FastQCFastQC Report
Mon 17 Jul 2023
SRR8649955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8649955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45200648
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAG4115610.910520132366244No Hit
ACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA3455530.7644868277109655No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG2632470.5823965178552308No Hit
TCGGCGGGGGACCGTCCCCCGACCGGCCTGTAGGCACCATCAATAGATC2583140.5714829575009632No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCCTGTAGGCACCATCAATAGATCG1857500.4109454360034838No Hit
ACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1723240.3812423220127287No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAG1559390.34499284169554384No Hit
ACGGAGTCGAGCTCAACCCGCCTGTAGGCACCATCAATAGATCGGAAGA1490260.32969881316745725No Hit
TACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGA1438520.3182520746162754No Hit
TAGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATA1285460.28438972821805564No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCA1277500.28262869151787384No Hit
AAATTGGAATGAGTCCACTTTCTGTAGGCACCATCAATAGATCGGAAGA1180510.26117103453915086No Hit
TCGGGTTGCTTGGGAATGCAGCCCAAAGCCTGTAGGCACCATCAATAGA1158780.2563635813362676No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAAT1056160.23366036699296877No Hit
TAAGCAGGGTCGGGCCTGGTTCTGTAGGCACCATCAATAGATCGGAAGA1018400.22530650445542286No Hit
ACCCCTCCCACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1018210.22526446965981547No Hit
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGA950680.21032441835789611No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCCTGTAGGCACCATCAATA936130.2071054379574381No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA856310.18944639908702193No Hit
AGAGCAGCTCCCTCGCTGCGATCTCTGTAGGCACCATCAATAGATCGGA850920.18825393830637116No Hit
TTACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAG783380.17331167464678826No Hit
ACTACTCTGATCGTTTTTTCCTGTAGGCACCATCAATAGATCGGAAGAG774150.17126966852333622No Hit
ACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAG767210.169734292304836No Hit
AAATCGGTCGTCCGACCTGGGTCTGTAGGCACCATCAATAGATCGGAAG764430.16911925687437046No Hit
ATGGTTCCTTTGGTCGCTCGCTGTAGGCACCATCAATAGATCGGAAGAG739690.16364588401476013No Hit
ATGGTTCCTTTGGTCGCTCGCCTGTAGGCACCATCAATAGATCGGAAGA720970.1595043504686039No Hit
ATGAATGGATGAACGAGATTCCCCTGTAGGCACCATCAATAGATCGGAA716030.15841144578281266No Hit
ATGTACACGGAGTCGAGCTCCTGTAGGCACCATCAATAGATCGGAAGAG698560.15454645694459956No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG689560.15255533504740906No Hit
AGGACCCGAAAGATGGTGAACTCTGTAGGCACCATCAATAGATCGGAAG671150.1484823845888227No Hit
AGGATAGCTGGCGCTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG666480.1474492135599472No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT659970.1460089687209794No Hit
ACGAGAGGAACCGCAGGTTCAGACCTGTAGGCACCATCAATAGATCGGA629430.13925242841651295No Hit
AAGCAGGGTCGGGCCTGGTTCTGTAGGCACCATCAATAGATCGGAAGAG615990.1362790197167085No Hit
ACTTTGAAAAAATTAGAGTGTTCCTGTAGGCACCATCAATAGATCGGAA610760.13512195665867446No Hit
ACGTTCGTGGGGAACCTGGCGCTCTGTAGGCACCATCAATAGATCGGAA608150.1345445313084892No Hit
TATGGTTCCTTTGGTCGCTCGCTGTAGGCACCATCAATAGATCGGAAGA605880.1340423261188645No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCCTGTAGGCACCATCAATAGATCGG597640.1322193434041034No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGAT594380.13149811480578774No Hit
TCCTACTTGGATAACTGTGGTCTGTAGGCACCATCAATAGATCGGAAGA590190.13057113694476238No Hit
ACGACTCTTAGCGGTGGATCCTGTAGGCACCATCAATAGATCGGAAGAG586990.12986318249242798No Hit
TAGGGTCGGGCCTGGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC577440.1277503809237425No Hit
AAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATAGATCGGAAGA573430.12686322550066095No Hit
TGTGGGGGGGCCGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC572720.12670614810654926No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCCTGTAGGCACCATCAAT564840.12496281026767582No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAG563720.12471502620935877No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC559130.12369955404179162No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGATC547270.12107569785282724No Hit
ATGTACACGGAGTCGACCCCTGTAGGCACCATCAATAGATCGGAAGAGC532010.11769964005825759No Hit
TTGGCGCTAAACCATTCGTCTGTAGGCACCATCAATAGATCGGAAGAGC531670.11762441989769705No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAA529710.11719079779564223No Hit
ACGGAAGACCCGCTCCCCGGCGCCTGTAGGCACCATCAATAGATCGGAA527760.11675938805125094No Hit
ACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGGAAG525220.11619744920471052No Hit
ACCTGGTTGATCCTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC521750.11542976109546041No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT520200.11508684565761092No Hit
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCCTGTAGGCACCATCAA516380.11424172503013673No Hit
AGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATAGATCGGA509490.11271741059995424No Hit
ACTGATGATGTGTTGTTGCCCTGTAGGCACCATCAATAGATCGGAAGAG497440.1100515196153825No Hit
TATGGTTCCTTTGGTCGCTCGCCTGTAGGCACCATCAATAGATCGGAAG494680.10944090890024408No Hit
AACTGGCTTGTGGCGGCCAAGCCTGTAGGCACCATCAATAGATCGGAAG475200.10513123617165843No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTCTGTAGGCACCATCAA458340.10140120115092156No Hit
TCGCGGCGCCTCGCCTCGGCCGGCGCCTCTGTAGGCACCATCAATAGAT457350.1011821777422306No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT452470.10010254720242064No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)