FastQCFastQC Report
Fri 14 Jul 2023
SRR8649960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8649960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44808546
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCCTCCCACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2964470.6615858501634934No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCCTGTAGGCACCATCAATAGATCG2754750.6147822783627034No Hit
ACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGAG1340820.29923309718641616No Hit
TGTGGGGGGGCCGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1189310.26542035084110965No Hit
ACGGAGTCGAGCTCAACCCGCCTGTAGGCACCATCAATAGATCGGAAGA1147540.25609846835913846No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG1132580.25275981952192783No Hit
ACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGAA1049920.23431244566605663No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCCTGTAGGCACCATCAATA1003120.22386800946408747No Hit
GTGGGGGGGCCGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA980920.21891359741956365No Hit
CGTGGGGGGGCCGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC840430.18756020335942167No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGATC777960.17361866640350257No Hit
TGTGGGGGGGCCGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA767920.171378022397781No Hit
TCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATCG617440.13779514291760328No Hit
CGGGGGGGATGCGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA616590.13760544696094357No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAG610650.13627980698146286No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCTGTAGGCACCATCAATAGATCGG581160.12969847314393998No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC564180.12590901744502042No Hit
ACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA561220.12524842917241724No Hit
AACTGGCTTGTGGCGGCCAAGCCTGTAGGCACCATCAATAGATCGGAAG552030.12319748112335535No Hit
TCGGCGGGGGACCGTCCCCCGACCGGCCTGTAGGCACCATCAATAGATC536340.11969591693513108No Hit
GTGGGGGGGCCGGGCCACCCCTCCCCTGTAGGCACCATCAATAGATCGG528320.11790607979111842No Hit
TCGTCGGGCCCGGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA522820.11667863536567331No Hit
AAATTGGAATGAGTCCACTTTCTGTAGGCACCATCAATAGATCGGAAGA503050.11226653058548251No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCCTGTAGGCACCATCAATAGATC501810.11198979766047308No Hit
TCGGGGGGGATGCGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC483950.10800395085348229No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCTGTAGGCACCATCAATAGATCG480580.10725186217825501No Hit
AGAATCCCGCCCAGGCGGAACCTGTAGGCACCATCAATAGATCGGAAGA468820.10462736282493969No Hit
TCGGGTTGCTTGGGAATGCAGCCCAAAGCCTGTAGGCACCATCAATAGA466760.10416762909468208No Hit
CGTGGGGGGGCCGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA456860.1019582291288809No Hit
AAATCGGTCGTCCGACCTGGGTCTGTAGGCACCATCAATAGATCGGAAG453560.10122176247361384No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)