FastQCFastQC Report
Fri 14 Jul 2023
SRR8590758_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590758_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28918795
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5145571.7793168767924115Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA1872510.6475062325383889No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT1707190.5903392586032716No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1454460.5029462672977902No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1406200.4862581584052863No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1402990.48514815364886404No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1246030.4308720332226844No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC1198790.4145366361219408No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1110490.384002860423472No Hit
CTGTAGGCACCATCAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1038420.35908135176448397No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1004090.34721017940062854No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC754290.2608303700067724No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT699480.24187729813776818No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA690450.23875476139306634No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT685560.23706381956786235No Hit
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG629290.21760588572241688No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA604150.20891257744314728No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC556860.19255989054868988No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC555870.1922175526331578No Hit
CCGTCTCCGCCCCCCGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGC548570.18969324275095142No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA542560.1876150095465596No Hit
CCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG541360.1872000544974298No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATAGATCGGAAG511270.17679505664049971No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG504970.1746165426325682No Hit
CCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC504730.17453355162274223No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG503090.1739664463889315No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA492360.1702560566579624No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAGATCGGAAGA466790.16141405615275461No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGCTGTAGGCACCATCAATAGA459570.15891740994049025No Hit
ATCCGGCTCGAAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT450510.15578449931956018No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA422720.14617483197346223No Hit
TCGACTGCATAATTTGTGGTAGTGGCTGTAGGCACCATCAATAGATCGGA417820.1444804321895155No Hit
TCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG398740.13788264690835147No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA378320.13082149515565916No Hit
CTGTAGGCACCATCAATAACTGTAGGCACCATCAATAGATCGGAAGAGCA358830.12408193356604244No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTACTGTAGGCACCATCAATAGA358430.12394361521633249No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT355150.122809404748711No Hit
TCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC345950.11962808270538244No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG334330.11560993464630874No Hit
TCGGGCCCCACGTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA330870.11441348092131778No Hit
ATCTCGCTGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT328860.11371843121402533No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC325530.1125669309526901No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC323800.11196870409019465No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA318430.11011178024533871No Hit
TGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCGG318210.11003570515299825No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA315720.10917467342605389No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCCTGTAGGCACCATCAATAG308310.1066123259976773No Hit
ATCCGGCTCGGAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT307900.1064705496892246No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG305620.10568213509587795No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT302460.10458942013316945No Hit
ATCCGGCTCGAAGGACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC293340.10143576175978286No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)