FastQCFastQC Report
Mon 24 Jul 2023
SRR8594309_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8594309_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164697539
Total Bases11.9 Gbp
Sequences flagged as poor quality0
Sequence length50-75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG71678714.35214214099459TruSeq Adapter, Index 9 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATTTCGTATG43940382.667943933272737TruSeq Adapter, Index 9 (97% over 49bp)
TGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACACGT34794032.1126016946737742No Hit
TCGGTTCCTATCTACCGTTGGTGTTAAAGATCGGAAGAGCACACGTCTGA28695161.7422944006467516Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGTGTCCGGTGCGCCCCCGGCGGCCAGATCGGAAGAGCACACGTCTG22906471.390820417784142Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCGGTTCCTATCTACCGTTGGTGTTAAAGATCGGAAGAGCACACGTCTG14826040.9001980290670888Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCTTAACGGCCCGCCAACCCTGGAAAGATCGGAAGAGCACACGTCTGAA13553870.8229552233928645Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCCGCAGCTAGCTTCTTAGAGGGAAGATCGGAAGAGCACACGTCTGAA10042560.6097577450747458Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGAA9325590.5662252184593967Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGTCCACGCCCGGTCGTACTCATAACCAGATCGGAAGAGCACACGTCT6935710.4211180107554613Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATGTCGTATG6191130.375909077791381TruSeq Adapter, Index 9 (97% over 49bp)
CTGGGCCGCACGCGCGCTACACTGATGTAGATCGGAAGAGCACACGTCTG5995870.3640534058010424Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG5897820.3581000685140778Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCAGTAAAACGCGGCGTGTTCGAAAGATCGGAAGAGCACACGTCTGAA5803160.3523525630823178Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTCCTAAGATGAGCTCAACGAGAACAGATCGGAAGAGCACACGTCTGAA5655220.34337003663424503Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACGGAGTCAAGACCCGCAACGCGAAGATCGGAAGAGCACACGTCTGAA5456010.33127453106630816Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTATGCCTGAGCGGGGCGAAGCCAGAGAGATCGGAAGAGCACACGTCTG5245200.3184747041059308Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGGGCACGCCTGCCTGGGCGTCACGCAGATCGGAAGAGCACACGTCTG5177770.31438053242556346Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGA5107400.3101078517026293Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGATCCTTCGATGTCGGCTCTTCCTATAGATCGGAAGAGCACACGTCTG4964480.30143012640887124Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTATGCCTGAGCGGGGCGAAGCCAGAAGATCGGAAGAGCACACGTCTGA4760620.289052285110344Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAA4722910.28676263341129826Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAATCCAAGCTAGCAAGCGGCGCCTAGATCGGAAGAGCACACGTCTGAA4543900.2758936185439905Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG4323130.262489046663897TruSeq Adapter, Index 9 (100% over 50bp)
GGAGAAGAAACTTACGAGGATTCCCCAGATCGGAAGAGCACACGTCTGAA4227490.2566820382179481Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACGGAGTCAAGACCCGCAACGCAGAGATCGGAAGAGCACACGTCTGAA3900300.23681592473582738Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCCCCGGCAGCCGTCGTCGGCGCAGCCGGAGATCGGAAGAGCACACG3755180.22800462124695137No Hit
CCTCCGCAGCTAGCTTCTTAGAGGGACAGATCGGAAGAGCACACGTCTGA3608230.21908220498668166Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGCTTAACGGCCCGCCAACCCTGGAAAGATCGGAAGAGCACACGTCTGA3457320.20991934797519957Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG3395140.2061439424422729Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCAGTAAAACGCGGCGTGTTCGAATGAGATCGGAAGAGCACACGTCTGA3357350.2038494333543138Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACACG3272510.19869817241167154No Hit
GAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG3252980.19751236234319203Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACGGAGTCAAGACCCGCAACGCGCAGATCGGAAGAGCACACGTCTGAA3093410.18782369298183624Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGTTCCTATCTACCGTTGGTGTTAAAGATCGGAAGAGCACACGTCTGAA3039140.1845285617777203Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG3029330.18393292446221676Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACATGACGAAGTGAGCGGGCTCTAGATCGGAAGAGCACACGTCTGAA2966410.18011258808184133Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGGCGTGGAGCCTGCGGCTTAATTTGAAGATCGGAAGAGCACACGTCTG2938270.17840400153155903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC2831480.1719199945058074No Hit
CGGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC2829350.1717906665259886No Hit
AGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG2816150.17098919735528045Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCGCAGCTAGCTTCTTAGAGGGACTAGATCGGAAGAGCACACGTCTG2684970.16302429388456133Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC2584540.15692644927742364TruSeq Adapter, Index 9 (100% over 50bp)
TCGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG2560790.15548441194376317Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGTTCCCTCCGAAGTTTCCCTCAGGAGATCGGAAGAGCACACGTCTGA2439080.1480945018856657Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGCCGCACGCGCGCTACACTGATGTAGATCGGAAGAGCACACGTCTGA2435490.14787652655817765Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG2396930.14553526510192724Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGGAGATCGGAAGAGCACACG2384790.14479815633432142No Hit
CAGAATCCAAGCTAGCAAGCGGCGCCTAGATCGGAAGAGCACACGTCTGA2364480.14356498672393642Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTTAAATAGGTAGGACGGCGCGGCTAGATCGGAAGAGCACACGTCTGA2309390.14022006728345832Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACACGTC2300960.13970821992671062No Hit
CGCGGTGTCCGGTGCGCCCCCGGCGGCCCAGATCGGAAGAGCACACGTCT2284220.1386918112965853Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCTG2254540.13688972000972036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTACCCTACTGATGACCGTGCCGCGAGATCGGAAGAGCACACGTCTGAA2190450.13299834431648672Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG2162760.13131708057884217Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACGGAGTCAAGACCCGCAACGCAAAGATCGGAAGAGCACACGTCTGAA2155330.13086595058351175Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC2134770.12961760163277244No Hit
GGGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG2049770.12445662591230339Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGT2041560.12395813637506752TruSeq Adapter, Index 9 (100% over 50bp)
GGTGCGTGCCGCACTCACGAGGGACTGCCAGTAGATCGGAAGAGCACACG2024740.12293687035602882No Hit
TTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG2018040.12253006403453302Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGAGCCCATTACGAGTTCTATCGGGAGATCGGAAGAGCACACGTCTGA1965920.11936547515746425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGTACACGGAGTCGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTG1951060.11846321516680344Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTGCGTGCCGCACTCACGAGGGACTGCCAAGATCGGAAGAGCACACGT1902080.11548927880458493No Hit
GAGCTTGACTCTAGTCCGACTTTGTGAAGATCGGAAGAGCACACGTCTGA1887920.11462952096691621Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTCTCTGGGTCCCTACCGACGCTGAGATCGGAAGAGCACACGTCTGAA1827460.1109585492956273Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1811030.10996096304754135Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGCGCTACACTGATGTATTCAACGAGATCGGAAGAGCACACGTCTGAA1802210.10942543591984091Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATTAAGCCATGCATGTGCAAGTATGAGATCGGAAGAGCACACGTCTGA1728900.10497424615433992Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACACGGAGTCAAGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTGA1665900.10114905238505112Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGTACACGGAGTCGACCCGCAACGCGTAGATCGGAAGAGCACACGTCTG1661540.10088432468927178Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGTACACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTG1661180.10086246643916155Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)