FastQCFastQC Report
Mon 17 Jul 2023
SRR8545532_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8545532_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23216956
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length73
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA8684283.740490355410933No Hit
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA8424783.6287185968737674No Hit
AAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG4558831.9635778264816457No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1579810.6804552672624267No Hit
TAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA1499060.6456746526116516No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA1491870.6425777780687528No Hit
TAAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGG1408300.6065825339032387No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGA1379730.5942768724719985No Hit
ACGCGAAGAACCTTACCAGGTCCTGTAGGCACCATCAATAGATCGGAAGA1223740.5270889086407365No Hit
AGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGAA1199210.5165233547412503No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAAG1178950.507796973901316No Hit
ACGGGGAGGTTTGGCACCTCGCTGTAGGCACCATCAATAGATCGGAAGAG893890.38501602018800396No Hit
TCCGCCCCTTAGTGCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC828090.35667466484409066No Hit
ACGGTGGATGCCTTGGCACTCTGTAGGCACCATCAATAGATCGGAAGAGC787320.33911422324270246No Hit
TAAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAA773070.33297646771609507No Hit
TAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG763260.3287511075956727No Hit
ACGCGAAGAACCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT681970.2937379043144157No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCCTGTAGGCACCATCAATAGATCGG678490.2922389998068653No Hit
AGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAGA652600.2810876671343134No Hit
ACGGTGGATGCCTTGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA649470.27973951451689016No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGG629720.2712328007168554No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA534580.23025412978342208No Hit
AGGACGCCGCCAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG523900.22565404353611213No Hit
TAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC514150.22145452659685447TruSeq Adapter, Index 5 (100% over 35bp)
ACGGTCCTAAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC499540.21516171198325915No Hit
ACGTGGGTAACCTGCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA453810.19546490073892547No Hit
ACGCGAAGAACCTTACCAGGTCTCTGTAGGCACCATCAATAGATCGGAAG430630.18548081841564415No Hit
ACGGGGAGGTTTGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG385300.16595629504574158No Hit
TGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGAAGAGC379270.163359055338693No Hit
AAGGCTGAGCTGTGATGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA376680.1622434913517517No Hit
ATAACGGTCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG376600.1622090337768655Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGCTGAGCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC374870.16146388871995104No Hit
TGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC340310.1465782163691054No Hit
ACAGGAGGTGTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG337280.14527313572028994No Hit
ACAAAGGAACGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG334420.14404127741810768No Hit
ACGCGAGCTGGGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG324510.1397728453290776No Hit
TGCCTCTTGGGGTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC317760.13686548744805305No Hit
AATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTC294020.12664020210056823TruSeq Adapter, Index 25 (97% over 44bp)
ACGCTGGCGGCGTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC292690.12606734491808486No Hit
TTAAGGAGGTGTGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC283270.12200996547523284No Hit
AACGCGAAGAACCTTACCAGGTCCTGTAGGCACCATCAATAGATCGGAAG271440.11691455158893353No Hit
TAACCGGAGGAAGGTGGGGACTGTAGGCACCATCAATAGATCGGAAGAGC260430.11217232784521795No Hit
ACGGCCCAGACTCCTACGGGAGGCCTGTAGGCACCATCAATAGATCGGAA259420.11173730096227946No Hit
ACTGGGACTGAGACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT252070.1085715112696083No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA249790.10758947038535113Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAACGATGAGTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT249390.10741718251092004No Hit
ACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC249000.10724920183334972No Hit
ACGGGGAGGTTTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT247130.10644375602038442No Hit
TGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGGA242480.10444090948012308No Hit
ATCGGAGAGTTTGATCCTGGCTCCTGTAGGCACCATCAATAGATCGGAAG240730.10368715002948706No Hit
AAGGCTGAGCTGTGATGGCGAGCTGTAGGCACCATCAATAGATCGGAAGA233070.1003878372341318No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)