FastQCFastQC Report
Wed 23 Aug 2023
SRR8590814_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8590814_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93149050
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT10932031.1736061720436226No Hit
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC9482831.0180275590572314No Hit
CGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATA8838870.9488953456852217No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG8062420.8655396914944382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7775800.834769651434985No Hit
GTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCAT6259340.6719703528914144No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC5150730.5529557198919366No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG4323820.4641829412108873No Hit
CCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATA4219410.45297402388966934No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG4015790.4311144343393733No Hit
CCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGG3746040.40215547018461273No Hit
CTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCC3539660.3799995813161809No Hit
CTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGC3120000.33494705528397767No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC2970420.3188889204989208No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA2940760.3157047763772148No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC2815970.30230796771410984No Hit
GTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCA2815540.3022618051391829No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG2772420.2976326650674376No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG2730080.2930872617595134No Hit
GTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGCATCGG2648870.2843689763878429No Hit
GTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCC2611560.28036356785173866No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2598130.2789217925464618No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC2480760.2663215566879104No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG2475230.26572788450338464No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT2468840.26504188716900495No Hit
TGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCA2463360.26445358272574976No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT2428800.2607433999595272No Hit
GGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCAT2387430.2563021308322522No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC2219420.23826544661486082No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC2201760.23636956039809315No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT2148550.23065721013794557No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG2146000.23038345533314616No Hit
GCCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCG2133510.22904259356375614No Hit
CCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG2096550.22507475921654593No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC2020750.21693726345035189No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC2010240.2158089642352767No Hit
GGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCGATGCTTT1992620.2139173722115255No Hit
CCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGC1950700.2094170579302741No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT1946050.2089178579921105No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA1927640.20694145565628422No Hit
CTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGGA1923190.2064637266832029No Hit
CTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTC1894230.20335473093928497No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1865970.20032088357315506No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1840520.1975887032664316No Hit
GCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGA1840470.197583335525161No Hit
GCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGCAT1834280.19691880915586363No Hit
GGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATC1819600.19534284031882237No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT1811920.1945183552596618No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA1735250.1862874607953597No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT1702830.18280701735551785No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT1695150.1819825322963573No Hit
CGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAA1687810.18119454787783665No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC1676780.18001042415354745No Hit
GCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATT1674080.17972056612493634No Hit
CTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCC1667610.17902598040452372No Hit
GTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGG1630280.17501842477191124No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC1630240.17501413057889478No Hit
CGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGA1617340.17362925333108606No Hit
CACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGA1614960.17337374884660658No Hit
GCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCG1609690.17280798891668783No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG1600450.17181603032988527No Hit
GGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGA1597770.17152831939778238No Hit
GATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTAT1578040.16941020869241286No Hit
CCGCGCTTCATTGAATTTCTTCACTTTGACATTCAGAGCACTGGGCAGAA1573830.1689582448774303No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCG1566330.16815308368684384No Hit
GCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATC1554540.16688737029524187No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT1516410.16279393080230017No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC1508360.16192972445773735No Hit
CCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCT1481090.1590021583687649No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA1471830.15800805268545412No Hit
GGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATC1454660.1561647703331381No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG1444360.15505901563139934No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT1439790.15456840407926867No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC1435860.15414649961540133No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT1424300.15290547783364403No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT1418580.1522914082322901No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA1418510.1522838933945113No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT1409480.15131447932104514No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC1373600.14746258818527938No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1368550.14692044631695117No Hit
CTAGGCTTCAAGGCTCACCGCAGCGGCCCTCCTACTCGTCGCGGCGTAGC1347820.14469498078617013No Hit
GGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAA1347100.14461768531187383No Hit
GTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA1337460.14358278479490666No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC1330960.1428849784297317No Hit
CTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCC1325190.14226554108710715No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC1312030.1408527515846914No Hit
GTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTT1299040.13945821240259562No Hit
CTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATTGAATT1292600.13876684732694536No Hit
ACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATT1282830.1377179906826747No Hit
GCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGTGTCCTGCAATTCACA1281370.13756125263757385No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCC1262170.13550003998967247No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTT1250770.134276194979981No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC1249210.13410872145233904No Hit
CCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACC1227380.1317651656136053No Hit
GTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAAT1219980.13097073990555996No Hit
GTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCC1217670.13072275025885932No Hit
GTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCATCTCCCTGCTCC1209210.12981452843587776No Hit
GCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGC1203520.12920367947928615No Hit
GTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCC1194400.128224603471533No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA1192760.12804854155785808No Hit
CTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTAC1178420.12650907336145673No Hit
CCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCG1169180.1255171147746542No Hit
GTCCCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAA1160950.12463358456151725No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGAT1158440.12436412394973431No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT1150570.12351924147374557No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC1150230.12348274083310565No Hit
GCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC1148990.12334962084959535No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATC1145660.12299212928097494No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCT1135330.12188315393447384No Hit
CCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGG1133880.12172748943762711No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGC1131920.1215170739798205No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTA1122560.12051223281396858No Hit
GTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGC1119300.120162256083127No Hit
CGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCG1116620.11987454515102408No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1102450.11835332727494269No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100510.11814505891364431No Hit
TGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCG1087310.11672797521821211No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG1085190.11650038298833966No Hit
GTTCGAAGTGTCGATGATCAATGTGTCCTGCAATTCACATTAATTCTCGC1082430.11620408367020382No Hit
GCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGT1073360.11523037540372123No Hit
GTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGA1059880.1137832323571738No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG1056250.11339353434092994No Hit
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTC1052420.11298236535960378No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG1050390.11276443506401837No Hit
GTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGG1045130.11219974868235372No Hit
CTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCA1042040.11186802227183207No Hit
GTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCAC1038370.11147403006257176No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGG1035640.11118095138919828No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC1032350.11082775401359433No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA1030860.11066779532373117No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG1020050.10950728966103251No Hit
GTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACCAGAGT1018380.10932800710259526No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT1018290.10931834516830821No Hit
GCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTAT1014040.1088620871603092No Hit
GTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGT1000180.10737414928010539No Hit
CCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGC972990.10445517157716584No Hit
GTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCC960210.10308317690840647No Hit
CCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGG955620.10259041825976754No Hit
CTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTT943390.10127746874498453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)