FastQCFastQC Report
Fri 14 Jul 2023
SRR8590756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22365011
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4281651.9144412672097502Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA2533751.132908005276635No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1685380.7535788826573795No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT1514750.6772856047332148No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1335080.5969502988395579No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA1333840.5963958613747161No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA1283680.5739679716678878No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1101820.4926534576710022No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT977990.43728572277473954No Hit
CTGTAGGCACCATCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA859080.38411785265833315No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC777640.34770383077388156No Hit
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG646280.288969229659668No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG646120.28889768934162385No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA605780.2708605866547528No Hit
TGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCGG518530.23184875697132454No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC466780.208709935353933No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC466040.208379061382979No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG447720.20018769496692848No Hit
CCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC440560.19698626573445457No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA430310.19240321410975383No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA429210.1919113744232006No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATAGATCGGAAG422230.18879042804852633No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCCTGTAGGCACCATCAATAG417480.18666657485659185No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG408410.1826111330774664No Hit
ATCCGGCTCGAAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT369960.16541910039749144No Hit
TCGACTGCATAATTTGTGGTAGTGGCTGTAGGCACCATCAATAGATCGGA353970.15826953986295825No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT351330.15708912461523045No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCAT346510.15493397253415167No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC322700.14428787895521267No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC316840.14166771480684717No Hit
CCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG307910.13767487080601032No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT296680.13265363473328942No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC293900.13141062170727302No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG291420.13030174677758935No Hit
CTGTAGGCACCATCAATAACTGTAGGCACCATCAATAGATCGGAAGAGCA287340.12847746866746457No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCCTGTAGGCACCATCAATAGAT286580.12813765215675504No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA285070.12746249040521374No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA284530.12722104183181487No Hit
TCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC260140.11631561459996599No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA242810.10856690390181342No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG241980.1081957885019596No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAGATCGGAAGA240960.1077397189744284No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG232640.10401962243613475No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC223670.10000889335578685No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)