FastQCFastQC Report
Fri 14 Jul 2023
SRR8590800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8425713
Total Bases513.9 Mbp
Sequences flagged as poor quality0
Sequence length61
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1223031.4515448128840847Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA924201.0968804657837266No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGAT743900.8828926406584225No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA692510.8219007697034066No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA623410.7398899060530545No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGA340670.4043218656984875No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG334880.39745004369363157No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA292040.3466056819167707No Hit
TATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTCTGTAGGCACCATC291990.34654633975783417No Hit
ACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCATCAATAGATCGG263670.31293494093615576No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT250430.29722113724974963No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA248730.295203503845906No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA245120.290918999970685No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCG232720.2762021445544134No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT217860.2585656549184621No Hit
GCGCAGCGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATAGATCGGAA214370.25442357222468887No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC207290.2460207225192693No Hit
ACTTGGATGGGAGACCGCCTGGGAATCTGTAGGCACCATCAATAGATCGG204760.24301800927707837No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG202780.24066805978318986No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA198900.23606310824971136No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA181710.21566127400731547No Hit
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG167180.19841644262034558No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC164390.19510515015168448Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA149070.1769227126535167No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGATC139130.1651254914569248No Hit
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTCTGTAGGCACCATCAA127920.15182097942334377No Hit
TGATGAAGAACGCAGCTAGCTGCGAGAATTCTGTAGGCACCATCAATAGA122800.14574434234823805No Hit
TATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTCTGTAGGCACCATCA122460.14534081566746931No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCCTGTAGGCACCATCAAT122230.14506784173636106No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA119090.1413411541551439No Hit
ACTCCGGATGTGCTGACCCCTGCGACTGTAGGCACCATCAATAGATCGGA112770.13384030526556032No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT109700.13019669670685435No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT107360.12741948366862246No Hit
TATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTCTGTAGGCAC106180.12601900871771918No Hit
TACTTGGATAACTGTGGTAATTCTCTGTAGGCACCATCAATAGATCGGAA105560.1252831659469056No Hit
ACCTGCGGAAGGATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG102990.12223297897756547No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT102830.12204308406896841No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC101110.1200017138015501No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGATC100770.11959818712078137No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC100430.11919466044001262No Hit
AGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCAATAGATCGG99840.11849442296456099No Hit
AACCATCCTCTGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT99830.11848255453277365No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA97830.11610886817531052No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA97710.11596644699386272No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT97570.11580028894884029No Hit
ATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTCTGTAGGCACCATCA97190.1153492885409223No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATAG96790.11487455126942966No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC96070.11402002418074292No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG95930.1138538661357205No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA95440.11327231297814201No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA93530.11100544250676472No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC93360.11080367916638034No Hit
AAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTCTGTAGGCACCATCAA91810.10896407223934639No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAATAGAT88510.10504748974953218No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA88350.10485759484093513No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAAT86720.10292304045960265No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG86310.10243643475632269No Hit
TGCGCCCGATCTCGTCTGATCTCGGAAGCTCTGTAGGCACCATCAATAGA85010.10089353862397166No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)