FastQCFastQC Report
Fri 14 Jul 2023
SRR8668647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8668647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30959438
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGAT17649355.700797927921044No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATC17589035.681314370112274No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCG10266823.3162165282199245No Hit
ATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA9481493.062552362869119No Hit
TACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAAT8982802.9014738575034857No Hit
ACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAATA7482672.416926948092533No Hit
TATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG5834371.8845206427842778No Hit
AGGACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCA4076541.3167357882917643No Hit
TACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAATA3769481.217554401342815No Hit
TACCCGTTAACTCCCTTTGGGGGTGAAGCGGCTGTAGGCACCATCAATAG3271571.0567278385350536No Hit
ACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAATAG3163121.021698132892464No Hit
ACCCGTTAACTCCCTTTGGGGGTGAAGCGGCTGTAGGCACCATCAATAGA2702210.872822691419657No Hit
CACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAAT2386150.7707342749567999No Hit
TTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGG2029300.655470554730354No Hit
TCAGCGGTACTAACCATCCAAAACCACCCTGTAGGCACCATCAATAGATC1870960.6043262154823352No Hit
TTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG1781170.5753237510319148No Hit
AAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA1743830.5632628085819904No Hit
TAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA1631420.5269540099532815No Hit
CATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG1538840.49705036635354943No Hit
AGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCG1513950.4890108147311977No Hit
TGGTGGGGCTGCATGGGACCTTCGTCTGTAGGCACCATCAATAGATCGGA1491080.4816237297330785No Hit
TTGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGAT1236020.3992385133089302No Hit
AATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAG1191870.3849779185268157No Hit
AGGTGCGGCTGGATCACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1116570.3606557715937867No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1099600.3551744059436738Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCACCGTGACCGCACCTTTCGGGGTGTGGCGCTGTAGGCACCATCAATA1091190.35245794836456656No Hit
CAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATC1027700.33195047016034335No Hit
CACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAATA1023000.3304323547475248No Hit
TGGTGGGGCTGCATGGGACCTTCGCTGTAGGCACCATCAATAGATCGGAA1019420.3292760030075481No Hit
CACCCGTTAACTCCCTTTGGGGGTGAAGCGGCTGTAGGCACCATCAATAG925630.29898152543983514No Hit
TGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGGA914830.2954930900231458No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATCTGTAGGCACCATCAAT846900.273551477258728No Hit
TAGTCGGTAACACCCGAAGCCGGTGGCCTCTGTAGGCACCATCAATAGAT846260.2733447551599612No Hit
AACGGGCTCTGGCGGGTGCGCGGGGTCCCCCTGTAGGCACCATCAATAGA793950.25644845361857016No Hit
TTGGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATC758190.24489785634997638No Hit
TTACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAA751710.24280479509996272No Hit
GGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCG717490.23175162288152648No Hit
TGTGTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGAT658660.21274933995894885No Hit
TTCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGA655440.2117092693995285No Hit
