FastQCFastQC Report
Fri 14 Jul 2023
SRR8590766_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590766_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36836756
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length61
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA3290920.8933794278736162No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3213220.8722863652814596Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA2066360.560950589677332No Hit
AAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTCTGTAGGCACCATCAA2043290.5546878232165721No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1716240.46590421805872373No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA1713800.4652418361703729No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGAT1636230.4441840644165301No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1278850.34716683521209085No Hit
TACTTGGATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCGGAA1235150.3353036841789217No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCATTCTGTAGGCACCATCAAT1168930.31732707407785854No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA1159190.31468297588419564No Hit
TGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCGG1156780.31402873803545567No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG997710.2708463253387459No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG975460.26480616262734974No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA955040.25926278633221667No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA917750.2491397450959037No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGA917290.24901486982187032No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC885450.2403713291148656No Hit
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG811310.2202446925565324No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATAG785350.21319738361325846No Hit
ACCGCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCAC746830.20274043675289974No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA719040.19519634139336264No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT691640.1877581185487669No Hit
AAATTACCCACTCCCGACCCGGGGAGGTAGTCTGTAGGCACCATCAATAG680300.18467967157585755No Hit
GCGCAGCGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATAGATCGGAA636450.17277580034463405No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA613540.1665564687617987No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA581940.15797808037168093No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA580910.15769846834504103No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA574150.1558633447527247No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA573300.15563259696375No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT567990.1541911019526258No Hit
AGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCAATAGATCGG550220.1493671158231197No Hit
AAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATCTGTAGGCACC549540.14918251759193998No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG521680.14161941947331086No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT513380.13936623518096977No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC512950.1392495039465473No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT506740.13756368774709696No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA499970.13572584947491034No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAG498210.1352480658177392No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC488240.13254152998705965No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG483420.13123305428957968No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG476360.12931649030115464No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG447480.1214764948357559No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC442870.12022502741555201No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCCTGTAGGCACCAT416380.11303384043915268No Hit
AACCATCCTCTGCTACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC402450.10925229137983812No Hit
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTCTGTAGGCACCATC399210.10837273510186402No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA397390.10787866336547115No Hit
TGATGAAGAACGCAGCTAGCTGCGAGAATTCTGTAGGCACCATCAATAGA390610.10603811041341425No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA384250.10431157401590956No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT380840.10338586818014052No Hit
GAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGA378430.10273163033140051No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT378040.1026257578164592No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT375690.10198780804694095No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCCTGTAGGCACCATCAAT370370.10054359835594644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)