FastQCFastQC Report
Fri 14 Jul 2023
SRR8545530_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8545530_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13356356
Total Bases975 Mbp
Sequences flagged as poor quality0
Sequence length73
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA6663574.98906288511627No Hit
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA2269681.6993257741857135No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1311230.9817273513823681No Hit
AGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGAA1075820.8054741877200637No Hit
AAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG996810.7463188312740391No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA981410.7347887402821548Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGG665760.4984593103088897No Hit
TAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA591930.4431822571964988No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGG450420.3372327002963982No Hit
ACGGGGAGGTTTGGCACCTCGCTGTAGGCACCATCAATAGATCGGAAGAG435260.3258822990342575No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA381140.28536226497706413No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAAG349160.26141860848872256No Hit
TGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGGA324930.24327743285668638No Hit
TCCGCCCCTTAGTGCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC265030.19842987114149996No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGA231770.17352786942785892No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA204070.15278867978661245No Hit
ACGGTCCTAAGGTAGCGAACTGTAGGCACCATCAATAGATCGGAAGAGCA200900.15041527793958173No Hit
AAGGCTGAGCTGTGATGGCGAGCGAACTGTAGGCACCATCAATAGATCGG200870.15039281672336377No Hit
ACGTGGGTAACCTGCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA200050.1497788768134063No Hit
TTAAGGAGGTGTGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC197060.14754024226368329No Hit
ACGCGAAGAACCTTACCAGGTCCTGTAGGCACCATCAATAGATCGGAAGA191580.1434373267678699No Hit
ACGCGAAGAACCTTACCAGGTCTCTGTAGGCACCATCAATAGATCGGAAG175190.13116601564079305No Hit
ACAAACCGGAGGAAGGTGGGGCTGTAGGCACCATCAATAGATCGGAAGAG158480.11865511820739129No Hit
AGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAGA156540.11720262622529679No Hit
TTAAGTTAGAAAGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC155800.11664858289192052No Hit
ATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA149730.11210393014382067No Hit
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACCACTGTAGGCACCATCA137300.10279749955751404No Hit
ATATAGGAGGTGAGCTACCTGTAGGCACCATCAATAGATCGGAAGAGCAC135870.10172684825112477No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)