FastQCFastQC Report
Fri 14 Jul 2023
SRR8545533_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8545533_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13009145
Total Bases949.6 Mbp
Sequences flagged as poor quality0
Sequence length73
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA6835655.254495971872094No Hit
AAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA2348521.805283898365342No Hit
ACGGTCCTAAGGTAGCGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1739481.337120925318305No Hit
ACGCGAAGAACCTTACCAGGTCCTGTAGGCACCATCAATAGATCGGAAGA1574731.2104792436397627No Hit
AAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG1507131.1585157979252287No Hit
TTGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGG1437531.105014972159969No Hit
AGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAAG1172720.9014581665436122No Hit
TGCCTCTTGGGGTTGTAGGACACTCCTGTAGGCACCATCAATAGATCGGA977750.7515866722986022No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA854020.6564766554604472No Hit
TAAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGA754270.5797998254304952No Hit
TCCGCCCCTTAGTGCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC724560.5569620447769627No Hit
TAGGCTGAGCTGTGATGGCGAGCGCTGTAGGCACCATCAATAGATCGGAA692290.532156417658501No Hit
TTAAGTTAGAAAGGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC449510.3455338533008895No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCATGTGGCTGTAGGCACCATCAATA446700.34337383432961965No Hit
AGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGAA413040.3174997280759035No Hit
AGGACGCCGCCAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG344380.26472147093448495No Hit
ACGGGGAGGTTTGGCACCTCGCTGTAGGCACCATCAATAGATCGGAAGAG332850.2558584749420504No Hit
ACGGTGGATGCCTTGGCACTCTGTAGGCACCATCAATAGATCGGAAGAGC306000.2352191477610558No Hit
TTGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATAGATCGGA282010.21677827405259917No Hit
AGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAGA278580.2141416672656043No Hit
TTTGGTGGCGATAGCGAAGAGGTCACACCCGTTCCCCTGTAGGCACCATC278340.2139571816595172No Hit
TTAAGGAGGTGTGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC270100.2076231758505267No Hit
TAAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGG260360.20013613500349178No Hit
AACGCGAAGAACCTTACCAGGTCCTGTAGGCACCATCAATAGATCGGAAG255660.19652329188428602No Hit
ACAGGAGGTGTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG254950.19597752196627832No Hit
ACGCGAAGAACCTTACCAGGTCTGTAGGCACCATCAATAGATCGGAAGAG253660.19498591183356018No Hit
TAGGCTGAGCTGTGATGGCGAGCGACTGTAGGCACCATCAATAGATCGGA243190.1869377272680103No Hit
TTTGGTGGCGATAGCGAAGAGGTCCTGTAGGCACCATCAATAGATCGGAA224650.17268621419778166No Hit
TACAGGAGGTGTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC224110.17227112158408564No Hit
ACGGGGGCCCGCACAAGCGGTGGAGCCTGTAGGCACCATCAATAGATCGG220890.16979593970241702No Hit
ATGCCTAAGGTTTCCTGAGGCTGTAGGCACCATCAATAGATCGGAAGAGC216340.1662984000870157No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA184240.14162345027286574Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTAAGGAGGTGTGTCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA182290.14012450472340804No Hit
TGCCTCTTGGGGTTGTAGGACACTCTGTAGGCACCATCAATAGATCGGAA177490.13643479260166597No Hit
ACCGGGGCTAAACGTATCACCGAAGCTGCGGACTGTTCCTGTAGGCACCA176650.13578909298036113No Hit
TCAAGGAGGTGCGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC173300.13321398139539534No Hit
ACGGTCCTAAGGTAGCGAACTGTAGGCACCATCAATAGATCGGAAGAGCA173250.13317554689412717No Hit
TAAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAA173080.13304486958981548No Hit
ACGGTGGATGCCTTGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA171720.13199945115532188No Hit
ACCGAAGCTGCGGACTGTTCCTGTAGGCACCATCAATAGATCGGAAGAGC168160.12926291466502987No Hit
ACGACGTGGGGGTTCGAGTCCCTTCACCCGCACCACTGTAGGCACCATCA161950.12448934960752608No Hit
ACGCTGGCGGCGTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC152780.11744046207494804No Hit
ATCACCGAAGCTGCGGACTGTTCCTGTAGGCACCATCAATAGATCGGAAG152740.11740971447393353No Hit
TAGGCTGAGCTGTGATGGCGAGCCTGTAGGCACCATCAATAGATCGGAAG152300.11707149086277384No Hit
ACGTGGGTAACCTGCCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA149140.114642430382627No Hit
ACGGAAGTTAAGCTCTTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC143940.11064524225073977No Hit
ATGTGGTTTAATTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG142460.10950758101320264No Hit
AACCGGAGGAAGGTGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC140910.10831611147389009No Hit
ACGGAAGTTAAGCTCTTCAGCGCCGATGGTCTGTAGGCACCATCAATAGA138690.10660961961758439No Hit
TTAGGAGCCAGCCGCCGAAGGTGGGACCTGTAGGCACCATCAATAGATCG135230.10394995212982869No Hit
ACGCGAAGAACCTTACCAGGTCTCTGTAGGCACCATCAATAGATCGGAAG134510.10339649531156737No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)