FastQCFastQC Report
Fri 14 Jul 2023
SRR8545527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8545527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24465981
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length73
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA11759644.806527071201437No Hit
AAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA8529223.486154918537703No Hit
AAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG3472601.419358577937259No Hit
TCCGCCCCTTAGTGCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC3104091.268737190632168No Hit
ACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2347110.9593361492433105No Hit
ACGGTGGATGCCTTGGCACTCTGTAGGCACCATCAATAGATCGGAAGAGC1973410.8065934490834435No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAAG1861310.7607747263434889No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGG1500850.6134436219827033No Hit
AGGACGCCGCCAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1471660.6015127699150915No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGA1431720.5851880617417302No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA1419970.580385474835446No Hit
AGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGAA1298520.5307451191104906No Hit
TTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATC1297830.5304630948581216No Hit
TTTTGGTGGCGATAGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCG1229040.5023465030893305No Hit
TTAAGTTAGAAAGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1166850.4769275346040692No Hit
ATACCGAACACGGAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1163430.4755296752662401No Hit
ATAGGATATGAGAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1069290.437051757703891No Hit
TAAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGG1068290.4366430269033561No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1037400.42401733247483514No Hit
TAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA1006580.41142024920235165No Hit
TTTGGTGGCGATAGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCGG981340.4011038837968524No Hit
TTAGGAGCCAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCG975760.3988231659298681No Hit
TTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGAA965930.3948053421606107No Hit
TTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCAT784610.32069427340763484No Hit
TTTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGA765770.31299378512555864No Hit
TGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGGA757480.30960540678912485No Hit
ATACCGAACACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCG739180.30212563313933744No Hit
ATAACGGTCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG570380.23313187400905772Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGCGATTCCCTGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC560160.2289546452275917No Hit
ACCCGGAGGAAGAGAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA558690.22835381095080554No Hit
AGGAGCCAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCGGA547750.22388229599295448No Hit
AGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAGA536730.2193780825710606No Hit
ACGTGCTACAATGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC522860.21370898636764246No Hit
ACGCGAAGAACCTTACCAGGTCCTGTAGGCACCATCAATAGATCGGAAGA515900.21086421999592006No Hit
ATGCAAGTCGAGCGGACCTGTAGGCACCATCAATAGATCGGAAGAGCACA505560.2066379435183899No Hit
ACGGTGGATGCCTTGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA478190.1954509815077515No Hit
TAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCGGAAGAGCA469920.19207077778732845No Hit
TCCGCCCCTTAGTGCTGCAGCTCTGTAGGCACCATCAATAGATCGGAAGA447930.1830827874835675No Hit
TAAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAA442830.18099826040083983No Hit
ATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGAAG441590.18049143420817665No Hit
ACCGGGTTACCGAATTCAGTCCTGTAGGCACCATCAATAGATCGGAAGAG413310.1689325271690516No Hit
ACCGAAGCTGCGGACTGTTCCTGTAGGCACCATCAATAGATCGGAAGAGC412850.16874451100080556No Hit
TAGGAGCCAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCGG411310.16811506556798192No Hit
