FastQCFastQC Report
Fri 14 Jul 2023
SRR8590783_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590783_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8843129
Total Bases539.4 Mbp
Sequences flagged as poor quality0
Sequence length61
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA873490.9877612324777802No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA606870.6862616162220408No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA581920.6580476209269366No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG581360.657414360912297No Hit
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG535040.6050347111299632No Hit
TGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCGG532500.6021624246349906No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA531140.6006245074565801No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT438110.4954241875245742No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG421410.4765394692308571No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA391850.4431123870295231Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT381000.43084297424588064No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA355440.40193917786340105No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA277720.3140517344030603No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAG269060.3042588206052405No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA267860.3029018348595842No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC253440.2865953894826141No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA243670.2755472638700623No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT239630.27097874519301934No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG238920.2701758619601727No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC237770.26887541728725206No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC226200.25579181305621573No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG202380.22885564600493785No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA201900.22831285170667534No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT198870.2248864626988931No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC196450.2221498747784862No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTACTGTAGGCACCATCAATAGA195260.22080419724737704No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG192100.21723080145048204No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA187820.21239088562430786No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG184420.2085460926782816No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC179060.20248488968101674No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG167130.18899418972628354No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT164350.18585050608217973No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA157930.17859063234291844No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT156420.17688309194630092No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA145840.16491900095543105No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC145420.16444405594445133No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA138500.15661877147783324No Hit
AGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTACTGTAGGCACCATCA137820.15584981288862798No Hit
TGGCCGCCGGTGAAATACCACTACCTGTAGGCACCATCAATAGATCGGAA133790.1512926024261322No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC129700.1466675426763536No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA126360.14289059901761017No Hit
AAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAGATC114000.1289136458373501No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA113820.12871009797550165No Hit
TGACCTGGTATTGCAGTACTTCCAGGCTGTAGGCACCATCAATAGATCGG113470.1283143104663519No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA112240.1269234000770542No Hit
TTGACCTGGTATTGCAGTACTTCCAGGCTGTAGGCACCATCAATAGATCG110660.12513670217860667No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG110100.12450344216396708No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATAG105850.11969745098143428No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG104900.11862317059945636No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA103210.11671208234099038No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA103120.11661030841006617No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT102860.11631629483184064No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG102500.11590919910814373No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA102430.11583004160631379No Hit
TCACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAAT95460.10794821606696No Hit
ATCCGGCTCGAAGGACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC95220.10767681891782875No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)