FastQCFastQC Report
Fri 14 Jul 2023
SRR8590790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60103476
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT14057702.3389163049404993No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT4913450.8174984754625506No Hit
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAAT4835840.8045857447579238No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4392420.7308096456850516No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT4201950.6991192988571908No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT4109260.6836975618514975No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC4043540.6727630861150193No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA3509550.5839179750602111No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT3095980.5151083108737339No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT2810510.4676118898680669No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAAT2258700.3758018920569586No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT1895620.3153927403466648No Hit
TGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAAT1884620.31356256333660304No Hit
TACTTGGATAACTGTGGTAATTCTCTGTAGGCACCATCAAT1695310.2820652169934398No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT1608650.2676467497487167No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAAT1529240.2544345355333525No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAAT1351560.22487218542900914No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTACTGTAGGCACCATCAAT1312020.21829353097647797No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC1280380.21302927637662752No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAAT1230840.20478682464222203No Hit
CCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT1205540.20057741751907995No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT1188030.19766410847851795No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT1170630.19476910120805657No Hit
TCCCCGGCATCTCCACCACTGTAGGCACCATCAAT1156480.1924148280542044No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC1105210.1838845393900346No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT1095680.18229894058040835No Hit
ATCCGGCTCGAAGGACCACTGTAGGCACCATCAAT1074500.1787750179373985No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAAT1071410.17826090457729932No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAAT964850.1605314807416463No Hit
TCCCCGGCACCTCCACCACTGTAGGCACCATCAAT900950.1498998161104692No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGAN900550.14983326421919424No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT878280.14612798767246007No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAAT835990.13909178896741348No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT819170.13629328193930082No Hit
GCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT805850.13407710395984418No Hit
TCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAAT784700.13055817270867995No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC776880.12925708323425422No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC761180.12664492150171147No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA752660.12522736621755454No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT746260.12416253595715496No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC695640.11574039411630702No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC689980.11479868485476613No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT688140.11449254615490126No Hit
TTGACCTGGTATTGCAGTACTTCCAGGCTGTAGGCACCATCAAT687090.11431784744030446No Hit
AGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTACTGTAGGCACCATCA681820.11344102627275666No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAAT637430.10605543013851644No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT633170.10534665249643797No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAAT633090.10533334211818299No Hit
TCACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAAT615380.10238675713198352No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)