FastQCFastQC Report
Fri 14 Jul 2023
SRR8649961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8649961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86205240
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCGAGGGGGGCCCGGGCCTGTAGGCACCATCAATAGATCGGAAGAGC2804320.32530737110644314No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG2753030.31935761677596397No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAAT2446300.2837762530444785No Hit
TACCTGGTTGATCCTGCCAGTCTGTAGGCACCATCAATAGATCGGAAGA2118790.24578436299231926No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATC1940100.22505592467464852No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG1902510.22069540088282338No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1664340.19306714997835397No Hit
TACCTGGTTGATCCTGCCAGTAGCCTGTAGGCACCATCAATAGATCGGA1591520.18461986765537686No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC1398250.16220011683744515No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA1342030.1556784715175087No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAAT1291830.1498551596167472No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC1269210.14723118919453157No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA1210960.14047405934952445No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG997200.11567742285735763No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA995110.11543497819854107No Hit
AGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGGAAG986550.11444199911745505No Hit
ATGTACACGGAGTCGACCCCTGTAGGCACCATCAATAGATCGGAAGAGC980690.11376222605493588No Hit
TCGGCGGCGGCGGCGGCGGGACTGTAGGCACCATCAATAGATCGGAAGA945020.10962442654298046No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA903130.10476509316603028No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)