FastQCFastQC Report
Fri 14 Jul 2023
SRR8590769_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590769_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42868922
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length61
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA4544341.0600546475136463No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA4010750.9355845243787563No Hit
AAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTCTGTAGGCACCATCAA3443750.8033208766014691No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA2729610.6367339957837055No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA2491630.5812205867924555No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT2143130.4999262635995372No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT1686200.3933385588748884No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA1604860.3743644405147393No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1558610.3635757390866978No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA1545350.3604825892286258No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG1395990.32564149851960356No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG1363400.31803925463766036No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG1263790.29480330762690976No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1247850.29108499625906153No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT1203670.2807791621165561No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1197300.27929323718473725No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1134710.2646929167008212Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC1063110.24799084054411258No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATAG1016220.23705284681522898No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG984500.229653547154743No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA971720.22667236652230255No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGAT967780.22575328579524348No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG942110.21976526491615536No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT864140.20157726382762786No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT845450.19721746210459878No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG755730.17628854768029856No Hit
TGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCGG714770.16673384042640493No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAG709620.16553250394306626No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC681050.15886800232578743No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCG674450.1573284254733534No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA663920.1548721005860609No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA656370.15311091797456441No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA645600.15059860847445616No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA637480.14870446240752216No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA617120.14395510108698326No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTACTGTAGGCACCATCAATAGA616760.14387112416775957No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT602360.1405120473988126No Hit
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG582000.1357626860782737No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG565220.13184842856557016No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT563600.13147053242906365No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA562620.1312419285933992No Hit
AGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCAATAGATCGG533360.124416471214275No Hit
TACTTGGATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCGGAA524620.12237769823090024No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA496620.11584616006905887No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG488330.11391235823471373No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA487500.11371874478205914No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGA477830.11146303142402321No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCATTCTGTAGGCACCATCAAT458990.10706823931798425No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC453920.10588556437225083No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGG450360.1050551259488167No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)