FastQCFastQC Report
Fri 14 Jul 2023
SRR8590802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54115105
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA796190614.712908715597983Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC14967822.765922749295229Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA14888332.751233689743372No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG8817731.6294396915611637No Hit
TACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT3164080.5846944212711035Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTACTGTAGGCACCATCAATAGA2707020.5002337147825916No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2653310.4903085746576672Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG2643570.48850870750412484No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG2638420.4875570323664714No Hit
AACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT1888130.3489099762441559Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAATAG1835170.33912342958588No Hit
ATCCGAGTCACGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1793860.3314897014428781No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1712310.31641997183595966No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1557600.28783091153569784No Hit
ACGGAGTCGAGCTCAACCCGCCTGTAGGCACCATCAATAGATCGGAAGAG1420260.2624516759230163No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1336700.24701051582547978Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGAT1328310.24546011691190472No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1269760.234640586948875No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1261420.23309942759974317No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1255570.23201839855988451Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCAAGTGATGTCATCTTACTACTGAGACTGTAGGCACCATCAATAGATCG1152160.2129091313783832No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1096050.20254049216018336No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1093250.20202307655136212Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCCGGACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1092580.2018992663878228Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGA1090820.20157403371942084No Hit
TGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCGG1040920.192352948405071No Hit
TCAGCCATTGATGATCGTTCTTCTCTCCGCTGTAGGCACCATCAATAGAT955440.17655698903291417No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC899480.1662160685080441No Hit
CAGCCATTGATGATCGTTCTTCTCTCCGCTGTAGGCACCATCAATAGATC832800.15389418536654415No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG802650.14832272800727264No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT755890.13968188733995804No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAG718180.13271340783686922No Hit
AGAGCACTGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA688190.12717151708381605Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATAGATC686230.1268093261576412No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA670440.12389147170646718No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG644990.11918853340485988No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG642200.11867296570892731No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAATA625480.11558325535910906No Hit
ATCCGGCTCGGAGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT625160.11552412214667236No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA613480.11336575989273234No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA599840.11084520671261748No Hit
ATCCGGCTCGAAGGACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC575370.10632336387409763No Hit
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAATAGATCG557670.10305255806119197No Hit
TCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATAGA543960.10051906949085658No Hit
ACAAAGCACCCAACTTACACTTAGGAGATTCTGTAGGCACCATCAATAGA542810.10030655950866213No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)