FastQCFastQC Report
Fri 14 Jul 2023
SRR8594308_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8594308_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181383428
Total Bases13.1 Gbp
Sequences flagged as poor quality0
Sequence length50-75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG34925631.9255138347037966TruSeq Adapter, Index 11 (100% over 49bp)
CGAGGGCACGCCTGCCTGGGCGTCACGCAGATCGGAAGAGCACACGTCTG34676161.911760097510121Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGTGTCCGGTGCGCCCCCGGCGGCCAGATCGGAAGAGCACACGTCTG19862781.0950713755393355Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCTG16560880.9130315918386988Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCGTCGTCGGCGCAGCCGGTCACCGCGCGAGATCGGAAGAGCACACGT15700630.8656044365861251No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC13521150.7454457195505203No Hit
AATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGTCTGAAC12931770.7129521226161852Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGTCCACGCCCGGTCGTACTCATAACCAGATCGGAAGAGCACACGTCT12901710.7112948598589723Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGA12071360.6655161462710916Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAATAGATCGGAAGAGCACAC10969740.6047818216336721No Hit
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAAAGATCGGAAGAGCACACG10794270.5951078397305404No Hit
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGTC10552070.5817549109282464No Hit
ACTATGCCTGAGCGGGGCGAAGCCAGAGAGATCGGAAGAGCACACGTCTG9354310.5157202123228148Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATTTCGTATG8354140.4605790116614181TruSeq Adapter, Index 11 (97% over 49bp)
TCCCAGTAAAACGCGGCGTGTTCGAATGAGATCGGAAGAGCACACGTCTG8253150.45501124832639067Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCCTCCTTTTGGCTCTAAGGCCCGAAGATCGGAAGAGCACACGTCTGA7972540.43954070600099143Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTTAAATAGGTAGGACGGCGCGGCTAGATCGGAAGAGCACACGTCTGA7947220.4381447681096864Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCCGAGGGCACGCCTGCCTGGGCGTCACGCAGATCGGAAGAGCACACG7942880.4379054959750789No Hit
TGCTTAACGGCCCGCCAACCCTGGAAAGATCGGAAGAGCACACGTCTGAA7867500.4337496587615491Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCACCACCAGGCGTGGAGCCTGCGGCTTAATAGATCGGAAGAGCACA7717190.42546279365720224No Hit
TGTCCTAAGATGAGCTCAACGAGAACAGATCGGAAGAGCACACGTCTGAA7507810.4139192914580928Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGTCTGAA7415780.4088455093041907Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGTCTGA7360130.40577742306204506Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCAGATCGGAAGAGCACA6695330.36912578364105014No Hit
ATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCTGA6443380.355235319513313Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAAGATCGGAAGAGCACACGT5827520.3212818317669021No Hit
GGGCCGATCCGGGCGGAAGACATTGTCAGGAGATCGGAAGAGCACACGTC5553840.3061933530112795No Hit
AGTCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGT5184620.28583757938459514No Hit
CCGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC5100840.28121863481376036No Hit
AGCGCGCGACCCACACCAGGCCATCTGGGCAGATCGGAAGAGCACACGTC4958410.2733662085160282No Hit
ATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGAA4864350.26818050875077737Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGAAGAAACTTACGAGGATTCCCCAGATCGGAAGAGCACACGTCTGAA4676510.25782454613218575Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCACCACCAGGCGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGT4564410.2516442681852942No Hit
TTGATCCTTCGATGTCGGCTCTTCCTATAGATCGGAAGAGCACACGTCTG4405860.2429031168161625Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTTAAATAGGTAGGACGGCGCGGCAGATCGGAAGAGCACACGTCTGAA4298630.2369913308728513Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGTTGTCGGCCGCGCCGGCGGCCAAAGCAGATCGGAAGAGCACACGT4083420.22512641011504095No Hit
GACTGTTGTCGGCCGCGCCGGCGGCCAAAGCCAGATCGGAAGAGCACACG3967360.218727810128277No Hit
AGGGCACCACCAGGCGTGGAGCCTGCGGCTTAAAGATCGGAAGAGCACAC3951260.21784018769344243No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCCAGATCGGAAGAGCAC3842140.2118242025947376No Hit
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTAGATCGGAAGAGCACA3718130.2049873045733814No Hit
