FastQCFastQC Report
Fri 14 Jul 2023
SRR8668645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8668645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38726483
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA12828153.312500647166953Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG4799841.2394205794520508No Hit
AGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC4688861.2107631875582403No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATCTGTAGGCACCATCAAT2575330.665004875345897No Hit
TCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGAT2276140.5877476661126186No Hit
TGCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATCTGTAGGCACCATCAA1794150.463287616383858No Hit
GCCGCCTTAGCTCAGTCGGTAGAGCGATTCACCTGTAGGCACCATCAATA1485590.383610874243344No Hit
TGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGA1208550.31207326521233547No Hit
TGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1165480.3009516769183507No Hit
TGTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGAT1163760.30050753640602995No Hit
AGGTGCGGCTGGATCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAG1145600.29581823890385295No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCACTGTAGGCACCATCAATA1139910.29434896011600126No Hit
AGGTGCGGCTGGATCACCTCCTGTAGGCACCATCAATAGATCGGAAGAGC1120050.2892206865260654No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATC1033380.2668406526872063No Hit
ACGGGCGTGGGGGTTCAAGTCCCCCCTCGGACACCACTGTAGGCACCATC1017640.2627762505570155No Hit
AGGTGCGGCTGGATCACCTGTAGGCACCATCAATAGATCGGAAGAGCACA928020.23963446409528072No Hit
CTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG864290.22317802522888536No Hit
TGCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCACTGTAGGCACCATCAAT766590.19794981124415556No Hit
TTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATC747250.19295581269282833No Hit
GGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA734580.18968414973288436No Hit
ATGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG680160.17563175050003896No Hit
AGCGGTGGAATGCGCAGATATCAGGAGGCTGTAGGCACCATCAATAGATC667360.17232651878044283No Hit
GTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG656040.169403454478425No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC655970.16938537899245848Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGGGCGTGGCGAAATCGGTATACGCGCGGGTCTGTAGGCACCATCAATA590390.1524512308540902No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTAGCTGTAGGCACCATCAATAG581390.15012723980124917No Hit
TACGGGCGTGGGGGTTCAAGTCCCCCCTCGGACACCACTGTAGGCACCAT572780.14790395502736461No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGATCCGAGCTGTAGGCACCATCAA557990.14408486306386253No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGAT532340.13746148856326562No Hit
TCCTCTCGCCTCCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACA526910.1360593472947182No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCG487630.12591641745520757No Hit
TTAGCGGTGGAATGCGCAGATATCAGGAGGCTGTAGGCACCATCAATAGA482030.12447037857788429No Hit
GCCGCCTTAGCTCAGTCGGTAGAGCGATTCACTGTAGGCACCATCAATAG479450.12380416780940319No Hit
TGTGCGTCCATGATGAATCAGCGGTACTAACCCTGTAGGCACCATCAATA477690.12334969844795872No Hit
TTGGGGTATGGTGTAATTGGCAACACAGCTGACTGTAGGCACCATCAATA465430.12018390619153306No Hit
AGGTAGCCGTACCGGAAGGTGCGGCTGGATCACCCTGTAGGCACCATCAA459460.11864232545981518No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGCTGTAGGCACCATCAATAGATCG451750.1166514397912147No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATGGCTGTAGGCACCATCA446650.11533451152793814No Hit
AAGTCCCCCCTCGGACACCACTGTAGGCACCATCAATAGATCGGAAGAGC435530.11246309147153899No Hit
TATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG434140.1121041639644891No Hit
TGTGCGTCCATGATGAATCAGCGGTACTAACCACTGTAGGCACCATCAAT408600.10550919379898246No Hit
AGGTGCGGCTGGATCACCTCTGTAGGCACCATCAATAGATCGGAAGAGCA408040.10536458991125014No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCCTGTAGGCACCATCAATAG388740.10038092020904661No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)