FastQCFastQC Report
Tue 11 Jul 2023
SRR8545531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8545531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13029497
Total Bases951.1 Mbp
Sequences flagged as poor quality0
Sequence length73
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA4940663.7919038624438075No Hit
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA1944761.4925825609384613No Hit
AAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG1758131.3493460261743029No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGG1350441.0364482988100001No Hit
AGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGAA674390.5175871332561802No Hit
TAAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGG662410.5083926110117681No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC655600.5031660086341015No Hit
TAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA629020.4827661420851473No Hit
TGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGGA605610.4647992167310834No Hit
TCCGCCCCTTAGTGCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC486820.3736291585162497No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA456100.3500518861165554Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGA331220.25420781784592295No Hit
TTAAGTTAGAAAGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC314910.24169006677694466No Hit
TTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGAA309540.23756864904301372No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGA263900.20254043575128033No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAAG262270.20128942813371845No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA250710.19241725141039598No Hit
AGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAGA245470.1883956072901356No Hit
TAAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAA243840.18714459967257369No Hit
TAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG214660.1647492608502078No Hit
ATAGGCAAATCCGTTTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA208730.16019804908815743No Hit
ACGGGGAGGTTTGGCACCTCGCTGTAGGCACCATCAATAGATCGGAAGAG199900.15342111825191715No Hit
TTAAGGAGGTGTGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC194420.14921527669103418No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA185950.14271464201572787No Hit
ACGGTGGATGCCTTGGCACTCTGTAGGCACCATCAATAGATCGGAAGAGC177680.13636750520760701No Hit
AGGACGCCGCCAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG173750.13335127211741174No Hit
TTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATC171800.13185466791235304No Hit
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACCACTGTAGGCACCATCA153270.11763309051761553No Hit
TTTTGGTGGCGATAGCGAAGAGGTCACCTGTAGGCACCATCAATAGATCG150670.1156376182442039No Hit
TCTACAGGAGGTGTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC148710.1141333391457859No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATAGATCGGA139610.1071491861888452No Hit
ACGGTCCTAAGGTAGCGAACTGTAGGCACCATCAATAGATCGGAAGAGCA138190.10605935133182808No Hit
ACTAATCGATCGAGGACTTAACCCTGTAGGCACCATCAATAGATCGGAAG136810.10500021604824805No Hit
ACGTGGGTAACCTGCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA133840.10272077272054324No Hit
TTTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCAT132670.101822810197508No Hit
TTAGGAGCCAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCG131490.1009171727811135No Hit
ATAACGGTCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG130620.10024945705885653Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)