ACCGATATGCTTCGGGGAGCTGTCTGTAGGCACCATCAATAGATCGGAAG402880.1646694649194733No Hit
ACGTGGGTAACCTGCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA402180.1643833533590989No Hit
ATCACCGAAGCTGCGGACTGTTCCTGTAGGCACCATCAATAGATCGGAAG399320.16321438326956927No Hit
TTATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGA385260.15746762821404953No Hit
TAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG384550.1571774293456698No Hit
ACGGGGAGGTTTGGCACCTCGCTGTAGGCACCATCAATAGATCGGAAGAG382830.1564744123687499No Hit
ACGTGCTACAATGGACAGAACCTGTAGGCACCATCAATAGATCGGAAGAG371570.1518721035547277No Hit
ATAGGAGGTAACAGCCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGC362000.14796054979360934No Hit
TCTGGTGATGATGGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATC358180.14639919813556626No Hit
AGGCAAATCCGTTTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA357430.14609265003516517No Hit
TCCGCCCCTTAGTGCTGCAGCTAACGCCTGTAGGCACCATCAATAGATCG354480.1448868941735874No Hit
ACACCGCCCGTCACACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA349760.142957684795063No Hit
ACCGAACACGGAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC347970.14222605666210564No Hit
ACAAAGGAACGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG345180.14108569772861346No Hit
ATATCTGAATTCATAGGATATGAGAAGGCCTGTAGGCACCATCAATAGAT336900.13770140670018505No Hit
TATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGAA334470.1367081908548854No Hit
TCGAACAGTGGTAGGAGAGCGTTCTCTGTAGGCACCATCAATAGATCGGA332870.13605422157402966No Hit
ATGCTTCGGGGAGCTGTAAGCCTGTAGGCACCATCAATAGATCGGAAGAG330890.1352449345889707No Hit
TTAAGGAGGTGTGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC318750.13028294267047785No Hit
ACACGTGCTACAATGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC318170.13004587880616764No Hit
ACCGATATGCTTCGGGGAGCTGTAAGCCTGTAGGCACCATCAATAGATCG315220.12884012294458988No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA311620.1273686920626645No Hit
TCTGGTGATGATGGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCGG311480.1273114697505896No Hit
AAGGCTGAGCTGTGATGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA311170.1271847632024238No Hit
ATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA308850.126236507745183No Hit
ATGAGTACGTGGTCGTAGACCCGAAACCCTGTAGGCACCATCAATAGATC306820.12540678422009727No Hit
AGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATCAATAGATCGGAA298810.12213285050781328No Hit
ACCGGGTTACCGAATTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA298570.12203475511568491No Hit
ACCGAACACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCGGA293360.11990526764489845No Hit
ACGGAGTTACAAAGGAACGAGGTCTGTAGGCACCATCAATAGATCGGAAG293140.11981534686878077No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATAGATCGGA290300.11865455139526185No Hit
TAATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAGAG284760.11639018276029889No Hit
ACGGAAGTTAAGCTCTTCAGCGCCGATGGTCTGTAGGCACCATCAATAGA279620.11428930644554985No Hit
ACGTGGTCGTAGACCCGAAACCCTGTAGGCACCATCAATAGATCGGAAGA279230.11412990143334126No Hit
TAGCGAAGTTCCTGATTCCCTGTAGGCACCATCAATAGATCGGAAGAGCA278280.11374160717283316No Hit
ACACCACGAGAGTTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA275180.11247454169117518No Hit
ACACCCGTTCCCATACCGAACCTGTAGGCACCATCAATAGATCGGAAGAG273940.11196771549851199No Hit
ATGCTTCGGGGAGCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA271090.11080283271698772No Hit
TACGGAGTTACAAAGGAACGAGGTCTGTAGGCACCATCAATAGATCGGAA267330.10926600490697676No Hit
TTTGGTGGCGATAGCGAAGAGGTCACACCCCTGTAGGCACCATCAATAGA266640.10898398065460771No Hit
AACACCCGAAGTCGGTGAGGTCTGTAGGCACCATCAATAGATCGGAAGAG261210.10676457240770358No Hit
TAGGCTGGGAAATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATAGA259350.10600433311870879No Hit
AAAACCAAGAGGCTTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACC259170.10593076157461251No Hit
AGTCGGGGGTTTCCCCCTGTGAGAGTCTGTAGGCACCATCAATAGATCGG249400.10193746165338721No Hit
ACAGGTCTCTGCGAAGCCGTAAGGCGAAGTCTGTAGGCACCATCAATAGA247510.10116496044037637No Hit
AAGCCGAGGCCGAAAGGCGTCTGTAGGCACCATCAATAGATCGGAAGAGC246250.10064995963170249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)