FastQCFastQC Report
Fri 22 Sept 2023
SRR8558084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8558084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386563970
Total Bases28.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA39486051.0214622433642744No Hit
CCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG38370680.9926088041779992RNA PCR Primer, Index 1 (100% over 29bp)
CCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC34651050.8963859202915367RNA PCR Primer, Index 3 (100% over 42bp)
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAA34394440.8897476916951159No Hit
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGTGGAATTCTCGGGTGC32650530.8446345891987813No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG28788670.7447323660298708No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG27996230.7242327835157529Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGTGGAATTCTCGGGTGCCA26931970.6967015058335623No Hit
AGCGGTCGGCGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA23535450.6088371350283888RNA PCR Primer, Index 3 (100% over 36bp)
CGCGGGCCGGTCCCCCCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAAC23111000.5978570636057986RNA PCR Primer, Index 1 (100% over 24bp)
AGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA22117350.5721523917503227RNA PCR Primer, Index 1 (100% over 32bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC22106510.5718719724448194No Hit
CGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAAC19236020.4976154399490465RNA PCR Primer, Index 1 (100% over 24bp)
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCTGGAATTCTCGGGTG17193980.44479003048318244No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA14557830.3765956252984467RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGCCGGTCCCCCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACT12840710.33217555169458757RNA PCR Primer, Index 1 (100% over 25bp)
AGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCAAGGAACTC11665360.3017704935097806RNA PCR Primer, Index 1 (100% over 26bp)
AGCGGTCGGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG11660570.3016465812889908RNA PCR Primer, Index 3 (100% over 37bp)
CGGCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG11468500.29667793405577864RNA PCR Primer, Index 3 (100% over 37bp)
CCGCCGCGGCCCGGGTTCGATTCCCGGTCATGGAATTCTCGGGTGCCAAG11154330.288550689294711No Hit
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT11074220.28647832854158656RNA PCR Primer, Index 1 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG10855510.28082053275684227No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG10571580.2734755647299463No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA9939230.2571173407599265RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC9825830.25418380300678306RNA PCR Primer, Index 1 (100% over 31bp)
ATCCCGGACGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC9295180.24045645019632844RNA PCR Primer, Index 3 (100% over 39bp)
AGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC9008080.23302947763083043RNA PCR Primer, Index 1 (100% over 33bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA8650880.22378909239782488No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCA8313400.21505884265416667No Hit
ATCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT8267830.21387999507558866RNA PCR Primer, Index 3 (100% over 41bp)
CCGCAGCAACCTCGGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA8136430.21048081640924787RNA PCR Primer, Index 1 (100% over 32bp)
AACCCGGCGGCGGGTCCGGCCGTGTCGGCTGGAATTCTCGGGTGCCAAGG7912170.20467944800960108Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCGCGAGGGGGGCCCGGGCACTGGAATTCTCGGGTGCCAAGGAACTCC7688980.19890575937534996RNA PCR Primer, Index 1 (100% over 27bp)
GTCAGGATGGCCGAGTGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC7679720.19866621299444953RNA PCR Primer, Index 1 (100% over 24bp)
ATCCAGCGATCCGAGTTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA7574720.19594997433413155RNA PCR Primer, Index 1 (100% over 32bp)
CGGTCCCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAA7476750.19341559431935676RNA PCR Primer, Index 1 (100% over 23bp)
ATCCGGGTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC7392860.19124544897446083RNA PCR Primer, Index 3 (100% over 39bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA7348540.1900989375704104RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT7159200.18520091254236654RNA PCR Primer, Index 3 (100% over 41bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA7135260.18458161012781402No Hit
CGCCGCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC6903060.17857484234756799RNA PCR Primer, Index 3 (100% over 39bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC6554610.1695608103362556No Hit
CGGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC6412150.16587552119769464RNA PCR Primer, Index 3 (100% over 42bp)
AGAGCACTGGGCAGAAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC6309700.16322524833341298RNA PCR Primer, Index 1 (100% over 31bp)
TCCGCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT6306820.1631507457873014RNA PCR Primer, Index 3 (100% over 41bp)
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC6262090.16199362811800594RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC6226550.16107424600383735RNA PCR Primer, Index 1 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA6208050.16059567062082894RNA PCR Primer, Index 1 (100% over 23bp)
ACGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAA6139170.15881381805966036RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA6137370.1587672539683406RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA6086570.15745311183553914No Hit
GCACGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGG5797270.14996922760287257Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGCGGGAGGGCGCGTGCCCCGCCGCGTGGAATTCTCGGGTGCCAAGGA5738850.1484579641501509RNA PCR Primer, Index 1 (100% over 22bp)
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA5715480.14785340703118297RNA PCR Primer, Index 3 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG5637630.14583951008160434No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC5538480.14327460471807552RNA PCR Primer, Index 1 (100% over 24bp)
AGGGGCCCGGGGCGGGGTCCGCCGGCTGGAATTCTCGGGTGCCAAGGAAC5535570.14319932610377528RNA PCR Primer, Index 1 (100% over 24bp)
AGCCCCCCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG5501940.14232935366428487RNA PCR Primer, Index 3 (100% over 38bp)
CCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAACTCCA5471660.1415460421725284RNA PCR Primer, Index 1 (100% over 28bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA5378960.13914799146956194No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA5309590.13735346312797855RNA PCR Primer, Index 1 (100% over 23bp)
CGGGCCGGTCCCCCCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAACTC5198930.1344908062693996RNA PCR Primer, Index 1 (100% over 26bp)
GCATGGGTGGTTCAGTGGTAGAATTCTGGAATTCTCGGGTGCCAAGGAAC5136690.1328807234673216RNA PCR Primer, Index 1 (100% over 24bp)
ATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG5051690.13068186359944514Illumina Small RNA Adapter 2 (100% over 21bp)
GCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGTGGAATTCTCGGGTGCCAA5016080.129760670659503No Hit
GGGAGAGCGACGCTCAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC4971350.12860355299020754RNA PCR Primer, Index 1 (100% over 31bp)
CTGTCCCCGCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4945310.1279299258024487RNA PCR Primer, Index 1 (100% over 32bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCTGGAATTCTCGGGTGCCAAGGA4900710.12677617109530412RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCATGGAATTCTCGGGTGCCAAGG4758220.12309010588855449Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA4751200.12290850593240751RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA4736530.12252900858815165RNA PCR Primer, Index 3 (100% over 40bp)
GAGACGCGACCTCAGATCAGACGTGGCGTGGAATTCTCGGGTGCCAAGGA4669040.12078311385305776RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA4652790.12036274358419902No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA4575900.11837368081665758No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGCCAAGGA4558080.11791269631259219RNA PCR Primer, Index 1 (100% over 22bp)
GCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGTGGAATTCTCGGGTGCC4396070.11372166940441966No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCTGGAATTCTCGGGTGCCAAGG4385960.11346013442484049Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA4367930.11299371744345445RNA PCR Primer, Index 1 (100% over 23bp)
ATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA4335470.11215401166332185RNA PCR Primer, Index 1 (100% over 22bp)
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGTGGAATTCTCGGGTGCCA4322970.11183064991804591No Hit
AGCCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA4272350.11052116419437642RNA PCR Primer, Index 3 (100% over 40bp)
CCCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG4209810.10890332071041178RNA PCR Primer, Index 3 (100% over 38bp)
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA4156440.10752269540278159RNA PCR Primer, Index 1 (100% over 22bp)
AGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC4109530.10630918344510999RNA PCR Primer, Index 1 (100% over 31bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA4087870.10574886221289585No Hit
AAAGGGCAGGGACTTAATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC4084960.10567358359859559RNA PCR Primer, Index 1 (100% over 31bp)
GTGCCCCGCCGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT3997150.10340203201038109RNA PCR Primer, Index 3 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)