FastQCFastQC Report
Fri 22 Sept 2023
SRR8558080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8558080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496037564
Total Bases37.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG197484893.9812486862386094Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA65911851.3287673108563205RNA PCR Primer, Index 1 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG58536811.1800882483166135No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA54499771.0987024764922844No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA54118181.0910097123209RNA PCR Primer, Index 1 (100% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG53218211.0728665299227218No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG50857331.0252717473630686No Hit
CCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG49708161.0021047518893145RNA PCR Primer, Index 1 (100% over 29bp)
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGTGGAATTCTCGGGTGC47082950.94918113903164No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAA43144560.8697841278810893No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG32359830.6523665211774163No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA31643030.6379160026678947RNA PCR Primer, Index 1 (100% over 23bp)
CGGCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA30698110.6188666388983396RNA PCR Primer, Index 1 (100% over 37bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC30451080.6138865725096577RNA PCR Primer, Index 1 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA30346960.6117875379292848RNA PCR Primer, Index 1 (100% over 22bp)
CGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAAC29631730.5973686702485298RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG26496230.5341577316511457Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA23669090.477163257740698RNA PCR Primer, Index 1 (100% over 32bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA23036530.4644109977122619No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA22727580.4581826387648335RNA PCR Primer, Index 1 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA21667250.43680663668447495RNA PCR Primer, Index 1 (100% over 23bp)
CGGTCCCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAA20921870.42177995213281866RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA20161040.4064417992343822RNA PCR Primer, Index 1 (100% over 22bp)
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGGAATTCTCGGGTG18587360.3747167825378644No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA17414220.3510665575319211No Hit
CGCGGGCCGGTCCCCCCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAAC17136710.34547202155036794RNA PCR Primer, Index 1 (100% over 24bp)
AGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC16825010.3391882232531889RNA PCR Primer, Index 1 (100% over 33bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG16711720.3369043236411023Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA16118690.3249489790656258RNA PCR Primer, Index 1 (100% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG16094450.32446030639727924Illumina Small RNA Adapter 2 (100% over 21bp)
AACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTGCCAAGG15663170.3157658035753115Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG15552470.3135341177508081Illumina Small RNA Adapter 2 (100% over 21bp)
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT15334760.3091451356292847RNA PCR Primer, Index 1 (100% over 30bp)
AGGGGCCCGGGGCGGGGTCCGCCGGCTGGAATTCTCGGGTGCCAAGGAAC13364460.2694243535152914RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG13299880.2681224359855134No Hit
CGCGGGCCGGTCCCCCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACT13070000.2634881095416395RNA PCR Primer, Index 1 (100% over 25bp)
CGGGCCGGTCCCCCCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAACTC12384030.24965911654223025RNA PCR Primer, Index 1 (100% over 26bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA12144170.24482359565817075RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA11668320.2352305721749734RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA11446020.23074905673877555RNA PCR Primer, Index 1 (100% over 23bp)
ACGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAA11395620.2297330046560748RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGG11222660.22624617195321925Illumina Small RNA Adapter 2 (100% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGA9794850.19746185996510537RNA PCR Primer, Index 1 (100% over 22bp)
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC9715190.19585593320105896RNA PCR Primer, Index 1 (100% over 36bp)
GAGACGCGACCTCAGATCAGACGTGGCGTGGAATTCTCGGGTGCCAAGGA9260270.18668485356887204RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA9154240.18454731384012685No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA9115440.18376511501455564No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG8969470.18082239432979716No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCATGGAATTCTCGGGTGCCAAG8599890.173371748918596No Hit
CCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA8125920.1638166257908645RNA PCR Primer, Index 1 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTGGAATTCTCGGGTGCCAAGGAAC7898650.15923491632984474RNA PCR Primer, Index 1 (100% over 24bp)
CGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGTGGAATTCTCGGGTGCCA7709370.1554190762859242No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG7606980.15335491809648513No Hit
GTCAGGATGGCCGAGTGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC7382750.14883449431664414RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGAA7314250.14745355051376713RNA PCR Primer, Index 1 (100% over 23bp)
AGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT7301750.14720155346944652RNA PCR Primer, Index 1 (100% over 35bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGTGGAATTCTCGGGTGCCAAG6929970.13970655657844494No Hit
CCCCGCGAGGGGGGCCCGGGCACTGGAATTCTCGGGTGCCAAGGAACTCC6739140.13585946890102865RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG6453760.13010627558037116Illumina Small RNA Adapter 2 (100% over 21bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG6446300.12995588374432063No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA6335180.12771573081912804RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA6198380.1249578751660832RNA PCR Primer, Index 1 (100% over 37bp)
AGCGGCCCCCGGCGCGCCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC6163810.12426095214031008RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG6052970.12202644394891028Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGAGGACAGAAACCTCCCGTGGAGCTGGAATTCTCGGGTGCCAAGGAA6036870.12170187175582534RNA PCR Primer, Index 1 (100% over 23bp)
ATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA5825780.11744634726897417RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCA5511080.11110206968115825No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA5330150.1074545636628439No Hit
ATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG5178890.10440519782892894Illumina Small RNA Adapter 2 (100% over 21bp)
GCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGTGGAATTCTCGGGTGCC5169980.10422557433573719No Hit
CCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAACTCCA5077230.1023557562668782RNA PCR Primer, Index 1 (100% over 28bp)
GTCAGGATGGCCGAGCGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC5019240.10118669157886599RNA PCR Primer, Index 1 (100% over 24bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG4961810.10002891635843933No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)