FastQCFastQC Report
Fri 14 Jul 2023
SRR8594298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8594298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138279487
Total Bases6.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG24601031.7790802188903116TruSeq Adapter, Index 2 (100% over 49bp)
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGGAGATCGGAAGAGCACACG15108261.0925886642897367No Hit
GTTGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACAC1672670.12096298853061264No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGT1644960.11895907597632323TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)