ACGGGCGTGGGGGTTCAAGTCCCCCCTCGGACACCACTGTAGGCACCATC639060.20641847568421626No Hit
ATAACCATCCTAAGGTAGCGAAATTCCTCTGTAGGCACCATCAATAGATC637120.20579184932232944No Hit
TGGTGGGGCTGCATGGGACCTTCGTTCTGTAGGCACCATCAATAGATCGG631290.20390874020387578No Hit
ATCAGCGGTACTAACCATCCAAAACCACCCTGTAGGCACCATCAATAGAT618040.1996289467528448No Hit
TAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATC592460.19136652286776007No Hit
AGGGGGACCCACATGGCGTGTAAGCCTCTGTAGGCACCATCAATAGATCG591400.1910241393916776No Hit
TAGTCGGTAACACCCGAAGCCGGTGGCCCTGTAGGCACCATCAATAGATC578040.18670881557992106No Hit
AACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC575920.1860240486277561No Hit
TAGGCGAATTCGGTGATCCTATGCTGCCGCTGTAGGCACCATCAATAGAT571630.18463836455946No Hit
ACTCCCCCAGGGCCGGAAGGCAGCAAGGGCTGTAGGCACCATCAATAGAT555750.1795090724838093No Hit
AGGGGGACCCACATGGCGTGTAAGCCCTGTAGGCACCATCAATAGATCGG528230.17062002223683775No Hit
TCAGCGGTACTAACCATCCAAAACCACCTGTAGGCACCATCAATAGATCG527270.17030993908868758No Hit
AGGTGCGGCTGGATCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAG523950.16923756820133493No Hit
TGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGATCGGA514860.166301468392288No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTCTGTAGGCACCATCAATAGAT479870.15499958364877295No Hit
TTGTCGAGCAGGGACGAAAGTCGGGACTAGCTGTAGGCACCATCAATAGA477250.15415331505694646No Hit
TCAGCGGTACTAACCATCCAAAACCACTGTAGGCACCATCAATAGATCGG465350.15030957603300163No Hit
TAACGGGCTCTGGCGGGTGCGCGGGGTCCCCCTGTAGGCACCATCAATAG447250.14446321667725365No Hit
GCCGCCTTAGCTCAGTCGGTAGAGCGATTCACCTGTAGGCACCATCAATA417920.1349895304947073No Hit
GGTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCGG410500.13259284616212993No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCACTGTAGGCACCATCAATA389130.12569026608299544No Hit
TTGGGGCTGCATGGGACCTTCGTTGCTGTAGGCACCATCAATAGATCGGA366780.11847114279012429No Hit
TATCAGCGGTACTAACCATCCAAAACCACCCTGTAGGCACCATCAATAGA363190.11731156101735438No Hit
ATGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG354650.11455311301193517No Hit
TTTGGTGGGGCTGCATGGGACCTTCGTTGGCTGTAGGCACCATCAATAGA351080.11339999130475173No Hit
GACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAAT350120.11308990815660155No Hit
ATAACCATCCTAAGGTAGCGAAATTCCCTGTAGGCACCATCAATAGATCG344690.11133600034987715No Hit
TCAGCGGTACTAACCATCCAAAACCACCGTCTGTAGGCACCATCAATAGA343560.11097100664424206No Hit
AGGTGCGGCTGGATCACCTCCTGTAGGCACCATCAATAGATCGGAAGAGC342970.11078043470944143No Hit
TGCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATCTGTAGGCACCATCAA340160.10987279549454353No Hit
TGGTGGGGCTGCATGGGACCTTCGTTGGTAGCTGTAGGCACCATCAATAG337410.10898453647640502No Hit
CAATCAGCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATA329930.10656847194706828No Hit
TTGGGGGTGAAGCGGAGAATTTAAGCCCCAGCTGTAGGCACCATCAATAG328350.1060581267657378No Hit
TTGCCGGTGCAGGCAAGAGACCACCGTAAGCGTCGTTGCCTGTAGGCACC328180.10600321620825288No Hit
TAGGCGTCTGGGGGGAACGCGGGGAAGTGCTGTAGGCACCATCAATAGAT322230.10408134669628046No Hit
TAGACTGTGAAGCTCACACGCCAGTGTGGGTGGAGTCGCTGTAGGCACCA321880.1039682955485174No Hit
GCGGTACTAACCATCCAAAACCACCGCTGTAGGCACCATCAATAGATCGG320940.10366467246595368No Hit
CTGTGTGCGGGGTTGTGGGACCTATCCTGTAGGCACCATCAATAGATCGG319820.10330290879311181No Hit
AAGGGGGACCCACATGGCGTGTAAGCCCTGTAGGCACCATCAATAGATCG315820.10201089567581945No Hit
TTACCCGTTAACTCCCTTTGGGGGTGAAGCGGACTGTAGGCACCATCAAT314860.10170081252766927No Hit
AACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGCTGTAGGCACCATCAAT310360.10024729777071534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)