FastQCFastQC Report
Fri 14 Jul 2023
SRR8590755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20846279
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA3726631.787671555196973No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA2615961.2548810269688897No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT1919510.9207926268280301No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1841300.8832751398942709No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1629790.7818133874155672No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA1523470.7308114795930727No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1374090.6591536072217014No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC974130.46729202847184387No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG946540.45405705257998324No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA892680.4282203073267896No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA807740.3874744264911738Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG659260.31624828584516207No Hit
TGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCGG646140.3099545966932516No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA640970.30747453778201855No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG607480.29140932057946645No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG606040.29071854981889095No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC533890.25610805650255375No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATAGATCGGAAG531160.25479847026896263No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA522660.2507210039738987No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATAG505780.24262363561381867No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG489510.23481888542314913No Hit
CCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC485070.23268900891137453No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT481610.23102924027832497No Hit
TCGACTGCATAATTTGTGGTAGTGGCTGTAGGCACCATCAATAGATCGGA451080.21638394075028927No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC436070.20918361497512336No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA417030.20005009047418007No Hit
CCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG361910.17360892080548285No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA338630.16244146017617822No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA324250.1555433466087641No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCCTGTAGGCACCATCAATAGAT316160.1516625580996973No Hit
TCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC312190.1497581414889439No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA311190.14927843957187756No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT309320.14838139698696348No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC297360.14264416205884992No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAG285280.13684936290068841No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG277070.13291101016157367No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG253050.12138857011363995No Hit
TCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG252840.12128783271105602No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC251550.12066901723804042No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA245520.11777641467813033No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT244970.11751257862374384No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTACTGTAGGCACCATCAATAGA244630.11734947997194128No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCAT239870.11506609884670545No Hit
TCGACTGCATAATTTGTGGTAGTGCTGTAGGCACCATCAATAGATCGGAA234420.11245172339869383No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCCTGTAGGCACCATCAATAG232380.11147313148787848No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA231190.11090228620656951No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA228390.10955912083878375No Hit
AGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTACTGTAGGCACCATCA227550.109156171228448No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC227540.10915137420927734No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT222880.10691596327574815No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT208970.10024330960935522No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)