FastQCFastQC Report
Sat 23 Sept 2023
SRR8558081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8558081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411820853
Total Bases30.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG102687492.4934990360966496Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCCATGTGGAATTCTCGGGTGC54433851.3217846935983109No Hit
CACGCGGGAGGCCCGGGTTCGTTTCCCGGCCCATGTGGAATTCTCGGGTG39767370.9656473126677731No Hit
CCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG32927020.7995471759172914RNA PCR Primer, Index 1 (100% over 29bp)
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA30213770.7336629454264183RNA PCR Primer, Index 1 (100% over 22bp)
CGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAAC29271980.7107940209137491RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC28250860.6859987733549763RNA PCR Primer, Index 1 (100% over 27bp)
CGGCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT26229230.636908738567447RNA PCR Primer, Index 2 (100% over 37bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA25919070.6293773083899663RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCCATGTGGAATTCTCGGGTGCC25256080.6132783178903279No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA24955600.6059819413758536RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCA24370230.5917677510128415No Hit
AGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC24166990.5868325953858388RNA PCR Primer, Index 1 (100% over 33bp)
CGCGGGCCGGTCCCCCCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAAC19906660.4833815445474783RNA PCR Primer, Index 1 (100% over 24bp)
AGGCGGCGGAGGGGCCGCGGGCCGGTCCCCCCCGCCGGGTCTGGAATTCT17744670.43088323164635867No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA17068290.41445909976783035No Hit
AGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA16313350.3961273422936648RNA PCR Primer, Index 1 (100% over 32bp)
ACGCGGGAGGCCCGGGTTCGATTCCCGGCCCATGTGGAATTCTCGGGTGC16092600.3907670017865754No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA15494420.37624175383853137RNA PCR Primer, Index 1 (100% over 23bp)
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGTGGAATTCTCGGGTGC15074820.366052857454501No Hit
GAGACGCGACCTCAGATCAGACGTGGCGTGGAATTCTCGGGTGCCAAGGA14775950.3587955756091836RNA PCR Primer, Index 1 (100% over 22bp)
CACGCGGGAGGCCCGGGTTCGATTCCCGGCCCATGTGGAATTCTCGGGTG14493100.35192729786318033No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA13942140.33854866499438774RNA PCR Primer, Index 1 (100% over 23bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG13035200.3165259822333475Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG13027040.31632783782321No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG12764400.30995030744594176No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA12316030.2990628063217576RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGCCGGTCCCCCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACT11754460.28542653715497984RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA10776930.2616897595518311RNA PCR Primer, Index 1 (100% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG10763640.26136704641326164No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC10474860.2543547740162638No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG9877560.23985089458303852Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGAGACCCGGGTTCATTTCCCGGCCTGGAATTCTCGGGTGCCAAGG9822960.238525075368148Illumina Small RNA Adapter 2 (100% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGA9395710.22815041859961374RNA PCR Primer, Index 1 (100% over 22bp)
ATGGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGAAC9210320.2236487038697868RNA PCR Primer, Index 1 (100% over 24bp)
AGGGGCCCGGGGCGGGGTCCGCCGGCTGGAATTCTCGGGTGCCAAGGAAC9173200.22274734106288688RNA PCR Primer, Index 1 (100% over 24bp)
ACGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAA8671600.21056728761620044RNA PCR Primer, Index 1 (100% over 23bp)
AGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG8593190.20866330438104358RNA PCR Primer, Index 2 (100% over 35bp)
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGTGGAATTCTCGGGTGCC8300840.2015643438046106No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA8277160.20098933649676065RNA PCR Primer, Index 1 (100% over 23bp)
ACGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGA8245910.20023051139666306RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCC8209580.1993483316882936No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA7843950.19046995660513577RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC7760170.18843557686477813RNA PCR Primer, Index 2 (100% over 42bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA7593410.18438624330662537RNA PCR Primer, Index 1 (100% over 22bp)
ATGGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAACT7536060.1829936474829263RNA PCR Primer, Index 1 (100% over 25bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG7530370.18285548060870052Illumina Small RNA Adapter 2 (100% over 21bp)
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT7249570.17603698178926358RNA PCR Primer, Index 1 (100% over 30bp)
CGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGGGTGGAATTCT7191360.17462350309880983No Hit
CTCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGGAATTCTCG7108360.17260806363295061No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCTGGAATTCTCGGGTGCCAAGG7108310.17260684951279046Illumina Small RNA Adapter 2 (100% over 21bp)
CGGTCCCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAA6787630.16481996845361302RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA6414820.15576724571545678No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA6281790.15253695761734534No Hit
GCCCGGCTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT6253710.15185510773540165RNA PCR Primer, Index 2 (100% over 37bp)
ACGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGTGGAATTCTCGGGTGC5929160.1439742537758281No Hit
CGGGCCGGTCCCCCCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAACTC5748440.13958593786895973RNA PCR Primer, Index 1 (100% over 26bp)
AGACGCGACCTCAGATCAGACGTGGCGTGGAATTCTCGGGTGCCAAGGAA5482750.13313434616192202RNA PCR Primer, Index 1 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTGGAATTCTCGGGTGCCAAGGAAC5405380.1312556166260964RNA PCR Primer, Index 1 (100% over 24bp)
ATCCCGGACGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT5388140.13083698799487456RNA PCR Primer, Index 2 (100% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA5134930.12468844067981182RNA PCR Primer, Index 1 (100% over 22bp)
GGGGATGTAGCTCAGTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5093310.12367780705849785RNA PCR Primer, Index 1 (100% over 31bp)
ATCCCGGACGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT5068240.12306904721019554RNA PCR Primer, Index 2 (100% over 39bp)
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAA4921490.11950560454013727No Hit
GTCAGGATGGCCGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC4920740.11948739273773491RNA PCR Primer, Index 2 (100% over 34bp)
ACGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGG4889620.11873172435005375Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGG4838510.11749065072234213Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG4825570.1171764364248937No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA4812130.11685008092584374RNA PCR Primer, Index 1 (100% over 23bp)
GTCAGGATGGCCGAGTGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC4803460.11663955249007266RNA PCR Primer, Index 1 (100% over 24bp)
ATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA4366270.10602352863369938RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGAGGCCCGGGTTCGATTCCCGGCCCATGTGGAATTCTCGGGTGCC4353690.10571805600140408No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGGAATTCTCGG4353110.10570397220754627No Hit
CTGTCCCCGCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4313220.1047353471437737RNA PCR Primer, Index 1 (100% over 32bp)
CGCGGGAGACCCGGGTTCATTTCCCGGCCAATGTGGAATTCTCGGGTGCC4260440.10345372190271288No Hit
CCCCGCGAGGGGGGCCCGGGCACTGGAATTCTCGGGTGCCAAGGAACTCC4236840.10288065718711918RNA PCR Primer, Index 1 (100% over 27bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCTGGAATTCTCGGGTGCCAAGGA4184170.10160170301041069RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)