FastQCFastQC Report
Fri 14 Jul 2023
SRR8590813_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590813_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82392040
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25569623.1034090186382084No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT22884332.7774928257632654No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA14835161.8005574324898375No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT9939551.2063726058973658No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT9584921.163330826618688No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT9241671.121670248727911No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAAT7879970.9563994288768674No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT6565880.7969070798586855No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAAT5941470.7211218462366025No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAAT5874350.7129754282088415No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT5136460.6234170194110985No Hit
ACATATACTAAAATTGGAACGATACAGAGAACTGTAGGCACCATCAAT4830780.5863163480355627No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT4498620.5460017739577755No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT4450530.5401650450698878No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT4264840.5176276737412983No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAAT3573420.43370937289573125No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3306590.4013239628488383No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT3221870.39104141613680155No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT3195240.3878093077923547No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT2973620.3609110783031953No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAAT2788050.33838827148836226No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA2700150.3277197651617802No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2349260.28513191322851095No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2061630.2502219874638375No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAAT2027250.2460492542726215No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1932560.23455663920932168No Hit
TTGACCTGGTATTGCAGTACCTCCAGGCTGTAGGCACCATCAAT1896160.23013873670320578No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT1878220.2279613418966201No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGCTGTAGGCACCATCAAT1875170.22759116050531095No Hit
TCGACTGCATAATTTGTGGTAGTGGGCTGTAGGCACCATCAAT1840240.22335167329271127No Hit
TTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAAT1831780.22232487507288326No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC1764450.2141529691460486No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1675860.2034007168653671No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA1444490.17531912063349808No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1414130.1716342986531223No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT1345750.1633349532309189No Hit
CCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT1337400.1623215058153676No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAAT1324450.1607497520391533No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT1269710.15410590634726365No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTACTGTAGGCACCATCAAT1192330.1447142223933283No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1129330.13706785267120467No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC1072860.1302140352393265No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAAT1065880.12936686602249442No Hit
TAGCGTTTTCTCCTGAGCGTGAAGCTGTAGGCACCATCAAT1045180.12685448739951094No Hit
CTGTAGGCACCATCAATCTGTAGGCACCATCAATN1020800.12389546368799705No Hit
GCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT1004430.1219086212697246No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAAT964680.1170841260879085No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT954640.11586556177028752No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA946770.11491037240976192No Hit
TCACTCGCCGAATCCCGGGGCCGAGGGAGCCTGTAGGCACCATCAAT931290.11303155013518296No Hit
ACGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT902920.10958825634126793No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA870790.10568860778298486No Hit
TGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAAT867480.10528686994520345No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT865860.1050902490094917No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGACTGTAGGCACCATCAAT856890.10400155160619885No Hit
TGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATC853430.10358160812622191No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT848160.10294198323041887No Hit
TTCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAAT838650.1017877455152221No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACTGTAGGCACCATCAAT831690.10094300371734938No Hit
TCTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAAT824070.10001815709381634No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)