150.11759140763515144No Hit
AAGGGCTGTGAAGCCAGACCCTGTAGGCACCATCAATAGATCGGAAGAGC457730.11210062483981646No Hit
TTTGGTGGCGATAGCGAAGAGGTCACACCCTGTAGGCACCATCAATAGAT454790.11138060247504013No Hit
ACCTCCTCACCATTTGAGCAATGGGGGGACGCCTGTAGGCACCATCAATA452050.11070956122351393No Hit
ACGGTGGATGCCTTGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA449060.10997729358042509No Hit
ACAGGTCTCTGCGAAGCCGTAAGGCGAAGTATCTGTAGGCACCATCAATA446230.10928421082793632No Hit
ACCGTGAGGGAAAGGTGAAAAGCCTGTAGGCACCATCAATAGATCGGAAG443730.10867194691231022No Hit
AAGTACCCGGAGGAAGAGAAAGCCTGTAGGCACCATCAATAGATCGGAAG443330.10857398468581005No Hit
ATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA441360.10809152072029665No Hit
AACACCCGAAGTCGGTGAGGTCTGTAGGCACCATCAATAGATCGGAAGAG433310.10612003091198056No Hit
ACCGCAAACCGACACAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCA415520.10176316088838515No Hit
ACACCCGTTCCCATACCGAACACGGAAGTTAAGCTCTTCCTGTAGGCACC410510.10053618400147041No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)