FastQCFastQC Report
Fri 14 Jul 2023
SRR8594299_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8594299_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135806383
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG86860946.395939430917617TruSeq Adapter, Index 1 (100% over 49bp)
CGGCCGAGGGCACGCCTGCCTGGGCGTCACGCAGATCGGAAGAGCACACG57918724.264801014544361No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCCGAGATCGGAAGAGCA20940921.5419687600397989No Hit
AGCCGTCGTCGGCGCAGCCGGTCACCGCGCGAGATCGGAAGAGCACACGT19924631.467135016768689No Hit
TCATGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACG15640721.1516925533610596No Hit
CGCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCAGATCGGAAGAGC13190040.9712385904571216No Hit
GCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCAGATCGGAAGAGCA11871020.8741135532635457No Hit
TCATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT11568560.8518421405862786No Hit
AGAACGTGAAACCGTGCTGAGCTCCCAAGCAGATCGGAAGAGCACACGTC9943920.7322130065123669No Hit
AGTCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGT8567160.6308363282158836No Hit
TCCGATGATTCATGATAACTCGACGGATCGCACAGATCGGAAGAGCACAC8533780.6283784172353666No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC6126560.45112459846603825No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGT6099860.4491585642185905TruSeq Adapter, Index 1 (100% over 50bp)
GACTGTTGTCGGCCGCGCCGGCGGCCAAAGCCAGATCGGAAGAGCACACG4989520.36739952053652736No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGGAGATCGGAAGAGCACACG4813470.3544362123244237No Hit
GCGCTCTTAGTTCAGTTCGGTAGAACGCGGGAGATCGGAAGAGCACACGT4777830.35181188795816765No Hit
ACGCGCGTGCCCCGAAAGGGAATCGGGTTAGATCGGAAGAGCACACGTCT4275850.3148489714213212Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCCGTAGATCGGAAGAGC4183160.30802381357877706No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCCAGATCGGAAGAGCAC4033260.29698604078130847No Hit
ATAAACGATGCCGACCAGGGATCGGCGGATGTAGATCGGAAGAGCACACG3902200.2873355370932749No Hit
GGGAGACCGGGCTTGGACCATGTCTCCCGAACAATAGATCGGAAGAGCAC3863530.2844881009753422No Hit
CGCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCCAGATCGGAAGAG3787710.27890515278652256No Hit
GCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCCAGATCGGAAGAGC3471140.25559476096200867No Hit
TCCGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG3419390.25178418896555105No Hit
GGGAGAGTGACACCCAGGCAGACGTGCCCTCAGATCGGAAGAGCACACGT3393850.24990357043821718No Hit
AGCGCGCGACCCACACCAGGCCATCTGGGCGAGATCGGAAGAGCACACGT3336630.24569021914087794No Hit
GTTGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACAC3325550.2448743517453079No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATGAGATCGGAAGAGCACACGTCTG3283990.24181411266950537Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCGCGACCCACACCAGGCCATCTGGGCAGATCGGAAGAGCACACGTC3176260.23388149583514053No Hit
GCGCCCCCGGCAGCCGTCGTCGGCGCAGCCGGTCACAGATCGGAAGAGCA3044450.2241757664659989No Hit
GGGAGACCGGGCTTGGACCATGTCTCCCGAACAATCAGATCGGAAGAGCA3043190.22408298732173731No Hit
TTGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACACG3042810.22405500630997588No Hit
CCGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC2835710.2088053548999976No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG2653070.19535679703655753No Hit
TCCGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT2644020.19469040715118668No Hit
AGGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTTGAGATCGGAAGAGC2264100.16671528612907685No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTAGATCGGAAGAGCACACGTCT2263910.16670129562319616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCCCCGGCAGCCGTCGTCGGCGCAGCCGGTCAGATCGGAAGAGCACA2253860.16596127149634787No Hit
AAGTCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACG2253140.1659082548424841No Hit
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTTGAGATCGGAAGAGCA2242210.16510343258313565No Hit
GCGCTCTTAGTTCAGTTCGGTAGAACGCGGGTAGATCGGAAGAGCACACG2182220.1606861144369039No Hit
GCGCCCCCGGCAGCCGTCGTCGGCGCAGCCGGTCACCAGATCGGAAGAGC2148030.15816855972079014No Hit
GCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGAGATCGGAAGAGCAC2142360.15775105357161306No Hit
CGCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGAGATCGGAAGAGCA2111070.15544703815578387No Hit
CCGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT2058340.15156430460267836No Hit
CGCCCGCCGCCCGCCCCGACCCACGTTAGGGGCGAGATCGGAAGAGCACA2048250.15082133510617096No Hit
TCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGTCT2015370.14840024124639267Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCCGAGGGCACGTCTGCCTGGGTGTCACAAGATCGGAAGAGCACACGT1994640.14687380342056527No Hit
TGTTGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACA1973240.14529803065294805No Hit
GCGCCCCCGGCAGCCGTCGTCGGCGCAGCCGGTCACCGAGATCGGAAGAG1880830.1384934903980176No Hit
AGAACGTGAAACCGTGCTGAGCTCCCAAGAGATCGGAAGAGCACACGTCT1831290.13484565007522512Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGCGTGCCCCGAAAGGGAATCGGGTTAAGATAGATCGGAAGAGCACAC1807740.13311156368843136No Hit
GGACTGTTGTCGGCCGCGCCGGCGGCCAAAGCCAGATCGGAAGAGCACAC1799430.1324996631417538No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCAGATCGGAAGAGCACA1788390.13168674111584286No Hit
AACTTTAAATAGGTAGGACGGCGCGGCTAGATCGGAAGAGCACACGTCTG1773920.13062125364166427Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACGT1742900.128337119471034No Hit
GGCCCCTCCTTTTGGCTCTAAGGCCCGAGTAGATCGGAAGAGCACACGTC1726390.1271214181442414No Hit
GGGAGAGGTGATCCAGCCGCACCTTCCAGTACGAGATCGGAAGAGCACAC1686420.12417825751238806No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACAC1639040.12068946715118685No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG1611170.11863728084121053TruSeq Adapter, Index 1 (100% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC1569680.11558219616231145TruSeq Adapter, Index 1 (100% over 50bp)
CGCCCCCGGCAGCCGTCGTCGGCGCAGCCGGTCACCGAGATCGGAAGAGC1566420.11534214853509499No Hit
CAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGTCTG1539600.11336727817866998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAACGATGCCGACCAGGGATCGGCGGATGAGATCGGAAGAGCACACGT1523870.11220901156022985No Hit
GCGCCCCCGGCAGCCGTCGTCGGCGCAGCCGGTCAAGATCGGAAGAGCAC1491880.10985345217536646No Hit
GCCGAGGGCACGCCTGCCTGGGCGTCACGCAGATCGGAAGAGCACACGTC1489050.10964506727198531No Hit
GGGAGAGTGACACCCAGGCAGACGTGCCCTCGGAGATCGGAAGAGCACAC1426150.10501347348305418No Hit
GTTGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGGAGATCGGAAGAGCACA1400470.10312254616191346No Hit
CTGCCACGATCCACTGAGATCCAGCCCCGCAGATCGGAAGAGCACACGTC1399810.10307394756253836No Hit
TGGCCCCTCCTTTTGGCTCTAAGGCCCGAGAGATCGGAAGAGCACACGTC1378810.10152762849151206No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)