FastQCFastQC Report
Tue 11 Jul 2023
SRR8590791_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590791_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67052659
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9193051.371019455022656No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT8143331.2144678706924954No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT6357410.948121982753883No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT6089560.9081757667507264No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAAT5109160.7619623257595198No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT5051550.7533705710313442No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT4846170.7227409132276171No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAAT3515230.5242491576657684No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT3095180.46160436381799563No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAAT2879670.4294639530999062No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAAT2860800.42664974702942055No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAAT2696720.40217942736618395No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT2369760.35341775185977337No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT2260470.33711862194756514No Hit
TGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAAT1897780.28302829869878837No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAAT1886220.28130428056551793No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT1827550.27255444112962024No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAAT1693020.25249110553542703No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1686760.2515575109407667No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT1628330.24284346426888154No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAAT1621680.24185170643270087No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT1617110.24117015255129554No Hit
AGTCCGCCCGGAGGATTCCTGTAGGCACCATCAAT1547460.23078279416182435No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAAT1439390.21466561080001317No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT1233360.1839390142604188No Hit
TCGACTGCATAATTTGTGGTAGTCTGTAGGCACCATCAAT1230680.18353932839561216No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT1223400.1824536145538986No Hit
CCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT1220890.18207928189693418No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1010340.1506785883017704No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT1002460.1495033925500255No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT1002440.14950040981969112No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAAT976530.1456362826715045No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC967790.14433282951538132No Hit
AAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT942870.1406163475187464No Hit
ATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAAT924210.1378334601167718No Hit
ACATATACTAAAATTGGAACGATACAGAGAAGACTGTAGGCACCATCAAT876790.13076140649396173No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT861180.12843338546797972No Hit
ACCACCGGCCCGTCTCGCTGTAGGCACCATCAATN835580.12461549063997597No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTACTGTAGGCACCATCAAT835360.1245826806062978No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA823650.12283629199551951No Hit
ACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAAT733870.10944681552449696No Hit
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAAT733370.10937224726613749No Hit
CACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAAT729590.10880851123294007No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)