FastQCFastQC Report
Mon 17 Jul 2023
SRR8594310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8594310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135127676
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG42847633.170899646050303TruSeq Adapter, Index 4 (100% over 49bp)
CGGCCGAGGGCACGCCTGCCTGGGCGTCACGCAGATCGGAAGAGCACACG32678622.4183513671914256No Hit
TCATGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACG24555311.817193244705844No Hit
AGCCGTCGTCGGCGCAGCCGGTCACCGCGCGAGATCGGAAGAGCACACGT16643531.231689206288133No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGGAGATCGGAAGAGCACACG16349111.209900923627222No Hit
TCATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT16203511.1991259288733715No Hit
CGCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCAGATCGGAAGAGC14762061.0924527407694038No Hit
AGTCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGT12498280.9249237735724841No Hit
GCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCAGATCGGAAGAGCA10814550.8003208757915736No Hit
TCCGATGATTCATGATAACTCGACGGATCGCACAGATCGGAAGAGCACAC9933550.7351232770405968No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCCGAGATCGGAAGAGCA9249470.6844985626778632No Hit
AGCGCGCGACCCACACCAGGCCATCTGGGCAGATCGGAAGAGCACACGTC8118110.6007733012443728No Hit
AGCGCGCGACCCACACCAGGCCATCTGGGCGAGATCGGAAGAGCACACGT7971590.5899302227324623No Hit
CGCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCCAGATCGGAAGAG6420970.47517800868565224No Hit
GCGCTCTTAGTTCAGTTCGGTAGAACGCGGGAGATCGGAAGAGCACACGT5146830.3808864440175823No Hit
GGCCCCTCCTTTTGGCTCTAAGGCCCGAGTAGATCGGAAGAGCACACGTC4501090.33309904626791625No Hit
GGGAGACCGGGCTTGGACCATGTCTCCCGAACAATAGATCGGAAGAGCAC4406550.3261027000863983No Hit
GCCCCTCCTTTTGGCTCTAAGGCCCGAGTAGATCGGAAGAGCACACGTCT4062730.3006586156340023Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGCGTGCCCCGAAAGGGAATCGGGTTAAGATAGATCGGAAGAGCACAC4051870.2998549312725544No Hit
TCCGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT3935720.2912593568174739No Hit
GCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCCAGATCGGAAGAGC3828550.2833283390443272No Hit
GGGAGACCGGGCTTGGACCATGTCTCCCGAACAATCAGATCGGAAGAGCA3814520.2822900617339116No Hit
GACTGTTGTCGGCCGCGCCGGCGGCCAAAGCCAGATCGGAAGAGCACACG3756940.278028906528371No Hit
AGAACGTGAAACCGTGCTGAGCTCCCAAGCAGATCGGAAGAGCACACGTC3754440.27784389631625134No Hit
AAGTCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACG3656080.27056485453061446No Hit
GGACTGTTGTCGGCCGCGCCGGCGGCCAAAGCCAGATCGGAAGAGCACAC3425050.2534676908082102No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCCGTAGATCGGAAGAGC3313920.24524361685906593No Hit
GCGCTCTTAGTTCAGTTCGGTAGAACGCGGGTAGATCGGAAGAGCACACG3259090.24118597288685703No Hit
TGGCCCCTCCTTTTGGCTCTAAGGCCCGAGAGATCGGAAGAGCACACGTC3128470.23151955932402776No Hit
TCCGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG3048400.22559405225025847No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCCAGATCGGAAGAGCAC2733310.20227610515554192No Hit
TGGCCGAGGGCACGTCTGCCTGGGTGTCACAAGATCGGAAGAGCACACGT2587610.19149370999320672No Hit
AGGGCTATAGCTCAGTTCGGTAGAGCAACTCGTAGATCGGAAGAGCACAC2471450.1828973954972777No Hit
CGCCCGCCGCCCGCCCCGACCCACGTTAGGGGCGAGATCGGAAGAGCACA2432630.18002455692348324No Hit
CCGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC2410310.17837278574967871No Hit
CCGTTGTAGTCTAGCTGGTTAGGATACTCGGAGATCGGAAGAGCACACGT2379180.1760690385883644No Hit
GGGAGAGTGACACCCAGGCAGACGTGCCCTCAGATCGGAAGAGCACACGT2318120.1715503491675532No Hit
TCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGTCT2280530.16876853561812163Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGAGATCGGAAGAGCA2130050.15763240093021358No Hit
ATCATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACG2074860.15354811548745942No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACAGATCGGAAGAGCACACG2043810.15125028865293294No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCAGATCGGAAGAGCACAC1960540.14508796850765052No Hit
CTGCCACGATCCACTGAGATCCAGCCCCGCAGATCGGAAGAGCACACGTC1956730.1448060129443801No Hit
GCTTTCGTAGCTCAGTTGGTTAGAGCACCCGTTAGATCGGAAGAGCACAC1892130.14002534906320743No Hit
ACGCGCGTGCCCCGAAAGGGAATCGGGTTAGATCGGAAGAGCACACGTCT1890360.13989436183302673Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTTGAGATCGGAAGAGC1890350.13989362179217824No Hit
CATGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACGT1845420.13656861825996328No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATGAGATCGGAAGAGCACACGTCTG1841780.136299243391117Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT1820890.13475329805864494TruSeq Adapter, Index 4 (100% over 50bp)
CCCTCCGCAGCTAGCTTCTTAGAGGGACTAGATCGGAAGAGCACACGTCT1803670.13347894771756455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGCGTGCCCCGAAAGGGAATCGGGTTAAGATAGATCGGAAGAGCACA1765010.13061794979734573No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC1756650.12999927564801753No Hit
GGGAGACCGGGCTTGGACCATGTCTCCCGAACAGATCGGAAGAGCACACG1708390.12642783851325912No Hit
AGATCCAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGAGCACACGTCT1677720.12415813323097484Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACACG1663280.12308951424577154No Hit
GCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGAGATCGGAAGAGCAC1627550.12044534829415701No Hit
GGGTTGCTAACTCAATGGTAGAGTACTCGGAGATCGGAAGAGCACACGTC1605960.11884760010229141No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCAGATCGGAAGAGCACA1581360.11702709961503371No Hit
GTTGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACAC1579710.11690499287503471No Hit
AGGTGCGTGCCGCACTCACGAGGGACTGCCAGAGATCGGAAGAGCACACG1554130.11501196838462611No Hit
TGTTGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACA1479320.10947572279715667No Hit
AAGCGCGCGACCCACACCAGGCCATCTGGGCGAGATCGGAAGAGCACACG1460680.10809628665559229No Hit
AGCCGTCGTCGGCGCAGCCGGTCACCGCGCGCCAGATCGGAAGAGCACAC1438220.10643415490990905No Hit
CCGCCGCGTGCCGGCCGGGGGACGGACCGGGAAGATCGGAAGAGCACACG1423940.10537737657828142No Hit
AAGCGCGCGACCCACACCAGGCCATCTGGGCAGATCGGAAGAGCACACGT1416190.10480384492071039No Hit
GGGAGAGGTGATCCAGCCGCACCTTCCAGTACGAGATCGGAAGAGCACAC1404980.10397425912956573No Hit
TCATGCATTGGATGGATGCCCGGGCATTGAAGATCGGAAGAGCACACGTC1387670.10269324842084902No Hit
GGGAGACCGGGCTTGGACCATGTCTCCCGAACAAAGATCGGAAGAGCACA1386390.10259852319224375No Hit
ATGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACGTC1367680.10121390676474003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)