FastQCFastQC Report
Sat 23 Sept 2023
SRR8558083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8558083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406058053
Total Bases30.4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG99502332.450445921829803Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA44027811.0842737799365847RNA PCR Primer, Index 1 (100% over 22bp)
CCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG34005130.8374450339986239RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG30476930.7505559802307381No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA27661060.6812094919836499RNA PCR Primer, Index 1 (100% over 22bp)
CGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAAC24975830.6150802776961549RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA24318590.5988944147353236RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC23082680.5684576338152318RNA PCR Primer, Index 1 (100% over 27bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA23028780.567130237409674No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG21670800.5336872360957707No Hit
CGCGGGCCGGTCCCCCCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAAC20923320.5152790308040018RNA PCR Primer, Index 1 (100% over 24bp)
AGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA20006000.49268817234859763RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA19825760.4882493981716452RNA PCR Primer, Index 1 (100% over 22bp)
AGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC18630570.45881542952677257RNA PCR Primer, Index 1 (100% over 33bp)
CGGTCCCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAA14576470.35897502567200656RNA PCR Primer, Index 1 (100% over 23bp)
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAA12782060.3147840537963669No Hit
GAATACAAGCTTGGGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC12349390.30412868083175293RNA PCR Primer, Index 1 (100% over 31bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA12068210.2972040552043922RNA PCR Primer, Index 1 (100% over 23bp)
ACGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAA12067790.2971937118557774RNA PCR Primer, Index 1 (100% over 23bp)
AGGGGCCCGGGGCGGGGTCCGCCGGCTGGAATTCTCGGGTGCCAAGGAAC12022230.29607170480128364RNA PCR Primer, Index 1 (100% over 24bp)
CGCGGGCCGGTCCCCCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACT11868520.2922862854784954RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG11815070.29096997123216767No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA11695890.2880349229276337RNA PCR Primer, Index 1 (100% over 23bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAGGAA11617630.2861076123024212RNA PCR Primer, Index 1 (100% over 23bp)
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGTGGAATTCTCGGGTGC11377180.28018604522048474No Hit
CGGGCCGGTCCCCCCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAACTC11358850.2797346319345131RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG11087180.2730442092722146Illumina Small RNA Adapter 2 (100% over 21bp)
AACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTGCCAAGG10789620.2657161930488791Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA10544200.25967222967500164No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG10369450.2553686578406561No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA10182300.2507597109519707No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA9663110.23797360817272104RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC9523970.23454700453878202RNA PCR Primer, Index 1 (100% over 26bp)
ACGCACGCGCGCGCGCGCGCGCGGACTGGAATTCTCGGGTGCCAAGGAAC9199260.22655036470856543RNA PCR Primer, Index 1 (100% over 24bp)
GAGACGCGACCTCAGATCAGACGTGGCGTGGAATTCTCGGGTGCCAAGGA8736490.21515371842656203RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA8728300.2149520231285747RNA PCR Primer, Index 1 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG8566430.21096564731841436Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG8219390.20241908612018095Illumina Small RNA Adapter 2 (100% over 21bp)
GCACGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGG7953290.19586583596213025Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGA7672800.18895820297892232RNA PCR Primer, Index 1 (100% over 22bp)
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT7352760.1810765713344934RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGG7326910.1804399628542769Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTC7112850.17516830284363305RNA PCR Primer, Index 1 (100% over 26bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG7005010.172512524951697No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCTGGAATTCTCGGGT6922120.17047119122151727No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA6905250.17005573338549207RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA6893730.16977203010920217RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGAA6757560.1664185687261816RNA PCR Primer, Index 1 (100% over 23bp)
ACGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGG6681810.16455307192245242Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGTGGAATTCTCGGGTGCCA6665610.16415411419016976No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA6388260.1573238100513672RNA PCR Primer, Index 1 (100% over 22bp)
GTCAGGATGGCCGAGCGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC6195720.15258212352212602RNA PCR Primer, Index 1 (100% over 24bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA6158770.15167215511423437No Hit
ATGGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGAAC6124960.1508395155507481RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA5882160.14486007497061018RNA PCR Primer, Index 1 (100% over 23bp)
AGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5819560.14331842348660428RNA PCR Primer, Index 1 (100% over 31bp)
GGGAGAGCGACGCTCAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC5782820.14241362675301997RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA5668820.13960614641473446No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA5643490.13898234398518383No Hit
AGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCAAGGAACTC5522320.13599828790983243RNA PCR Primer, Index 1 (100% over 26bp)
AGGGAGGACAGAAACCTCCCGTGGAGCTGGAATTCTCGGGTGCCAAGGAA5519110.13591923517399124RNA PCR Primer, Index 1 (100% over 23bp)
GTCAGGATGGCCGAGTGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC5442180.13402467848605873RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA5389600.13272978974757582No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG5378190.13244879544354216Illumina Small RNA Adapter 2 (100% over 21bp)
AGCGGCCCCCGGCGCGCCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC5366670.13216509216725225RNA PCR Primer, Index 1 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTGGAATTCTCGGGTGCCAAGGAAC5221950.12860106975886032RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA5217670.12849566611107205No Hit
CCCCGCGAGGGGGGCCCGGGCACTGGAATTCTCGGGTGCCAAGGAACTCC5091290.1253833032588569RNA PCR Primer, Index 1 (100% over 27bp)
CCGGCGGCGGGGGCACGGTCCCCCGCGAGGTGGAATTCTCGGGTGCCAAG4929560.12140037523156817No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG4801540.11824762406571457No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG4777470.11765485168200815Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCC4646570.11443117469708206No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA4626190.11392927601906223No Hit
ATTGCAGTACCTCCAGGAACGGTGCACCTGGAATTCTCGGGTGCCAAGGA4600240.1132902048367946RNA PCR Primer, Index 1 (100% over 22bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAG4444720.10946021060688088No Hit
AGAGCACTGGGCAGAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC4386180.10801854482615075RNA PCR Primer, Index 1 (100% over 31bp)
GACGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGG4224840.10404522133686141Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA4221340.10395902676507195RNA PCR Primer, Index 7 (100% over 37bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCTGGAATTCTCGGGTGCCAA4198650.10340023966967106No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA4136030.10185809564525494RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)