FastQCFastQC Report
Fri 14 Jul 2023
SRR8594311_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8594311_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137037870
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG34442222.513335912182523TruSeq Adapter, Index 3 (100% over 49bp)
CGGCCGAGGGCACGCCTGCCTGGGCGTCACGCAGATCGGAAGAGCACACG33623092.453561924160088No Hit
AGAACGTGAAACCGTGCTGAGCTCCCAAGCAGATCGGAAGAGCACACGTC21075151.537907003370674No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCCGAGATCGGAAGAGCA18658291.361542615920694No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGGAGATCGGAAGAGCACACG9783910.7139566603012729No Hit
AGCCGTCGTCGGCGCAGCCGGTCACCGCGCGAGATCGGAAGAGCACACGT9002850.6569607364737937No Hit
CGCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCAGATCGGAAGAGC8762460.6394188701269219No Hit
GCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCAGATCGGAAGAGCA7838710.5720104960767415No Hit
TCATGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACG7153000.5219725029292998No Hit
TCCGATGATTCATGATAACTCGACGGATCGCACAGATCGGAAGAGCACAC6836750.49889494050075356No Hit
AGTCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGT6035280.44040964734784627No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCCGTAGATCGGAAGAGC5970990.435718243431542No Hit
TCATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT5956810.4346834929643901No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCCAGATCGGAAGAGCAC5115220.37327054193121945No Hit
ACGCGCGTGCCCCGAAAGGGAATCGGGTTAGATCGGAAGAGCACACGTCT4103420.29943693666575527Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCCAGATCGGAAGAG3964050.2892667552407229No Hit
AGAACGTGAAACCGTGCTGAGCTCCCAAGAGATCGGAAGAGCACACGTCT3718760.2713673234997012Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGACCGGGCTTGGACCATGTCTCCCGAACAATAGATCGGAAGAGCAC3051200.2226537817612022No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCAGATCGGAAGAGCACA2975510.21713049100952897No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGT2846720.2077323589457425TruSeq Adapter, Index 3 (100% over 50bp)
AGCGCGCGACCCACACCAGGCCATCTGGGCAGATCGGAAGAGCACACGTC2846030.2076820079004439No Hit
GCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCCAGATCGGAAGAGC2627110.19170686176018353No Hit
GGGAGACCGGGCTTGGACCATGTCTCCCGAACAATCAGATCGGAAGAGCA2612820.19066408431479562No Hit
TGGCCGAGGGCACGTCTGCCTGGGTGTCACAAGATCGGAAGAGCACACGT2566420.1872781589497852No Hit
GGCCCCTCCTTTTGGCTCTAAGGCCCGAGTAGATCGGAAGAGCACACGTC2542740.1855501694531592No Hit
GCGCTCTTAGTTCAGTTCGGTAGAACGCGGGAGATCGGAAGAGCACACGT2474460.18056760514447576No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTAGATCGGAAGAGCACACGTCT2442790.1782565651377973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTTGAGATCGGAAGAGCA2436030.17776327083892943No Hit
TTGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACACG2419760.1765760077852932No Hit
AGCGCGCGACCCACACCAGGCCATCTGGGCGAGATCGGAAGAGCACACGT2299350.16778938551803235No Hit
CGCCCGCCGCCCGCCCCGACCCACGTTAGGGGCGAGATCGGAAGAGCACA2236920.16323371050644614No Hit
TCCGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG2208920.16119047968273295No Hit
GACTGTTGTCGGCCGCGCCGGCGGCCAAAGCCAGATCGGAAGAGCACACG2121510.15481195088627692No Hit
TCCGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT1963600.14328885876582875No Hit
GTTGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACAC1930170.14084938710737405No Hit
GGGAGAGTGACACCCAGGCAGACGTGCCCTCAGATCGGAAGAGCACACGT1886630.13767216317650002No Hit
AGGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTTGAGATCGGAAGAGC1864390.13604925412223642No Hit
AGAACGTGAAACCGTGCTGAGCTCCCAAAGATCGGAAGAGCACACGTCTG1819980.13280854409076848Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATCGGGCGGCCGCGCCGTCCGAATTGTAGTCTGAGATCGGAAGAGCAC1771340.129259160259861No Hit
CCGTTGTAGTCTAGCTGGTTAGGATACTCGGAGATCGGAAGAGCACACGT1736830.12674087826963454No Hit
GGACTGTTGTCGGCCGCGCCGGCGGCCAAAGCCAGATCGGAAGAGCACAC1727830.12608412550486955No Hit
CCGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC1718150.12537775142010013No Hit
AAGTCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACG1655820.12082937366145578No Hit
CGCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGAGATCGGAAGAGCA1577610.11512219213564835No Hit
CGCGCGTGCCCCGAAAGGGAATCGGGTTAAGATAGATCGGAAGAGCACAC1576390.11503316564975799No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC1571680.11468946503619766TruSeq Adapter, Index 3 (100% over 50bp)
GCCCCTCCTTTTGGCTCTAAGGCCCGAGTAGATCGGAAGAGCACACGTCT1562880.11404730677731638Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCCCCGGCAGCCGTCGTCGGCGCAGCCGGTCACAGATCGGAAGAGCA1525510.11132032335295346No Hit
TCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGTCT1435410.1047454984523621Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCTCTTAGTTCAGTTCGGTAGAACGCGGGTAGATCGGAAGAGCACACG1409520.1028562396657216No Hit
TGTTGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACA1387250.10123114143557545No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)