FastQCFastQC Report
Tue 11 Jul 2023
SRR8590798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8482375
Total Bases517.4 Mbp
Sequences flagged as poor quality0
Sequence length61
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1240161.4620433546029268Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA919211.0836705521743615No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGAT733120.8642862405870998No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA698190.823106736026172No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA621070.7321888032538056No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG336220.39637483605711843No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGA334670.39454751764688545No Hit
TATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTCTGTAGGCACCATC297460.3506800866502601No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA289690.34151991629702766No Hit
ACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCATCAATAGATCGG265410.3128958575870555No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT248710.29320797536067433No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA248690.2931843970586068No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA246050.29007206118569384No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCG233120.27482868889904066No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT217850.2568266552704873No Hit
GCGCAGCGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATAGATCGGAA215300.25382042175687825No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG205830.24265609572790642No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC204770.2414064457183277No Hit
ACTTGGATGGGAGACCGCCTGGGAATCTGTAGGCACCATCAATAGATCGG202900.23920187447501437No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA195540.23052505931416611No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA182250.21485727759029752No Hit
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG166440.19621862980592109No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC164870.1943677330936206Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA146870.17314726123285046No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGATC134890.15902385829440457No Hit
TATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTCTGTAGGCACCATCA125130.14751764688545366No Hit
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTCTGTAGGCACCATCAA124210.14643304499034762No Hit
TGATGAAGAACGCAGCTAGCTGCGAGAATTCTGTAGGCACCATCAATAGA121720.1434975463829411No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA121170.14284914307608423No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCCTGTAGGCACCATCAAT118730.1399725902238465No Hit
ACTCCGGATGTGCTGACCCCTGCGACTGTAGGCACCATCAATAGATCGGA114490.13497399018553177No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT111690.13167302789607865No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT109330.128890788252111No Hit
TATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTCTGTAGGCAC106160.1251536273744087No Hit
TACTTGGATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCGGAA103480.12199413489736069No Hit
ACCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG103300.12178193017875301No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT102390.12070911743468074No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGATC101570.11974240704991232No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC101180.11928263015959563No Hit
AACCATCCTCTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT99540.1173492093900588No Hit
ATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTCTGTAGGCACCATCA99410.11719595042661991No Hit
AGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCAATAGATCGG98760.11642965560942542No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC98150.115710517396366No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT98050.11559262588602837No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATAG97960.11548652352672453No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA97490.11493243342813775No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC97420.11484990937090143No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA97370.11479096361573261No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG96970.11431939757438217No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC93860.11065297160288246No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA93090.10974520697328283No Hit
AAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTCTGTAGGCACCATCAA92100.10857808102094048No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA91780.10820082818786012No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT88460.10428683004465142No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA88300.10409820362811122No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG87830.10354411352952446No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAAT86980.10254203569165475No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)