FastQCFastQC Report
Tue 11 Jul 2023
SRR8590799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8937094
Total Bases545.1 Mbp
Sequences flagged as poor quality0
Sequence length61
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA765320.8563409985393462Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA583260.6526282480636323No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA532700.596055048766411No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA494380.553177576514245No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGAT483010.540455320264059No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG287200.32135725550162053No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGA254930.2852493215356132No Hit
TATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTCTGTAGGCACCATC227450.2545010716011267No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT212710.23800801468575805No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA205250.22966078235274243No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA191810.21462233696993677No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT186390.208557725811097No Hit
GCGCAGCGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATAGATCGGAA180940.20245954669381344No Hit
ACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCATCAATAGATCGG166790.18662665962783875No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA166290.18606719365377605No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC155300.1737701315438777No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA154070.17239384524768342No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG150780.1687125591383508No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA141740.158597414327297No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCG141080.15785891924153422No Hit
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG141020.1577917833246467No Hit
ACTTGGATGGGAGACCGCCTGGGAATCTGTAGGCACCATCAATAGATCGG140350.15704209891940268No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA122580.13715867820121394No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGATC104320.1167269808284438No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC100040.11193795209046699Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCCTGTAGGCACCATCAAT97620.10923013677600349No Hit
TATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTCTGTAGGCACCATCA96450.10792098639669674No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA95550.10691394764338386No Hit
TGATGAAGAACGCAGCTAGCTGCGAGAATTCTGTAGGCACCATCAATAGA94260.10547052543030207No Hit
CGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA93760.10491105945623935No Hit
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTCTGTAGGCACCATCAA90190.1009164724014316No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)