FastQCFastQC Report
Fri 14 Jul 2023
SRR8590801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8857645
Total Bases540.3 Mbp
Sequences flagged as poor quality0
Sequence length61
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA747480.8438811896390067Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA563790.6365010112733125No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA531650.6002159716267699No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA479150.5409451383522369No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGAT463870.5236945034487158No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG290340.3277846425319597No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGA250850.2832016862269825No Hit
TATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTCTGTAGGCACCATC234400.26463015846762883No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT212430.23982672595255286No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA201640.22764515850432027No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA188450.21275406724925194No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT187790.21200894820237207No Hit
GCGCAGCGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATAGATCGGAA178530.2015547021809973No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC161980.18287027759635885No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA160260.18092845220145987No Hit
ACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCATCAATAGATCGG159980.18061234109066235No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA154720.17467396808068059No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG147770.1668276387233853No Hit
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG140360.15846198396977978No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA139940.1579878173035835No Hit
ACTTGGATGGGAGACCGCCTGGGAATCTGTAGGCACCATCAATAGATCGG138680.1565653173049947No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCG137900.1556847220677731No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA121790.13749704351438785No Hit
CGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA107910.1218269641648542No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGATC107490.12135279749865793No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA99370.11218557528553019No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC98850.1115985117940491Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGAT97800.11041309512855844No Hit
TATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTCTGTAGGCACCATCA97590.11017601179546031No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCCTGTAGGCACCATCAAT95980.10835837290837462No Hit
TGATGAAGAACGCAGCTAGCTGCGAGAATTCTGTAGGCACCATCAATAGA93550.1056149800539534No Hit
CTGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA92230.10412474196019371No Hit
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTCTGTAGGCACCATCAA89900.10149424593105728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)