FastQCFastQC Report
Fri 14 Jul 2023
SRR8590797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43309700
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA6612251.5267365047552857No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA6390731.4755886094800934No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA5344491.234016859964396No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3499080.8079206274806799Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG3074000.7097717139578431No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG3019180.6971140414272091No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA2864190.6613276009762247No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2529600.5840723902497593No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA2514880.5806736135323034No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG2389520.5517285965961436No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG1900220.43875159606277575No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1871230.4320579454487101No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT1830960.4227597974587679No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATAG1765420.4076269288404214No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA1571390.3628263414431409No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1405040.3244169320036851No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT1392540.32153074253573677No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA1389660.3208657644823215No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG1352310.312241830352092No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC1336790.30865833750868743No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTACTGTAGGCACCATCAATAGA1326350.30624779206505703No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA1082520.2499486258274705No Hit
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG985070.22744789273534566No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG952400.21990454794191602No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT943640.21788190636277788No Hit
TGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCGG935270.21594931389503969No Hit
TCAAGTGATGTCATCTTACTACTGAGACTGTAGGCACCATCAATAGATCG890190.20554056019783098No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT888100.20505798931879002No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAG860950.19878918579440633No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT750150.1732060023505127No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC697300.16100319328002735No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT690230.15937076451695578No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA684140.15796461300817138No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC680020.15701332495953563No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA633020.14616125256005005No Hit
GCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA592990.13691851940789246No Hit
TCAGCCATTGATGATCGTTCTTCTCTCCGCTGTAGGCACCATCAATAGAT581060.1341639401796826No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG580060.13393304502224676No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACTGTAGGCACCATCAAT565130.13048578032172933No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAATAGATCGG552470.1275626476285913No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT528630.1220581070753203No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC520790.12024788904102313No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATC520050.1200770266245206No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA501390.1157685229867674No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT493930.11404604511229587No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCA466090.10761792392928143No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG448280.10350568117534871No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG441560.10195406571737971No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)