FastQCFastQC Report
Tue 11 Jul 2023
SRR8545528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8545528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9569316
Total Bases698.5 Mbp
Sequences flagged as poor quality0
Sequence length73
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA4058374.241024123354271No Hit
AAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG1050301.097570610062412No Hit
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA867680.9067314738064873No Hit
TAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA743250.7767012814709015No Hit
TAAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGG691330.7224445299956652No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGG636500.6651468088210276No Hit
TCCGCCCCTTAGTGCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC510050.5330057028109428No Hit
AGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGAA486670.5085734445387737No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC345460.36100803861007413No Hit
TTAAGTTAGAAAGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC318460.3327928558321201No Hit
AGGACGCCGCCAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG295820.3091339025694208No Hit
TTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCAT247760.25891087722466266No Hit
TTTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGA236080.24670519815627365No Hit
ACGGTGGATGCCTTGGCACTCTGTAGGCACCATCAATAGATCGGAAGAGC205230.21446673931553728No Hit
TAGGCTGGGAAATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATAGA203380.21253347679186266No Hit
TTTTGGTGGCGATAGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCG191770.2004009481973424No Hit
TAATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAGAG190860.1994499920370484No Hit
TTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATC188990.1974958293779827No Hit
TAAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAA186860.195269964958833No Hit
TGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGGA183670.1919363933639562No Hit
TAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG177590.18558275220506879No Hit
ACTCCGTCTCAAGGGTTCGAATCCCTTGCTCTCCGCCACTGTAGGCACCA162240.16954189829241711No Hit
AAGGCTGAGCTGTGATGGCGAGCGAACTGTAGGCACCATCAATAGATCGG160020.16722198326400758No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA157070.16413921329382372No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA152130.1589768798522277Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGTGGGTAACCTGCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA149030.15573735886661072No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGA147320.15395039729067364No Hit
ACTAATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAG145540.1520902852408678No Hit
AAGGCTGAGCTGTGATGGCGAGCGAAATCTGTAGGCACCATCAATAGATC135500.1415984172745471No Hit
TTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGAA134800.14086691253585942No Hit
ACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC133640.13965470468317692No Hit
ATAGGATATGAGAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA133220.13921580183996432No Hit
ATAACGGTCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG132160.13810809466423724Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGAAGTTAAGCTCTTCAGCGCCGATGGTCTGTAGGCACCATCAATAGA125470.13111699937592194No Hit
TTATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGA123300.1288493346859901No Hit
ATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA122880.12841043184277748No Hit
AGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAGA117630.12292414630261975No Hit
ATACCGAACACGGAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC107010.11182617440995783No Hit
ATAACGGTCCTAAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC104400.10909870674142227No Hit
TACGGAGTTACAAAGGAACGAGGTCTGTAGGCACCATCAATAGATCGGAA103590.10825225125808366No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAAG101790.1063712390728867No Hit
AAGCTCTTCAGCGCCGATGGTCTGTAGGCACCATCAATAGATCGGAAGAG101160.10571288480806779No Hit
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACCACTGTAGGCACCATCA96300.10063415190803605No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)