FastQCFastQC Report
Fri 8 Sept 2023
SRR8558082_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8558082_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406326972
Total Bases30.4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG137216493.3769968388906264Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA52962641.3034487900055034No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAA37590850.9251379453097197No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA33953410.8356179220118325RNA PCR Primer, Index 1 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA32120250.7905025315425036RNA PCR Primer, Index 1 (100% over 22bp)
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGTGGAATTCTCGGGTGC28369020.6981820542299614No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG28285670.6961307505818246No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG26984590.6641102328791503No Hit
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC24181150.5951155514234482RNA PCR Primer, Index 1 (100% over 27bp)
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA23877960.5876538267314433RNA PCR Primer, Index 1 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG22864010.5626997855313429No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG22338120.5497572531315987No Hit
CCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG21178160.5212098004658179RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA21155830.5206602430517461RNA PCR Primer, Index 1 (100% over 23bp)
CGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAAC19442140.47848509549594953RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG18548010.4564799109619531Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA17397780.4281719206176645RNA PCR Primer, Index 1 (100% over 32bp)
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA15763160.3879427428214143RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA15532290.3822608655179307RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGG14825620.36486920686131563Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC14578160.3587790376859354RNA PCR Primer, Index 1 (100% over 33bp)
CGCGGGCCGGTCCCCCCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAAC13482720.3318194687799362RNA PCR Primer, Index 1 (100% over 24bp)
CGCGGGCCGGTCCCCCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACT13344730.328423435301755RNA PCR Primer, Index 1 (100% over 25bp)
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGGAATTCTCGGGTG12978530.3194109890396348No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA12309540.30294666237416307RNA PCR Primer, Index 1 (100% over 23bp)
CGGTCCCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAA12274970.3020958697272993RNA PCR Primer, Index 1 (100% over 23bp)
AGGGGCCCGGGGCGGGGTCCGCCGGCTGGAATTCTCGGGTGCCAAGGAAC12115470.2981704596267855RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA11717350.2883724391301299RNA PCR Primer, Index 1 (100% over 22bp)
CGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGTGGAATTCTCGGGTGCCA10689900.26308615318797984No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA10627070.26153986154775866RNA PCR Primer, Index 1 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA9997790.24605282663834582No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG9878230.24311036875986664No Hit
GAATACAAGCTTGGGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC9810000.2414311792228255RNA PCR Primer, Index 1 (100% over 31bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG9527160.23447028271605855Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGGTCGGCGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA9165110.22555997094871666RNA PCR Primer, Index 5 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA9029100.22221266669936937No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA8786210.2162349685218534RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA8204110.2019090674591004RNA PCR Primer, Index 1 (100% over 23bp)
GTCAGGATGGCCGAGTGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC8167730.2010137294060804RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG7955360.19578715045281314Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGCCGGTCCCCCCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAACTC7728420.19020199328534854RNA PCR Primer, Index 1 (100% over 26bp)
ACGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAA7700000.1895025565765297RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC7404380.18222713504728896RNA PCR Primer, Index 1 (100% over 26bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG7383220.181706372177528Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCA7356470.18104803537383682No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCATGGAATTCTCGGGTGCCAAG7287540.17935161833165236No Hit
CCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC7063240.1738314334692997RNA PCR Primer, Index 5 (100% over 42bp)
GAGACGCGACCTCAGATCAGACGTGGCGTGGAATTCTCGGGTGCCAAGGA6798810.16732362034780207RNA PCR Primer, Index 1 (100% over 22bp)
CCGGCGGCGGGGGCACGGTCCCCCGCGAGGTGGAATTCTCGGGTGCCAAG6692070.16469667192066195No Hit
CCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA6605150.16255750799629418RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG6469730.15922472407271057No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC6258110.15401660316066837No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA5917600.14563640633730807No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTGGAATTCTCGGGTGCCAAGGAAC5858300.14417699054445246RNA PCR Primer, Index 1 (100% over 24bp)
GTCAGGATGGCCGAGCGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC5676810.139710390675222RNA PCR Primer, Index 1 (100% over 24bp)
AGGGAGGACAGAAACCTCCCGTGGAGCTGGAATTCTCGGGTGCCAAGGAA5577420.1372643310520868RNA PCR Primer, Index 1 (100% over 23bp)
AGCGGCCCCCGGCGCGCCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC5237140.12888979469470216RNA PCR Primer, Index 1 (100% over 27bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGA5176010.12738534128125759RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA5009100.12327756573344091RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGAA4876380.12001123075826725RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA4871150.11988251668412502No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG4746760.11682118902015691Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGA4642710.11426044343421041RNA PCR Primer, Index 1 (100% over 22bp)
AGCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG4620770.11372048420157548RNA PCR Primer, Index 5 (100% over 37bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA4564830.1123437604334078No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGG4415010.10865658211830445Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAGGAA4393650.10813089710421686RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG4355230.10718535317906486Illumina Small RNA Adapter 2 (100% over 21bp)
AACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTGCCAAGG4256510.10475578273942394Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCACGCGCGCGCGCGCGCGCGGACTGGAATTCTCGGGTGCCAAGGAAC4169430.10261268109959484RNA PCR Primer, Index 1 (100% over 24bp)
CGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACTGGAATTCTCGGGTGC4067940.10011493896102965No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)