FastQCFastQC Report
Fri 14 Jul 2023
SRR8590795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34223438
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA15849124.6310718402984525Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA3987171.1650407536495895No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2950110.8620145059651809No Hit
TGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCGG2524880.7377634006262024No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG2199990.642831383568185No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2097680.6129366663863519Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG1364540.39871505603849616No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1285170.3755233474789996No Hit
TCAGCCATTGATGATCGTTCTTCTCTCCGCTGTAGGCACCATCAATAGAT1181690.34528675932558267No Hit
TACTTGGATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCGGAA1162610.3397116327120613No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA1162590.339705788763829No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTACTGTAGGCACCATCAATAGA998200.2916714562692386No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA996450.2911601107989209No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT966880.28251983333760916No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG922280.26948782877979705No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC885870.258848921023072No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT824450.24090215600197734No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGG809580.23655718049133465No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATA698820.20419339518139584No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG695670.20327297333482394No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGGA657730.19218700353833534No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA612550.17898552448178937No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCG590580.17256594734871464No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA576910.16857160873200408No Hit
ATCCGGCTCGAAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT575340.16811285879577614No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT574990.1680105897017126No Hit
TGACCTGGTATTGCAGTACTTCCAGGCTGTAGGCACCATCAATAGATCGG569690.1664619434201789No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCG569550.16642103578255346No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAG562130.16425293098840624No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA549920.1606852005926465No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA545730.159460893438Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCCGGCTCGGAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT538910.15746810709081888No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA532750.15566817103530042No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGAT527730.15420134002901753No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCG527690.15418965213255315No Hit
AGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTACTGTAGGCACCATCA523790.1530500822272736No Hit
TCAAGTGATGTCATCTTACTACTGAGACTGTAGGCACCATCAATAGATCG522210.1525884103169296No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC520730.15215595814774657No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA518080.1513816350069797No Hit
ATCCGGCTCGAAGGACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC485590.14188814110376635No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC478450.13980185158487No Hit
AAATGGATTTTTGGAGCAGGGAGACTGTAGGCACCATCAATAGATCGGAA477780.1396060793190912No Hit
TGACCTGGTATTGCAGTACCTCCAGCTGTAGGCACCATCAATAGATCGGA465330.135968221544545No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAGATCGG464440.13570816584821196No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT464260.13565557031412215No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA460010.13441373131477907No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA445910.13029374781107614No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAAT438590.12815486275808993No Hit
TTCCGGCTCGAAGGACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC431840.1261825302297215No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG421550.1231758188642532No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG413670.12087330326076534No Hit
CAGCCATTGATGATCGTTCTTCTCTCCGCTGTAGGCACCATCAATAGATC405800.11857370963139355No Hit
TCAGCCATTGATGATCGTTCTTCTCTCCGTCTGTAGGCACCATCAATAGA403690.11795717309289616No Hit
TCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATAGA397660.11619522270088704No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG388730.1135858998152085No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG385060.11251353531459932No Hit
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTCTGTAGGCACCATC381580.11149668832219604No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCTGTAGGCACCATCAATAGATCGGA366240.10701438002809653No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATC364710.10656731798833304No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG361720.10569364772761872No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG354460.10357229451932913No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA347250.10146555118162003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)