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTTGAGATCGGAAGAGCA3655800.20155093771852187No Hit
AGTCAGAATCCAAGCTAGCAAGCGGCGCCTAGATCGGAAGAGCACACGTC3615760.19934345931536812No Hit
ACTATGCCTGAGCGGGGCGAAGCCAGAAGATCGGAAGAGCACACGTCTGA3539690.19514958114034542Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTTAAATAGGTAGGACGGCGCGGCTAGATCGGAAGAGCACACGTCTG3531200.19468151191849786Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGTCTG3140380.17313489080160066Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGTC2969650.16372223376437675No Hit
CGGGCCGATCCGGGCGGAAGACATTGTCAGGAGATCGGAAGAGCACACGT2920020.16098604112829978No Hit
AAGCCAGTGGCGCGAAGCTACCGTGTGCCGGAAGATCGGAAGAGCACACG2875690.15854204718195095No Hit
CGTAGGTTCGAATCCTACTTGGGGAAGATCGGAAGAGCACACGTCTGAAC2832310.15615042847244015Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCCCCCGGCAGCCGTCGTCGGCGCAGCCGGTCAGATCGGAAGAGCACA2815320.15521373871046257No Hit
GGGCACCACCAGGCGTGGAGCCTGCGGCTAGATCGGAAGAGCACACGTCT2774650.1529715272555109Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTATGCCTGAGCGGGGCGAAGCCAGAGAGATCGGAAGAGCACACGTCT2759120.15211532996277918Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGTGTCCGGTGCGCCCCCGGCGGCCCAGATCGGAAGAGCACACGTCT2749670.15159433418581106Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCTTAACGGCCCGCCAACCCTGGAAAGATCGGAAGAGCACACGTCTGA2728850.1504464895216337Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCTCCTTTTGGCTCTAAGGCCCGAAGATCGGAAGAGCACACGTCTGAA2614020.14411570168361798Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGGCACGCCTGCCTGGGCGTCACGCAGATCGGAAGAGCACACGTCTGA2513800.13859038985634342Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCAGATCGGAAGAGCA2485810.13704724998360931No Hit
CCGATGATTCATGATAACTCGACGGATCGAGATCGGAAGAGCACACGTCT2414870.13313619808751218Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCAGTGGCGCGAAGCTACCGTGTGCCGGAAGATCGGAAGAGCACACGT2384530.13146349841838914No Hit
GAACCCAGAGCTAGCTGGTTCTCCCCGAAATAGATCGGAAGAGCACACGT2383500.13140671263529102No Hit
CCGGAGGTAGGGTCCAGCGGCCGGAAGAGCAGATCGGAAGAGCACACGTC2376240.1310064555621917No Hit
AGGGCACCACCAGGCGTGGAGCCTGCGGCTAGATCGGAAGAGCACACGTC2355720.12987515044648953No Hit
CCGGAGGTAGGGTCCAGCGGCCGGAAGAGCAAGATCGGAAGAGCACACGT2272170.1252688861961524No Hit
GCCCCTCCTTTTGGCTCTAAGGCCCGAGAGATCGGAAGAGCACACGTCTG2247310.12389830894584261Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTCTACTATCCAGCGAAACCACAGCCAGATCGGAAGAGCACACGT2201120.12135176979894767No Hit
GGGCCGATCCGGGCGGAAGACATTGTCAGAGATCGGAAGAGCACACGTCT2197400.12114667939785546Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCGCGACCCACACCAGGCCATCTGGGCGAGATCGGAAGAGCACACGT2166310.1194326308575445No Hit
GCCCCTCCTTTTGGCTCTAAGGCCCGAGATCGGAAGAGCACACGTCTGAA2137720.11785641188786002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCCACGATCCACTGAGATCCAGCCCCGCAGATCGGAAGAGCACACGTCT2131540.11751569718927134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTAAATAGGTAGGACGGCGCGGCAGATCGGAAGAGCACACGTCTGA2082190.11479494146510452Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTAGATCGGAAGAGCAC2037690.11234157510795308No Hit
CCCCTCCTTTTGGCTCTAAGGCCCGAGAGATCGGAAGAGCACACGTCTGA1985900.10948629772285481Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCAGTAAAACGCGGCGTGTTCGAATAGATCGGAAGAGCACACGTCTGA1979050.10910864469933824Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAATCCAAGCTAGCAAGCGGCGCCTAGATCGGAAGAGCACACGTCTGAA1961910.10816368516312305Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTCCTAAGATGAGCTCAACGAGAACAAGATCGGAAGAGCACACGTCTGA1960730.10809862960578737Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCATTAATGCGGTACGTGAGCTGGAGATCGGAAGAGCACACGTCTGAA1946910.10733670773936414Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAGGGCACGCCTGCCTGGGCGTCACGAGATCGGAAGAGCACACGTCTGA1939110.1069066794790095Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG1933550.10660014651393622No Hit
AGGGCACCACCAGGCGTGGAGCCTGCGGCTTAAGATCGGAAGAGCACACG1888170.10409826414792425No Hit
GCCACGATCCACTGAGATCCAGCCCCGCAGATCGGAAGAGCACACGTCTG1874030.10331870009646085Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGCCACGATCCACTGAGATCCAGCCCCGCAGATCGGAAGAGCACACGTC1868300.10300279472058496No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)