FastQCFastQC Report
Tue 25 Jul 2023
SRR8594312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8594312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120424619
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG117503299.757414304129956TruSeq Adapter, Index 5 (100% over 49bp)
CGGCCGAGGGCACGCCTGCCTGGGCGTCACGCAGATCGGAAGAGCACACG34255392.844550415393052No Hit
AGCCGTCGTCGGCGCAGCCGGTCACCGCGCGAGATCGGAAGAGCACACGT18351861.523929255694801No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCCGAGATCGGAAGAGCA16454961.3664116304989098No Hit
TCATGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACG14572331.2100789789503092No Hit
AGTCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGT14099221.1707921616924526No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT11781810.9783555968734268TruSeq Adapter, Index 5 (100% over 50bp)
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGGAGATCGGAAGAGCACACG10608790.8809486040391791No Hit
AGCGCGCGACCCACACCAGGCCATCTGGGCGAGATCGGAAGAGCACACGT9100430.7556951456910982No Hit
CGCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCAGATCGGAAGAGC8826130.7329174111815127No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC8642180.7176422953848No Hit
TCATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT8052600.6686838677064861No Hit
AGCGCGCGACCCACACCAGGCCATCTGGGCAGATCGGAAGAGCACACGTC7975350.6622690664273557No Hit
GACTGTTGTCGGCCGCGCCGGCGGCCAAAGCCAGATCGGAAGAGCACACG7359840.6111574245462217No Hit
GCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCAGATCGGAAGAGCA6769830.5621632898834416No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCCAGATCGGAAGAGCAC5859170.4865425399435974No Hit
CGCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCCAGATCGGAAGAG5164920.42889236793018215No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC5086020.42234055147809935TruSeq Adapter, Index 5 (100% over 50bp)
AAGTCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACG4698140.39013119069946983No Hit
TCCGATGATTCATGATAACTCGACGGATCGCACAGATCGGAAGAGCACAC4217630.3502298811507969No Hit
AGAACGTGAAACCGTGCTGAGCTCCCAAGCAGATCGGAAGAGCACACGTC4079360.33874800965739404No Hit
CGCGCGTGCCCCGAAAGGGAATCGGGTTAAGATAGATCGGAAGAGCACAC3999980.33215633424590696No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTAGATCGGAAGAGCACACGTCT3675440.3052066953186707Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCAGATCGGAAGAGCACA3542480.29416576356367796No Hit
ACGCGCGTGCCCCGAAAGGGAATCGGGTTAGATCGGAAGAGCACACGTCT3497860.29046054112905273Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGACCGGGCTTGGACCATGTCTCCCGAACAATAGATCGGAAGAGCAC3420210.2840125240504186No Hit
GGGAGACCGGGCTTGGACCATGTCTCCCGAACAATCAGATCGGAAGAGCA3276130.2720481930692261No Hit
GACTGTTGTCGGCCGCGCCGGCGGCCAAAGCAGATCGGAAGAGCACACGT3239040.2689682580602559No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG3078570.25564290969440395TruSeq Adapter, Index 5 (100% over 50bp)
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCCGTAGATCGGAAGAGC3064400.2544662399969893No Hit
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGTC2840820.2359002688644587No Hit
CCGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC2718380.22573291263641035No Hit
TCCGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGT2596460.21560873694771665No Hit
GCTTTCGTAGCTCAGTTGGTTAGAGCACCCGTTAGATCGGAAGAGCACAC2476720.20566558736631754No Hit
GGACTGTTGTCGGCCGCGCCGGCGGCCAAAGCCAGATCGGAAGAGCACAC2457660.20408285451997155No Hit
GCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCCAGATCGGAAGAGC2393650.19876749620440984No Hit
TCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGTCT2258600.18755301189701085Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACG2190010.18185733267713305No Hit
AAGCGCGCGACCCACACCAGGCCATCTGGGCGAGATCGGAAGAGCACACG1979650.16438914371819602No Hit
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTTGAGATCGGAAGAGCA1979340.16436340147358075No Hit
GGGAGAGTGACACCCAGGCAGACGTGCCCTCAGATCGGAAGAGCACACGT1963780.16307130687289118No Hit
CTAAGTCAGAATCCAAGCTAGCAAGCGGCGCCTAGATCGGAAGAGCACAC1868810.15518504567575173No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCAGATCGGAAGAGCACACG1862150.15463200261401697No Hit
GCGCTCTTAGTTCAGTTCGGTAGAACGCGGGAGATCGGAAGAGCACACGT1852860.15386056567054615No Hit
TTGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACACG1741310.14459750958398299No Hit
CCGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT1724260.14318168613014254No Hit
GCCCGCCGCCCGCCCCGACCCACGTTAGGGGCGAGATCGGAAGAGCACAC1681200.13960600531358128No Hit
GGCGCGTCGAGAGAACCATGTCGAAGGAACAGATCGGAAGAGCACACGTC1664410.13821177212941815No Hit
TGGCCGAGGGCACGTCTGCCTGGGTGTCACAAGATCGGAAGAGCACACGT1626380.1350537799916145No Hit
GCGCGTCGAGAGAACCATGTCGAAGGAACAGATCGGAAGAGCACACGTCT1562470.1297467256259287Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCAGTAAAACGCGGCGTGTTCGAATGAGATCGGAAGAGCACACGTCTG1534310.1274083333408761Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAAGATCGGAAGAGCA1532310.12724225434335815No Hit
GTTGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACAC1520350.12624910193820085No Hit
TCATGCATTGGATGGATGCCCGGGCATTGAAGATCGGAAGAGCACACGTC1475840.12255301384843909No Hit
AAGCGCGCGACCCACACCAGGCCATCTGGGCAGATCGGAAGAGCACACGT1462940.12148180431444836No Hit
CAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGTCTG1458560.12111809130988407Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGGCGGGCATGCCTGTTCGAGCGTCATTAGATCGGAAGAGCACACGTC1449650.12037820937594165No Hit
CATGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACGT1416040.11758725182265264No Hit
ATCATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACG1349490.11206097318024315No Hit
GGGAGACCGGGCTTGGACCATGTCTCCCGAACAGATCGGAAGAGCACACG1348060.11194222669701782No Hit
CGCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGAGATCGGAAGAGCA1345950.11176701335463639No Hit
GTCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGTC1324830.11001321914084694No Hit
CTGCCACGATCCACTGAGATCCAGCCCCGCAGATCGGAAGAGCACACGTC1291380.10723554790735938No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGGCAGATCGGAAGAGCACAC1290040.10712427497902237No Hit
AGCGCGCGACCCACACCAGGCCATCTGGGCGAAGATCGGAAGAGCACACG1271690.10560050017679523No Hit
CGCGCGTGCCCCGAAAGGGAATCGGGTTAAGAAGATCGGAAGAGCACACG1236930.10271404719993343No Hit
CGCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCCGAGATCGGAAGA1230450.1021759512479753No Hit
TGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAAGATCGGAAGAGC1229600.10210536767403017No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)