FastQCFastQC Report
Fri 14 Jul 2023
SRR8590794_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590794_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34419133
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA15740984.5733226342453195Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA3934791.1431984646446498No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2907310.8446784525339439No Hit
TGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCGG2505500.727938149981872No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG2176030.6322152275015178No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2085730.6059798194219477Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG1362870.39596290818830326No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1258760.3657151968354345No Hit
TCAGCCATTGATGATCGTTCTTCTCTCCGCTGTAGGCACCATCAATAGAT1184400.34411093388087377No Hit
TACTTGGATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCGGAA1157290.3362345007353904No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA1144840.3326173265317287No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTACTGTAGGCACCATCAATAGA993010.2885052334118933No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA983100.28562602085299477No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT957370.2781505274987607No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG906580.26339419996430474No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC885320.25721740289042144No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT815300.23687406652573148No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG796820.231504959755959No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA690050.2004844224286533No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG682660.19833736079290548No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA648420.18838940539263438No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA598440.17386841208347695No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG591790.17193634714738457No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA577630.16782235624587055No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT568350.1651261814177597No Hit
ATCCGGCTCGAAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT566570.16460902719426432No Hit
TGACCTGGTATTGCAGTACTTCCAGGCTGTAGGCACCATCAATAGATCGG564180.16391464596159352No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG562090.16330742555310732No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAG553180.16071874907482417No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA537480.15615733260916248No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA537170.15606726642417168Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCCGGCTCGGAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT528410.15352217035798083No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA525540.15268833180661465No Hit
AGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTACTGTAGGCACCATCA525490.15267380500258387No Hit
TCAAGTGATGTCATCTTACTACTGAGACTGTAGGCACCATCAATAGATCG524390.15235421531390694No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT523840.1521944204695685No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCG521050.15138382480465154No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA513970.14932682935389455No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC512600.14892879492345146No Hit
AAATGGATTTTTGGAGCAGGGAGACTGTAGGCACCATCAATAGATCGGAA477350.13868739808175878No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC476900.13855665684548182No Hit
ATCCGGCTCGAAGGACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC476600.1384694960212972No Hit
TGACCTGGTATTGCAGTACCTCCAGCTGTAGGCACCATCAATAGATCGGA466180.13544211006128482No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGG459500.13350132904277398No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT458120.13310038925152473No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA449780.13067731833919233No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA432330.12560746373245368No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT429870.12489274497413981No Hit
TTCCGGCTCGAAGGACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC422990.12289385673950591No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG411550.11957012397726578No Hit
CAGCCATTGATGATCGTTCTTCTCTCCGCTGTAGGCACCATCAATAGATC406770.11818136151192418No Hit
TCAGCCATTGATGATCGTTCTTCTCTCCGTCTGTAGGCACCATCAATAGA403840.11733009079572108No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG399710.11613017678277951No Hit
TCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATAGA392040.11390176504445942No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG390480.11344852875869942No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG384050.11158038176034242No Hit
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTCTGTAGGCACCATC376140.10928224136267466No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGA359820.1045406925270314No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATC359710.1045087335581637No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG354180.10290206903236059No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG353370.10266673480706212No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)