FastQCFastQC Report
Tue 11 Jul 2023
SRR8545529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8545529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16279544
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length73
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA7565844.647452041654239No Hit
AAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA4930513.0286536281360217No Hit
AAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG4847722.977798395335889No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGG1858961.1418993062704952No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAAG1178280.7237794866981532No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1115290.6850867567297954No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGA1085370.6667078635617804No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA1046150.64261627966975No Hit
TCCGCCCCTTAGTGCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC943710.5796906842108108No Hit
TGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGGA879020.5399536989488158No Hit
ACGCGAAGAACCTTACCAGGTCCTGTAGGCACCATCAATAGATCGGAAGA866410.5322077817413068No Hit
AGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAGA772730.4746631723836982No Hit
AGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGAA742060.45582357835084325No Hit
TAAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGG717570.4407801594442694No Hit
TAAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAA708800.43539303066474094No Hit
TAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA664120.4079475444766757No Hit
TAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG647060.39746813547111637No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA450440.2766907967446754No Hit
ACGGGGAGGTTTGGCACCTCGCTGTAGGCACCATCAATAGATCGGAAGAG390430.23982858487928163No Hit
AGGACGCCGCCAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG388970.2389317538623932No Hit
ATAACGGTCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG347030.21316936150054325Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATAGATCGGA332620.20431776221741837No Hit
TTAAGTTAGAAAGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC321120.19725368228987247No Hit
TTAAGGAGGTGTGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC278770.17123944012191006No Hit
ACGGTGGATGCCTTGGCACTCTGTAGGCACCATCAATAGATCGGAAGAGC273960.1682848119087365No Hit
TTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGAA269710.16567417367464346No Hit
TTTGGTGGCGATAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG252250.15494905754116944No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA212880.1307653334761711Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC209630.12876896306186464No Hit
TACAGGAGGTGTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC199630.12262628486399865No Hit
ACTAATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAG197580.12136703583343611No Hit
TTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATC195910.12034120857439251No Hit
ACGTGGGTAACCTGCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA189930.11666788701206865No Hit
ATAGGATATGAGAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA188400.11572805724779514No Hit
TTTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGA188070.11552534886726556No Hit
ACGCGAAGAACCTTACCAGGTCTCTGTAGGCACCATCAATAGATCGGAAG187950.11545163672889117No Hit
ACAGGAGGTGTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG184240.11317270311748288No Hit
TCCGCCCCTTAGTGCTGCAGCTCTGTAGGCACCATCAATAGATCGGAAGA178930.10991094099441606No Hit
TAGGCTGGGAAATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATAGA177920.10929053049643159No Hit
TAATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAGAG177230.10886668570077884No Hit
ATCTAGTGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA174280.10705459563240838Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGGAAGAG164600.1011084831368741No Hit
ATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA163730.10057407013365977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)