FastQCFastQC Report
Mon 17 Jul 2023
SRR8649956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8649956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45086750
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAAT3676840.8155034461343965No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG3060790.6788668511258851No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATC2501180.5547483462436303No Hit
ACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA1648760.3656861494785053No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGA1303680.28914925116580814No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCCTGTAGGCACCATCAATA1291980.286554253744171No Hit
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGA1252930.2778931726061426No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1213550.269158899233145No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAA1151170.25532334887744185No Hit
TAGACGACCTGCTTCTGGGTCGGGGTTTCGTCTGTAGGCACCATCAATA1139730.2527860180651743No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCG1076720.23881073707907535No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA986300.2187560646974998No Hit
TCGGCGGGGGACCGTCCCCCGACCGGCCTGTAGGCACCATCAATAGATC982300.2178688860918119No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA915560.20306631105590892No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT900530.1997327374450365No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAA847850.18804859520812656No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCCTGTAGGCACCATCAATAGATCGG821330.1821666010524156No Hit
TCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCCTGTAGGCACCATCAATA736050.1632519531791491No Hit
TCGGGTTGCTTGGGAATGCAGCCCAAAGCCTGTAGGCACCATCAATAGA678000.1503767736641031No Hit
ATGAATGGATGAACGAGATTCCCCTGTAGGCACCATCAATAGATCGGAA668700.14831408340587868No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA656710.14565476553532913No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC648350.14380056224944135No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAAT615250.1364591592873738No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCCTGTAGGCACCATCAATAGA607150.13466262261085574No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT605600.13431884090115168No Hit
TATTGGAGGGCAAGTCTGGTGCCAGCCTGTAGGCACCATCAATAGATCG587370.13027552440572895No Hit
ACGAGAGGAACCGCAGGTTCAGACCTGTAGGCACCATCAATAGATCGGA579240.12847233388966825No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG571670.12679334837840386No Hit
ACGGAAGACCCGCTCCCCGGCGCCTGTAGGCACCATCAATAGATCGGAA564190.12513432438576744No Hit
AGAATCCCGCCCAGGCGGAACGATCTGTAGGCACCATCAATAGATCGGA556900.1235174413769012No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT552310.1224994039268743No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGATCTGTAGGCACCATCAATAG542840.12039900857790814No Hit
AGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCAATAGATCG540340.11984452194935319No Hit
AGGACCCGAAAGATGGTGAACTCTGTAGGCACCATCAATAGATCGGAAG536360.11896177923669371No Hit
ACTTTGAAAAAATTAGAGTGTTCCTGTAGGCACCATCAATAGATCGGAA532050.11800584428906497No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCA520640.11547516731634017No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCCTGTAGGCACCATCAATAGAT519740.1152755521300604No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG498400.11054245426871531No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAG485870.10776336728639789No Hit
ACGGGGTCGGCGGCGACGTCGGCTACCCCTGTAGGCACCATCAATAGAT484940.10755709826057544No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCTGTAGGCACCATCAATAGATCGG482480.10701148341807738No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCCTGTAGGCACCATCAATAG480380.10654571465009122No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA468340.10387530704697057No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA467940.10378658918640178No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC460050.10203662938668234No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCG457470.10146439918601363No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)