FastQCFastQC Report
Tue 18 Jul 2023
SRR8594313_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8594313_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203946105
Total Bases14.7 Gbp
Sequences flagged as poor quality0
Sequence length50-75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCTAGATCGGAAGAGCACAC43025582.1096544109042927No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCCAGATCGGAAGAGCAC39049241.9146842740634835No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCAGATCGGAAGAGCACA36489261.7891618964726No Hit
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTTGAGATCGGAAGAGCA23796391.1667979636090624No Hit
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAATAGATCGGAAGAGCACAC21235731.0412422438761455No Hit
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGTC19345050.9485373599069225No Hit
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAAAGATCGGAAGAGCACACG18811050.9223539718986052No Hit
CCGATGATTCATGATAACTCGACGGATCGCAGATCGGAAGAGCACACGTC15521160.7610422371145553No Hit
TTCGGTTCCTATCTACCGTTGGTGTTAAAGATCGGAAGAGCACACGTCTG13151460.6448497753855118Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCACCACCAGGCGTGGAGCCTGCGGCTTAATAGATCGGAAGAGCACA13127470.64367348422761No Hit
TGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACACGT13091790.6419240024221105No Hit
GCGCCCCCGGCAGCCGTCGTCGGCGCAGCCGGTCAGATCGGAAGAGCACA12520960.6139347451622085No Hit
TCGGTTCCTATCTACCGTTGGTGTTAAAGATCGGAAGAGCACACGTCTGA10914620.535171779818987Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAAGATCGGAAGAGCACACGT10669540.5231548795697766No Hit
CCTCCGCAGCTAGCTTCTTAGAGGGACTATGAGATCGGAAGAGCACACGT10341850.507087399389167No Hit
ACCGTCCACGCCCGGTCGTACTCATAACCAGATCGGAAGAGCACACGTCT10170520.49868665057368955Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCACCACCAGGCGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGT9345990.45825783238174617No Hit
CGCGGTGTCCGGTGCGCCCCCGGCGGCCAGATCGGAAGAGCACACGTCTG9177690.45000565222856304Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTAGATCGGAAGAGCACA8748340.42895352181401064No Hit
AGTCAGAATCCAAGCTAGCAAGCGGCGCCTGAGATCGGAAGAGCACACGT8368490.41032850320921793No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGCAGATCGGAAGAGCACACG8353260.40958173729280095No Hit
CCCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCCGAGATCGGAAGAGCA8157930.40000420699380357No Hit
TGCTTAACGGCCCGCCAACCCTGGAAAGATCGGAAGAGCACACGTCTGAA7793390.382129876910373Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAGGGCACGCCTGCCTGGGCGTCACGCAGATCGGAAGAGCACACGTCTG7754990.3802470265367412Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCACCACCAGGCGTGGAGCCTGCGGCTTAAAGATCGGAAGAGCACAC7018570.3441384673661701No Hit
AGGTCCAGACATAGCAAGGATTGACAGAAGATCGGAAGAGCACACGTCTG6923200.33946223194603303Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTATGCCTGAGCGGGGCGAAGCCAGAGAGATCGGAAGAGCACACGTCTG6733850.3301779163666793Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCGCAGCTAGCTTCTTAGAGGGACTATAGATCGGAAGAGCACACGTC6375810.3126222979350354No Hit
AACCGGGAGATGCCCAGCTTGAGAATCGGAGATCGGAAGAGCACACGTCT6160440.3020621550972989Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTCGGTTCCTATCTACCGTTGGTGTTAAAGATCGGAAGAGCACACGTCT5961850.29232477864679Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGATACTCGGCTCTCACCCGAGAGACCCGGGTAGATCGGAAGAGCACA5893210.2889591836039232No Hit
GCCATGCCCCGATGAGTAGGAGGGCGCGGCGGCCGCAGATCGGAAGAGCA5882370.2884276706338667No Hit
AGGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTTGAGATCGGAAGAGC5595580.27436562223142236No Hit
CCTCCGCAGCTAGCTTCTTAGAGGGACTATGGAGATCGGAAGAGCACACG5555490.27239990682832604No Hit
CCTCCGCAGCTAGCTTCTTAGAGGGACTATGGCAGATCGGAAGAGCACAC5446750.26706810605674475No Hit
AGTTCGGTTCCTATCTACCGTTGGTGTTAAAGATCGGAAGAGCACACGTC5320500.2608777451278121No Hit
CTGGGCCGCACGCGCGCTACACTGATGTAGATCGGAAGAGCACACGTCTG5223970.25614463193597153Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCACCACCAGGCGTGGAGCCTGCGGCTTAAGATCGGAAGAGCACACG5077470.24896136163031893No Hit
CCGATGATTCATGATAACTCGACGGATCGCAAGATCGGAAGAGCACACGT5023450.246312622641163No Hit
TGGGCCGCACGCGCGCTACACTGATGTATTAGATCGGAAGAGCACACGTC4826150.23663849819539334No Hit
TGGGAAATTTCATCGTGATGGGGATAGATCAAGATCGGAAGAGCACACGT4822130.23644138729690375No Hit
AAGCCCGATCCAGCAATATCGCGTGAGTGAAGATCGGAAGAGCACACGTC4766700.23372351239559097No Hit
CCTCCGCAGCTAGCTTCTTAGAGGGACTAGATCGGAAGAGCACACGTCTG4583650.22474810195566128Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCAGTAAAACGCGGCGTGTTCGAATGAGATCGGAAGAGCACACGTCTG4539680.22259214021272924Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGCGCTACACTGATGTATTCAACGAGATCGGAAGAGCACACGTCTGAA4527450.22199247198175223Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTTGAAGATCGGAAGAGC4491920.22025034506052468No Hit
AGGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTAGATCGGAAGAGCAC4472930.21931921671168958No Hit
AGGTCCAGACATAGCAAGGATTGACAGACAGATCGGAAGAGCACACGTCT4459220.21864698028922885Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCAGTAAAACGCGGCGTGTTCGAATGAGATCGGAAGAGCACACGTCTGA4438570.21763445788778366Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCAGTAAAACGCGGCGTGTTCGAAAGATCGGAAGAGCACACGTCTGAA4415060.2164817023595523Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGAAGAAACTTACGAGGATTCCCCAGATCGGAAGAGCACACGTCTGAA4302090.2109424938515006Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGGCCGCACGCGCGCTACACTGATGTATTAGATCGGAAGAGCACACGT4219860.20691054629359065No Hit
TTGATCCTTCGATGTCGGCTCTTCCTATAGATCGGAAGAGCACACGTCTG4177050.20481146232236208Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCGGGAGATGCCCAGCTTGAGAATCGGGAGATCGGAAGAGCACACGTC3973660.19483872957514928No Hit
AGCGCGCGACCCACACCAGGCCATCTGGGCAGATCGGAAGAGCACACGTC3880280.1902600689530207No Hit
GCGCGTGCCCCGAAAGGGAATCGGGTTAAGATAGATCGGAAGAGCACACG3848180.18868612371881285No Hit
CGAGTTCTATCGGGTAAAGCCAATGATTAGAAGATCGGAAGAGCACACGT3808940.18676208599325786No Hit
CTGGGCCGCACGCGCGCTACACTGATGTATTCAGATCGGAAGAGCACACG3802690.18645563248192457No Hit
TGGGAAATTTCATCGTGATGGGGATAGATCAGATCGGAAGAGCACACGTC3750530.18389809405774138No Hit
CTAAGTCAGAATCCAAGCTAGCAAGCGGCGCCTAGATCGGAAGAGCACAC3720840.18244231729750368No Hit
AGGATACTCGGCTCTCACCCGAGAGACCCGGGTAGATCGGAAGAGCACAC3648860.17891295349818032No Hit
GATGTCGGCTCTTCCTATCATTGTGAAAGATCGGAAGAGCACACGTCTGA3615090.17725712388574422Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTCTGGCCAATGGAACAATGTAGGCAAGGGAGATCGGAAGAGCACAC3592690.17615879450112568No Hit
CAGGCGTGGAGCCTGCGGCTTAATTTGAAGATCGGAAGAGCACACGTCTG3496500.17144235238030164Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCAGAATCCAAGCTAGCAAGCGGCGCCTAGATCGGAAGAGCACACGTC3486510.1709525170877865No Hit
TGGGCCGCACGCGCGCTACACTGATGTATTCAGATCGGAAGAGCACACGT3417520.167569760648285No Hit
GTCGATGGCCCGAGGTAGAGCACTCAATGGGAGATCGGAAGAGCACACGT3386700.16605857709319824No Hit
GCGCCCCCGGCAGCCGTCGTCGGCGCAGCCGGAGATCGGAAGAGCACACG3368410.16516177153763245No Hit
AAAGCCCGATCCAGCAATATCGCGTGAGTGAAGATCGGAAGAGCACACGT3335160.1635314388573393No Hit
TCATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT3103280.15216176842406476No Hit
ATCACCAGCTAAGGCCCCTAAATGACAGATCGGAAGAGCACACGTCTGAA3091530.1515856358227582Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGCTTAACGGCCCGCCAACCCTGGAAAGATCGGAAGAGCACACGTCTGA3057060.14989548341705275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTCCTAAGATGAGCTCAACGAGAACAGATCGGAAGAGCACACGTCTGAA3041910.14915264010558082Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATGTCGGCTCTTCCTATCATTGTGAAGAGATCGGAAGAGCACACGTCTG3029920.14856473968943903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCCACGATCCACTGAGATCCAGCCCCGCAGATCGGAAGAGCACACGTCT3010580.14761644994396925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACCGGGAGATGCCCAGCTTGAGAATCGGGAGATCGGAAGAGCACACGT3010330.14760419180351594No Hit
CCGATGATTCATGATAACTCGACGGATCGAGATCGGAAGAGCACACGTCT2944850.1443935396559792Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCACCACCAGGCGTGGAGCCTGCGGCTTAATTTGAGATCGGAAGAGC2943830.14434352644292964No Hit
GAACCGGGAGATGCCCAGCTTGAGAATCGGAGATCGGAAGAGCACACGTC2716830.13321313491130415No Hit
CCTCCGCAGCTAGCTTCTTAGAGGGACTAAGATCGGAAGAGCACACGTCT2655860.13022361961754553Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCGCGACCCACACCAGGCCATCTGGGCGAGATCGGAAGAGCACACGT2553440.12520170463662447No Hit
TTGTCGGCCGCGCCGGCGGCCAAAGCCCGGGGAGATCGGAAGAGCACACG2547030.12488740591540104No Hit
ACTATGCCTGAGCGGGGCGAAGCCAGAAGATCGGAAGAGCACACGTCTGA2539420.1245142681200016Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCCCGATCCAGCAATATCGCGTGAGTGAAAGATCGGAAGAGCACACGT2518040.12346595194843266No Hit
TTGATCCTTCGATGTCGGCTCTTCCTATCAGATCGGAAGAGCACACGTCT2483030.12174932195934804Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTATGCCTGAGCGGGGCGAAGCCAGAGAGATCGGAAGAGCACACGTCT2370510.11623217810411235Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCGCACGCGCGCTACACTGATGTATTCAGATCGGAAGAGCACACGTC2370400.11622678452231289No Hit
ACCGGGAGATGCCCAGCTTGAGAATCGGAGATCGGAAGAGCACACGTCTG2363450.11588600821771027Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGGTAAGCCGATCCTAAGGGACGGGGTAGATCGGAAGAGCACACGTCT2354480.1154461861382447Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGAGGTAGGGTCCAGCGGCCGGAAGAGCAGATCGGAAGAGCACACGTC2310960.11331228904812866No Hit
CCTCCGCAGCTAGCTTCTTAGAGGGAAGATCGGAAGAGCACACGTCTGAA2291190.11234291530107918Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCACCACCAGGCGTGGAGCCTGCGGCTTAATAGATCGGAAGAGCACA2285190.11204871993019921No Hit
CCGGAGGTAGGGTCCAGCGGCCGGAAGAGCAAGATCGGAAGAGCACACGT2280100.11179914419056937No Hit
CCGTCCAAGGCTAAATACAGGCGAGAGACCGAAGATCGGAAGAGCACACG2252970.11046889078857376No Hit
ACGCGCGCTACACTGATGTATTCAACGAGATCGGAAGAGCACACGTCTGA2247400.11019577941927354Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTAGCTTCTTAGAGGGACTATGGCCAGATCGGAAGAGCACACGTCTGA2231540.10941812298891417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGGGATCAGCCCATCACGTAGGATCAGATCGGAAGAGCACACGTCTGA2177650.10677575823279391Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG2173030.10654922779721632TruSeq Adapter, Index 7 (100% over 49bp)
ACGAGTTCTATCGGGTAAAGCCAATGATTAGAAGATCGGAAGAGCACACG2129690.10442415656822669No Hit
CGGAGGTGTCCAAAGGTCAGCTCAGTGTGGACAGATCGGAAGAGCACACG2100030.10296985078484337No Hit
GAACCCAGAGCTAGCTGGTTCTCCCCGAAATAGATCGGAAGAGCACACGT2077620.10187103107460671No Hit
GGGGCACCACCAGGCGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGT2060290.10102129677838172No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)