FastQCFastQC Report
Tue 11 Jul 2023
SRR8590780_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590780_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9574511
Total Bases584 Mbp
Sequences flagged as poor quality0
Sequence length61
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA775360.8098168146655218No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG497860.519984780423773No Hit
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG458500.47887563135078126No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA442020.461663264055992No Hit
TGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCGG439990.4595430513370344No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA421580.44031491529959077No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA391400.4087937232512449No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT386230.40339396967636265No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT330180.34485312095834453No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG316750.3308262949408069No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA284830.2974877777047831No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA256200.26758546728913885Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAG228700.23886337380572228No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA222530.23241918046780666No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG218340.22804297786069702No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC209680.2189981295128284No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT208300.2175568026398424No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA194020.20264220282372644No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC192570.20112776516732814No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA183060.19119514302088117No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA182820.19094447747775317No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC175930.18374828751045352No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA154670.16154349814836497No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG154650.16152260935310428No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC152190.15895328753604232No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG145580.15204954070239202No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC140810.14706756303272303No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG136620.14269136042561337No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT136240.14229447331566072No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTACTGTAGGCACCATCAATAGA135790.14182447542229573No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA134990.14098892361186904No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG132540.1384300461924374No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT129800.1355682812417261No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA128770.13449250828580175No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT121930.12734854030665377No Hit
TGGCCGCCGGTGAAATACCACTACCTGTAGGCACCATCAATAGATCGGAA110400.1153061498388795No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC100050.10449619829148454No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA99180.10358753569764555No Hit
TTGACCTGGTATTGCAGTACTTCCAGGCTGTAGGCACCATCAATAGATCG97130.10144643418342723No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)