FastQCFastQC Report
Fri 14 Jul 2023
SRR8590812_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590812_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73172239
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21436612.929609684350372No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT17917772.448711457360216No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAAT17318372.3667951448089486No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT8195521.1200313277279927No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT6306940.8619307111813265No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAAT6066950.8291327534749893No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT5638590.7705914260734867No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT5361190.7326808736848958No Hit
CACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAAT5113790.6988702368394112No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT4433580.6059101184535299No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAAT4305870.5884567779865257No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAAT3980980.5440560592931972No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAAT3814570.5213138277755858No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT3399210.46454913044276264No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2855830.3902887268489898No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT2779040.37979430969715167No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2534460.3463690649127191No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT2505910.34246731195419616No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT2312250.3160009904849297No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT2213260.30247263583119277No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC2164630.2958266727358172No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT2137240.292083449844961No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAAT2130310.2911363693545034No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT2084450.284868965127608No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2000670.2734192676542261No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1917980.2621185337789103No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAAT1913370.2614885134237863No Hit
TACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAAT1793920.2451640163696508No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAAT1761910.24078940648515618No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCCTGTAGGCACCATC1705670.23310343148034599No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAAT1703770.23284377016261593No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT1628670.22258031491970612No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT1569760.2145294474315594No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1516870.20730129632906275No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGCTGTAGGCACCATCAAT1498230.20475388213827922No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT1497260.20462131820238547No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1427030.19502341591597327No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAAT1283180.17536432088677784No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT1281010.1750677603291598No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAAT1273950.1741029135380154No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT1243680.16996609875502103No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1237120.16906958388959506No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT1194020.16317937189266546No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAAT1087680.14864653793086746No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAAT1063510.14534337264163805No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAAT1050480.14356264265741547No Hit
TAGCGTTTTCTCCTGAGCGTGAAGCTGTAGGCACCATCAAT1019930.13938756199601873No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGACTGTAGGCACCATCAAT1005510.1374168692582989No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA999330.13657228665641896No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT950350.12987849121304051No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAAT908480.12415637575337826No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT865150.1182347310706182No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT851110.11631597059644438No Hit
CTGACGCGGCAGACAGCCCTCGCTGCTGTAGGCACCATCAAT847690.11584858022453025No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA830960.1135621939899912No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCGCTGTAGGCACCAT819120.11194409398897853No Hit
CTGTAGGCACCATCAATCTGTAGGCACCATCAATN816220.11154776881981156No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAAT791240.10813390581091825No Hit
ACGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT754990.1031798411963313No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT754700.10314020867941462No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC749030.1023653246417675No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATC747550.10216306214164089No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)