FastQCFastQC Report
Fri 22 Sept 2023
SRR8558085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8558085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444079333
Total Bases33.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG77280881.740249416200596Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA67432081.5184692236060444No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCA41841560.9422091255032577No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA35086940.7901052220324787RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA33303820.7499520361601697RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA27453110.6182028290877477RNA PCR Primer, Index 1 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA24299730.5471934448253191RNA PCR Primer, Index 1 (100% over 22bp)
CGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAAC23817520.5363347994399911RNA PCR Primer, Index 1 (100% over 24bp)
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAA22555530.5079166789326808No Hit
CCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG19641600.44229934924713105RNA PCR Primer, Index 1 (100% over 29bp)
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA19285760.43428636657585684RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC18850640.4244881173067335RNA PCR Primer, Index 1 (100% over 27bp)
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCCATGTGGAATTCTCGGGTGC17722100.39907509048614065No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA17594630.3962046574232267RNA PCR Primer, Index 1 (100% over 23bp)
ACGCGGGAGGCCCGGGTTCGATTCCCGGCCCATGTGGAATTCTCGGGTGC16858750.37963374440575465No Hit
CGCGGGCCGGTCCCCCCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAAC16536480.3723767077446948RNA PCR Primer, Index 1 (100% over 24bp)
ACGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGA16321160.367528024547812RNA PCR Primer, Index 1 (100% over 22bp)
AGGCGGCGGAGGGGCCGCGGGCCGGTCCCCCCCGCCGGGTCTGGAATTCT14208820.3199612984466449No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA14205190.3198795562954064RNA PCR Primer, Index 1 (100% over 23bp)
GAGACGCGACCTCAGATCAGACGTGGCGTGGAATTCTCGGGTGCCAAGGA14186950.3194688188743069RNA PCR Primer, Index 1 (100% over 22bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC14185860.3194442737104363No Hit
AGGGGCCCGGGGCGGGGTCCGCCGGCTGGAATTCTCGGGTGCCAAGGAAC13984400.3149076969091917RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA13823380.3112817682060426RNA PCR Primer, Index 1 (100% over 23bp)
CACGCGGGAGGCCCGGGTTCGATTCCCGGCCCATGTGGAATTCTCGGGTG12375580.27867948540627085No Hit
CACGCGGGAGGCCCGGGTTCGTTTCCCGGCCCATGTGGAATTCTCGGGTG12023680.27075522562091403No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA11698930.263442343082424No Hit
CGGCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC11445100.2577264724904458RNA PCR Primer, Index 4 (100% over 37bp)
CGCGGGCCGGTCCCCCCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACT11269970.25378280776691764RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGA10878540.24496839171752222RNA PCR Primer, Index 1 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG10561920.23783858457560778No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCCATGTGGAATTCTCGGGTGCC10452970.23538519411350317No Hit
CCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGAA10130970.22813423744716352RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG10105150.22755280980391854Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC9847060.2217410104063546RNA PCR Primer, Index 1 (100% over 33bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA9719560.21886990178847163RNA PCR Primer, Index 1 (100% over 22bp)
ACGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAA9379800.21121901658053516RNA PCR Primer, Index 1 (100% over 23bp)
ACGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGG9333160.21016875378886413Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGG9278490.20893766745051384Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCC9156660.2061942387217556No Hit
AGACGCGACCTCAGATCAGACGTGGCGTGGAATTCTCGGGTGCCAAGGAA9144400.20591816192445955RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG8928390.2010539409632918No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG8798850.19813689460752276No Hit
ATGGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAACT8628730.19430604756380318RNA PCR Primer, Index 1 (100% over 25bp)
ATGGGTGGTTCAGTGGTAGAATTCTCTGGAATTCTCGGGTGCCAAGGAAC8580250.19321435073403878RNA PCR Primer, Index 1 (100% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCTGGAATTCTCGGGTGCCAAGGAAC8306870.18705824348731853RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC8305950.18703752646827182RNA PCR Primer, Index 1 (100% over 24bp)
CTCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGGAATTCTCG8124170.18294411372663452No Hit
AGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA7977530.1796420010385847RNA PCR Primer, Index 1 (100% over 32bp)
CCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT7680810.17296031202604964RNA PCR Primer, Index 1 (100% over 30bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGTGGAATTCTCGG7458540.16795512526136858No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAAC7364790.16584401598351348RNA PCR Primer, Index 1 (100% over 24bp)
GTCAGGATGGCCGAGTGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC7223500.16266237726491992RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA6893820.1552384785265384RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGAGGCCCGGGTTCGATTCCCGGCCCATGTGGAATTCTCGGGTGCC6880170.1549311010156827No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCTGGAATTCTCGGGTGCCAAGG6788010.15285579615118003Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGTCCCATTCGGGGCTGATCCGGGTTGAAGACTGGAATTCTCGGGTGC6680400.15043258047768684No Hit
CGGTCCCCCGCGAGGGGGGCCCGGGCATGGAATTCTCGGGTGCCAAGGAA6487390.1460862849926862RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCTGGAATTCTCGGGTGCCAAGGA6343230.14284001818206657RNA PCR Primer, Index 1 (100% over 22bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAG6084190.13700682621048701No Hit
CCGGCGGATCTTTCCCGCCCCCCGTTCCTCCCGACTGGAATTCTCGGGTG5668920.12765556914579496No Hit
CTGTCCCCGCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA5612280.126380121364486RNA PCR Primer, Index 1 (100% over 32bp)
CCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGAA5566050.12533909115739011RNA PCR Primer, Index 1 (100% over 23bp)
CATGGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAAC5491340.12365673409980554RNA PCR Primer, Index 1 (100% over 24bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC5365470.1208223306352336No Hit
CCCGCGAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCTGGAATTCTCGGG5317410.11974009157503396No Hit
ACGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGTGGAATTCTCGGGTGC5123540.11537443018092444No Hit
CGGGCCGGTCCCCCCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAACTC5097730.1147932277226691RNA PCR Primer, Index 1 (100% over 26bp)
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGGAATTCTCGG5034810.11337636376786758No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA4974480.11201782272538226RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGTGGAATTCTCGGGTGCCAA4864780.10954754338905477No Hit
GAATACAAGCTTGGGCTGCAGGTCTGGAATTCTCGGGTGCCAAGGAACTC4822470.10859478569789692RNA PCR Primer, Index 1 (100% over 26bp)
ATTGCAGTACCTCCAGGAACGGTGCACCTGGAATTCTCGGGTGCCAAGGA4785880.10777083382081191RNA PCR Primer, Index 1 (100% over 22bp)
ACGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGTGGAATTCTCGGGTGC4716090.10619926777812919No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTTGGAATTCTCGGGTGCCAAGGAAC4665710.10506478580033357RNA PCR Primer, Index 1 (100% over 24bp)
GACGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGG4580670.10314981264845306Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGGGTGGAATTCT4443890.1000697323601862No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG4442300.10003392794683376Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)