FastQCFastQC Report
Fri 14 Jul 2023
SRR8649954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8649954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38303136
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG9399102.453872184251441No Hit
ACGGAGTCGAGCTCAACCCGCCTGTAGGCACCATCAATAGATCGGAAGA3811840.9951770006508083No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCA3323350.8676443620699883No Hit
ACCCCTCCCACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA3141760.8202357112482905No Hit
GTGGGGGGGCCGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2962500.7734353657100035No Hit
ACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAG2801400.7313761463291152No Hit
ATGTACACGGAGTCGAGCTCCTGTAGGCACCATCAATAGATCGGAAGAG2126290.5551216485250712No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGAT2041270.5329250325613026No Hit
ACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAG1869680.4881271340289213No Hit
ACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1740390.4543727176803487No Hit
ATGTACACGGAGTCGACCCCTGTAGGCACCATCAATAGATCGGAAGAGC1720880.44927913996389224No Hit
ATGTACACGGAGTCGACCCAACGCCTGTAGGCACCATCAATAGATCGGA1378210.3598164912658849No Hit
TTGGGAATACCGGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1099090.2869451733664836No Hit
ACGGCGCGACCGCTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1013700.2646519595680103No Hit
ACGGAGTCGACCCAACGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1007670.2630776759375525No Hit
CTGGGAATACCGGGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA854270.22302873581943788No Hit
ATGTACACGGAGTCGACCCAACCTGTAGGCACCATCAATAGATCGGAAG800430.208972445493758No Hit
ACGGAGTCGAGCTCAACCCCTGTAGGCACCATCAATAGATCGGAAGAGC730170.1906292999090205No Hit
ACGGAGTCGAGCTCAACCTGTAGGCACCATCAATAGATCGGAAGAGCAC717040.187201382153148No Hit
CGGGGGGGATGCGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA692970.18091730139276327No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGATC647620.16907753976071307No Hit
ATGTACACGGAGTCGAGCTCAACCCGCCTGTAGGCACCATCAATAGATC631600.1648951145932281No Hit
CGTGGGGGGGCCGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC623870.16287700307358643No Hit
TACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAA614220.16035762711439605No Hit
ACACGGAGTCGAGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA606780.1584152274111446No Hit
ACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA598220.1561804234514897No Hit
TCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATAGATCGGAAGA590450.1541518689226908No Hit
TGTGGGGGGGCCGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC590020.1540396065742502No Hit
ACGGAGTCGAGCTCAACCCGCAACGCCTGTAGGCACCATCAATAGATCG548290.14314493727093258No Hit
ACGGAGTCGAGCTCAACCCTGTAGGCACCATCAATAGATCGGAAGAGCA536310.14001725602833145No Hit
ACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAG533020.13915831852514635No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCCTGTAGGCACCATCAATAG527260.13765452520650007No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCTGTAGGCACCATCAATAGATCG521980.13627604799774098No Hit
AGCGCCGCGGAGCCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA516310.13479575144969852No Hit
ACGGAGTCGACCCAACCTGTAGGCACCATCAATAGATCGGAAGAGCACA490270.12799735248831845No Hit
TCAACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAGCACA482980.12609411406940677No Hit
ATGTACACGGAGTCGAGCTCAACCTGTAGGCACCATCAATAGATCGGAA438590.11450498465713095No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAG435540.11370870520888941No Hit
CGTGGGGGGGCCGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA428200.11179241302853113No Hit
CTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA419060.10940618543609588No Hit
ACGGCGCGACCGCTCTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC412500.1076935319343043No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCCTGTAGGCACCATCAATAGATC412490.10769092118201495No Hit
TGTGGGGGGGCCGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA408380.10661790199110589No Hit
GTGGGGGGGCCGGGCCACCCCTCCCCTGTAGGCACCATCAATAGATCGG404180.10552138602959299No Hit
ATGTACACGGAGTCGAGCTCAACCCTGTAGGCACCATCAATAGATCGGA403830.1054300096994669No Hit
ACCGCCCGTCCCCGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC393140.1026391155021876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)