FastQCFastQC Report
Fri 14 Jul 2023
SRR8668646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8668646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57296954
Total Bases8.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG8853261.5451536917651851No Hit
AGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC6089111.062728395649095No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5386440.9400918589843363Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATCTGTAGGCACCATCAAT3742790.6532266968327846No Hit
GCCGCCTTAGCTCAGTCGGTAGAGCGATTCACCTGTAGGCACCATCAATA3513170.6131512680412295No Hit
TGCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATCTGTAGGCACCATCAA3336850.5823782534757432No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCACTGTAGGCACCATCAATA2143780.37415252475724975No Hit
TGTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGAT1936240.3379307039602838No Hit
TGCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCACTGTAGGCACCATCAAT1821760.3179505842492081No Hit
TGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1760540.3072658975902977No Hit
CTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG1742110.30404932171437943No Hit
TTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATC1569080.2738505087024347No Hit
TCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGAT1552390.2709376138913074No Hit
ACGGGCGTGGGGGTTCAAGTCCCCCCTCGGACACCACTGTAGGCACCATC1432370.24999060159463277No Hit
TGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGA1303410.22748329693058378No Hit
AGGTGCGGCTGGATCACCTGTAGGCACCATCAATAGATCGGAAGAGCACA1268450.22138175093915113No Hit
AGGTGCGGCTGGATCACCTCCTGTAGGCACCATCAATAGATCGGAAGAGC1252290.21856135668224178No Hit
AGGTGCGGCTGGATCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAG1203370.21002338099857804No Hit
GCCGCCTTAGCTCAGTCGGTAGAGCGATTCACTGTAGGCACCATCAATAG1170430.2042743842892591No Hit
TACGGGCGTGGGGGTTCAAGTCCCCCCTCGGACACCACTGTAGGCACCAT1161860.2027786677804897No Hit
GCGGGCGTGGCGAAATCGGTATACGCGCGGGTCTGTAGGCACCATCAATA1128190.19690226464743657No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATC1074460.1875248028019081No Hit
GTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG1073850.18741833989988368No Hit
TTGGGGTATGGTGTAATTGGCAACACAGCTGACTGTAGGCACCATCAATA1045590.1824861405372439No Hit
ATGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG995110.17367589907135378No Hit
AGGTAGCCGTACCGGAAGGTGCGGCTGGATCACCCTGTAGGCACCATCAA906430.15819863652786847No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGAT805390.1405641912482817No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTAGCTGTAGGCACCATCAATAG783620.13676468735144282No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCCTGTAGGCACCATCAATAG781770.1364418080584179No Hit
TGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCG737880.12878171499308672No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGATCCGAGCTGTAGGCACCATCAA718450.1253906097695874No Hit
AAGTCCCCCCTCGGACACCACTGTAGGCACCATCAATAGATCGGAAGAGC686550.1198231235817527No Hit
TATGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCG672590.11738669388952158No Hit
GGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCACA669310.11681423762945582No Hit
TGCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCCTGTAGGCACCATCAATA663080.11572691979402606No Hit
TTAGCGGTGGAATGCGCAGATATCAGGAGGCTGTAGGCACCATCAATAGA650670.11356101059054552No Hit
TCCTCTCGCCTCCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACA641550.11196930294060659No Hit
ACAAGGTAGCCGTACCGGAAGGTGCGGCTGGATCACCCTGTAGGCACCAT623860.10888187878189824No Hit
ATCTCGGCTTGTTCGTGTGACCGGGAGCTGTAGGCACCATCAATAGATCG608480.10619761741610208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)